Ecotoxicological evaluation of zebrafish liver (Danio rerio) induced by dibutyl phthalate

Song, Peipei; Jiang, Nan; Zhang, Kaiqu; Li, Xianxu; Li, Na; Zhang, Youai; Wang, Qian; Wang, Jun

doi:10.1016/j.jhazmat.2021.128027

Cited by 64 publications

(17 citation statements)

References 56 publications

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“…Quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) results showed that the expression of FOSB in each of the five glioma cell lines was higher than that in the human astrocyte cell line at the mRNA level ( Figure 1E ). The discrepancy between protein and gene expression results was observed in the U118 glioma cell line and seems to be due to the time lag effect in protein modification, gene translation, and translation in this particular cell line (Song et al, 2022 ). Immunohistochemistry and immunofluorescence were used to detect the expression of FOSB in glioma tissues.…”

Section: Resultsmentioning

confidence: 99%

Expression and potential role of FOSB in glioma

Sun

Zheng

et al. 2022

Front. Mol. Neurosci.

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BackgroundFOSB is reported to be an oncogene in a variety of tumors. However, the expression and role of FOSB in glioma remain obscure. In this study, we aimed to explore the expression of FOSB in glioma and its biological role in glioblastoma multiforme (GBM).MethodsWestern blot, immunohistochemical staining, and quantitative real-time polymerase chain reaction (RT-qPCR) were used to detect the expression of FOSB in clinical samples. FOSB was knocked down in cells to determine the effects of FOSB on the phenotypic changes of tumors by plate cloning, CCK-8 assay, and Transwell assay. Finally, subcutaneous tumorigenesis in nude mice was used to observe the tumorigenesis of glioma cell lines after the knockdown of the FOSB gene.ResultsFOSB expression was higher in glioma compared with normal brain tissue. After the downregulation of FOSB, the expression of cleaved caspase-3 increased. Plate cloning and CCK-8 experiments showed that the proliferation of glioma cell lines decreased. The Transwell assay demonstrated that the glioblastoma cell lines had lower migration ability after the knockdown of FOSB. Finally, the tumor volume of U87 glioma cells in group sh-FOSB was smaller than that in the control group. The TUNEL staining in vitro showed that the apoptosis of sh-FOSB glioma cells increased.ConclusionFOSB was highly expressed in glioma tissues. The viability of glioma cells decreased, and the ability of glioma cells to proliferate and migrate was reduced when FOSB was downregulated. Hence, FOSB may promote the development and migration of gliomas.

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Section: Resultsmentioning

confidence: 99%

Expression and potential role of FOSB in glioma

Sun

Zheng

et al. 2022

Front. Mol. Neurosci.

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“…Although the underlying mechanism is not well understood, data suggested that ROS and/or oxidative stress was involved in their diverse effect on mRNA and protein expression levels. For an example, Song et al (2022) reported that, due to the time lag effect in protein modification, gene transcription and translation, the expressions of SOD and GPX subtype genes did not change in accordance with those at protein levels. It was presumed that the oxidative stress or altered ROS level perturbed mRNA translation efficiency, and also affected protein degradation rates and the folding and modification efficiency of proteins, e.g., in mitochondria and nucleus (Tan et al, 2017;Latonen et al, 2018;Sun et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Integrative Metabolomics, Proteomics and Transcriptomics Analysis Reveals Liver Toxicity of Mesoporous Silica Nanoparticles

Sun

et al. 2022

Front. Pharmacol.

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As pharmaceutical excipients, mesoporous silica nanoparticles (MSNs) have attracted considerable concern based on potential risks to the public. The impact of MSNs on biochemical metabolism is poorly understood, and few studies have compared the effects of MSNs administered via different routes. To evaluate the hepatotoxicity of MSNs, metabolomics, proteomics and transcriptomic analyses were performed in mice after intravenous (20 mg/kg/d) or oral ad-ministration (200 mg/kg/d) of MSNs for 10 days. Intravenous injection induced significant hepatic injury based on pathological inspection and increased the levels of AST/ALT and the inflammatory factors IL-6, IL-1β and TNF-a. Omics data suggested intravenous administration of MSNs perturbed the following metabolites: succinate, hypoxanthine, GSSG, NADP+, NADPH and 6-phosphogluconic acid. In addition, increases in GPX, SOD3, G6PD, HK, and PFK at proteomic and transcriptomic levels suggested elevation of glycolysis and pentose phosphate pathway, synthesis of glutathione and nucleotides, and antioxidative pathway activity, whereas oxidative phosphorylation, TCA and mitochondrial energy metabolism were reduced. On the other hand, oral administration of MSNs disturbed inflammatory factors and metabolites of ribose-5-phosphate, 6-phosphogluconate, GSSG, and NADP+ associated with the pentose phosphate pathway, glutathione synthesis and oxidative stress albeit to a lesser extent than intravenous injection despite the administration of a ten-fold greater dose. Overall, systematic biological data suggested that intravenous injection of nanoparticles of pharmaceutical excipients substantially affected hepatic metabolism function and induced oxidative stress and inflammation, whereas oral administration exhibited milder effects compared with intravenous injection.

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“…It has been shown that the most critical aspects of the adaptation strategy of fish exposed to prolonged hypoxic levels is the adjustment of energy metabolism (Krumschnabel et al, 2000;Xiao, 2015). A recent study discovered that prolonged hypoxia significantly regulated the fatty acid metabolic pathway in zebrafish (Danio rerio) liver (Song et al, 2022). In addition, hypoxia inhibits the production of acetyl coenzyme A, which is required for fatty acid oxidation (Fuhrmann et al, 2019).…”

Section: Changes In Steroid Synthesis Ppar Signaling and Fatty Acid S...mentioning

confidence: 99%

Histological, microecological and transcriptomic physiological responses underlying hypoxia and reoxygenation adaptation in yellowtail kingfish (Seriola lalandi)

Zhou

Jiang²,

Xu³

et al. 2023

Front. Mar. Sci.

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Yellowtail kingfish has emerged as one of the most promising marine fishes for aquaculture in China because it is tasty, fast growing, and has high economic value. To investigate the tolerance and adaptability to hypoxia of farmed yellowtail kingfish, juveniles were exposed to hypoxia (3.0 ± 0.5 mg/L) for 5 days and then returned to normoxia (7.5 ± 0.5 mg/L) for another 5 days. Using tissue sections and high-throughput sequencing technology, we investigated the histological, microecological, transcriptomic, and physiological adaptation mechanisms of yellowtail kingfish. The results showed that hypoxia increased the gill lamellae length and spacing, which were reversible post-reoxygenation. At the genus level, the relative abundances of Prevotella, Bacteroides, Roseburia, and Blautia in the gastrointestinal tract increased under hypoxia and were maintained post-reoxygenation. The liver transcriptome revealed that, compared with normoxia group, the different expression genes (DEGs) were mainly enriched in Steroid biosynthesis and PPAR signaling pathways in hypoxia group. Compared with normoxia group, the DEGs were mainly enriched in Ribosome biogenesis in eukaryotes, Steroid biosynthesis, Fatty acid biosynthesis, and PPAR signaling pathways in reoxygenation group. Furthermore, compared with hypoxia group, the DEGs were mainly enriched in Ribosome biogenesis in eukaryotes and Ribosome pathways in reoxygenation group. In contrast to normoxia, of the key genes of the PPAR signaling pathway, FABP4 was significantly downregulated, and SCD-1 and FATP were significantly upregulated. These findings indicated reduced lipid deposition and increased lipid decomposition in liver under hypoxia. The genes including PPARα, SCD-1, ANGPTL4, and FASN were significantly upregulated in lipid metabolism-related pathways, which indicated that lipid metabolism activity was more vigorous during reoxygenation. In contrast to the hypoxia group, almost all of the genes involved in Ribosome biogenesis in eukaryotes and Ribosome pathways for protein processing were significantly upregulated during reoxygenation; this is probably related to the clearance of misfolded proteins and the folding of the new proteins repairing there is damage to the body. The present results shed light on the possible synergetic function of lipid metabolism, protein repairment and synthesis, and gastrointestinal microbiota in resistance and homeostasis maintenance of yellowtail kingfish coping with hypoxic stress in aquaculture.

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Ecotoxicological evaluation of zebrafish liver (Danio rerio) induced by dibutyl phthalate

Cited by 64 publications

References 56 publications

Expression and potential role of FOSB in glioma

Expression and potential role of FOSB in glioma

Integrative Metabolomics, Proteomics and Transcriptomics Analysis Reveals Liver Toxicity of Mesoporous Silica Nanoparticles

Histological, microecological and transcriptomic physiological responses underlying hypoxia and reoxygenation adaptation in yellowtail kingfish (Seriola lalandi)

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