Ecotoxicological Assessment of Solid Wastes: A Combined Liquid- And Solid-Phase Testing Approach Using a Battery of Bioassays and Biomarkers

Ferrari, B.; Radetski, Claudemir M.; Veber, Anne-Marie; Férard, Jean-François

doi:10.1897/1551-5028(1999)018<1195:eaoswa>2.3.co;2

Cited by 12 publications

(10 citation statements)

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“…From each homogenous mixture, leachate (100%) was prepared according to a standard procedure (ASTM, 1992 ; Ferrari et al, 1999 ). Briefly, 100 g of the sample (each homogenous mixture) was added to 100ml of distilled water (w/v) and shaken for 48hr at (30±1) °C.…”

Section: Methodsmentioning

confidence: 99%

Testicular membrane lipid damage by complex mixture of leachate from municipal battery recycling site as indication of idiopathic male infertility in rat

Akintunde¹,

Oboh²,

Akindahunsi³

2013

Interdisciplinary Toxicology

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Leachate from a municipal battery recycling site is a potent source of mixed-metal released into the environment. The present study investigated the degree at which mixed-metal exposure to the municipal auto-battery leachate (MABL) and to the Elewi Odo municipal auto-battery recycling site leachate (EOMABRL) affected the lipid membrane of the testes in in vitro experiment. The results showed elevated level of mixed-metals over the permissible levels in drinking water, as recommended by regulatory authorities. In the leachate samples, the levels of malondialdehyde (MDA), a biomarker of lipid damage, was significantly (p<0.05) increased in rat testes in a dose-dependent manner. MDA induced by the municipal auto-battery leachate (MABL) was significantly (p<0.05) higher than the leachate from Elewi Odo municipal auto-battery recycling site (EOMABRL). The testicular lipid membrane capacity was compromised following treatment with leachate from the municipal battery recycling site, implicating mixed-metal exposure as the causative agent of testicular damage and male infertility.

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Section: Methodsmentioning

confidence: 99%

Testicular membrane lipid damage by complex mixture of leachate from municipal battery recycling site as indication of idiopathic male infertility in rat

Akintunde¹,

Oboh²,

Akindahunsi³

2013

Interdisciplinary Toxicology

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“…Leachates of the solid waste (10%) were prepared according to the method described by Ferrari et al (29). In brief, 100g solid waste was added to 1000ml of distilled water, which was then continuously shaken for 24 hours at 30 ± 1°C.…”

Section: Preparation Of Leachatesmentioning

confidence: 99%

Municipal Sludge-induced Phytotoxicity

2005

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Municipal sludge (MS), containing various types of environmental pollutants, can exert phytotoxic effects in plants. Seed germination, chlorophyll content, plant weight, root and shoot growth, and metal levels, were measured as endpoints to assess the phytotoxic effects of leachates of MS from Lucknow city in three plants, Triticum aestivum, Brassica campestris and Phaseolus aureus. Among the five metals analysed, lead was found to be present at the highest concentration, and chromium at the lowest. Aqueous MS leachates had lower amounts of metals than pure MS. Seed germination was inhibited significantly in all three plants. The greatest inhibition of root and shoot growth was observed in B. campestris and T. aestivum. Total chlorophyll was depleted in all three plants, but the maximum depletion was observed in T. aestivum exposed to 10% leachate. Symptoms such as necrosis, leaf discoloration and root curling were also observed after exposure to 10% leachate, and plant weight was reduced significantly. It is evident that MS from Lucknow city exerted phytotoxic effects on all three test plant species.

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“…Solid waste leachates were prepared according to the French Standard method, as described by Ferrari et al (12). In brief, 100g solid waste was added to 1000ml distilled water to give a 10.0% leachate, which was then shaken continuously for 24 hours at 30 ± 1°C.…”

Section: Preparation Of Leachatesmentioning

confidence: 99%

Ecotoxicological Evaluation of Municipal Sludge

et al. 2005

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Municipal wastes originating from urban and industrial areas have become a major source of soil, ground and surface water pollution. These undesirable agents in our environment significantly interact with our flora and fauna. The aim of this study was to test samples of municipal sludge (MS) for their ecotoxicological potential by using sensitive bioassays involving a plant, Vicia faba, and the earthworm, Eisenia foetida. A 10% leachate of MS was prepared for the experiments, and V. faba seedlings were exposed to three leachate concentrations (2.5%, 5% and 10%) for 5 days. The findings revealed chromosome aberrations during the metaphase as well as the anaphase of cell division, and inhibition of the mitotic index, which reflects that MS originating from domestic and other human activities may be genotoxic to the living organisms of the ecosystem. Abnormalities in chlorophyll content, plant growth, root length, shoot length and root/shoot length ratio in V. faba clearly indicated the toxicity of the sludge. Behavioural and reproduction studies with E. foetida also provided evidence for the toxic nature of the MS.

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Ecotoxicological Assessment of Solid Wastes: A Combined Liquid- And Solid-Phase Testing Approach Using a Battery of Bioassays and Biomarkers

Cited by 12 publications

References 0 publications

Testicular membrane lipid damage by complex mixture of leachate from municipal battery recycling site as indication of idiopathic male infertility in rat

Testicular membrane lipid damage by complex mixture of leachate from municipal battery recycling site as indication of idiopathic male infertility in rat

Municipal Sludge-induced Phytotoxicity

Ecotoxicological Evaluation of Municipal Sludge

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