Ectoine accumulation in Brevibacterium epidermis

Onraedt, Annelies; Muynck, Cassandra De; Walcarius, Bart; Soetaert, Wim; Vandamme, Erick

doi:10.1023/b:bile.0000044448.86907.e4

Cited by 26 publications

(15 citation statements)

References 13 publications

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“…For instance, the genus Brevibacterium accumulates 150-160 mg g biomass -1 depending on the specie, while ectoine yields of 155-200 mg g biomass -1 have been recorded in Halomonas sp.. Higher yields can be achieved by genetically modified microorganisms (Escherichia coli DH5α can synthesize up to 400-540 mg g biomass -1 ) [8,11,12,[21][22][23]. Nevertheless, M. alcaliphilum 20Z exhibits a superior environmental performance based on its ability to produce ectoine from dilute CH 4 emissions, with a concomitant mitigation of climate change.…”

Section: <Fig 4>mentioning

confidence: 99%

Ectoine bio-milking in methanotrophs: A step further towards methane-based bio-refineries into high added-value products

Cantera

Lebrero

Rodríguez

et al. 2017

Chemical Engineering Journal

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Abstract:This communication showed for the first time that the methanotrophic strain Methylomicrobium alcaliphilum 20Z can efficiently synthesize and excrete (through a tailored bio-milking process) ectoine under continuous mode using methane as the sole energy and carbon source. First, three consecutive 50 h fed batch fermentations consisting of alternating high salinity (6% NaCl for 24h) and low salinity (0 % NaCl for 24h) cultivation stages were carried out in triplicate to determine the influence of sudden modifications in media salinity on ectoine synthesis and excretion. The results demonstrated that M. alcaliphilum 20Z exhibited a rapid response to osmotic shocks, which resulted in the release of the accumulated ectoine under hyposmotic shocks and the immediate uptake of the previously excreted ectoine during hyperosmotic shocks. A second experiment was carried out under continuous cultivation mode in two sequential stirred tank reactors operated at NaCl concentrations of 0 and 6 %. Cells exhibited a constant intracellular ectoine concentration of 70.4 ± 14.3 mg g biomass -1 along the entire operation period when cultivated at a NaCl concentration of 6%. The centrifugation of the cultivation broth followed by a hyposmotic shock resulted in the excretion of ~ 70 % of the total intracellular ectoine. In brief, this research shows the feasibility of the continuous bioconversion of diluted CH 4 emissions into high added-value products such as ectoine, which can turn greenhouse gas abatement into a sustainable and profitable process.

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Section: <Fig 4>mentioning

confidence: 99%

Ectoine bio-milking in methanotrophs: A step further towards methane-based bio-refineries into high added-value products

Cantera

Lebrero

Rodríguez

et al. 2017

Chemical Engineering Journal

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“…In Chromohalobacter israelensis (formerly Bacterium Ba1), ectoine only accumulated when the cells were grown in greater than 0.6 M NaCl, and only in exponentially growing cells [26]. Some microorganisms, e.g., Brevibacterium epidermidis , can also metabolize ectoine [27], perhaps a useful trait if external salt concentrations decrease. In that halotolerant organism, ectoine accumulation occurs only with salt stress, not sugar stress.…”

Section: Identification Of Osmolytesmentioning

confidence: 99%

Organic compatible solutes of halotolerant and halophilic microorganisms

2005

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Microorganisms that adapt to moderate and high salt environments use a variety of solutes, organic and inorganic, to counter external osmotic pressure. The organic solutes can be zwitterionic, noncharged, or anionic (along with an inorganic cation such as K(+)). The range of solutes, their diverse biosynthetic pathways, and physical properties of the solutes that effect molecular stability are reviewed.

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“…In Bacillus pasteurii (Kuhlmann and Bremer, 2002), Marinococcus halophilus (Bestvater and Galinski, 2002), Halobacillus halophilus (Saum and Muller, 2008b) and S. salexigens (Bursy et al, 2007), transcription of the ectABC-ask or ectABC genes proceeded as polycistronic mRNAs and subjected to osmotic conditions. The osmoregulated expression of ectABC was also revealed in gramnegative Brevibacterium epidermis (Onraedt et al, 2004), Mm alcaliphilum (Reshetnikov et al, 2006;Mustakhimov et al, 2010) and Chromohalobacter salexigens (Calderon et al, 2004).…”

Section: Ectoine Synthase (Ectc Ec 421108)mentioning

confidence: 84%

Genetic and Biochemical Aspects of Ectoine Biosynthesis in Moderately Halophilic and Halotolerant Methylotrophic Bacteria

Khmelenina

Mustakhimov

Reshetnikov

et al. 2010

American Journal of Agricultural and Biological Sciences

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Problem statement:The cyclic imino acid ectoine is a widely distributed compatible solute synthesizing by halophilic and halotolerant bacteria to prevent osmotic stress at high external salinity. This water-keeping compound is used in a variety of commercial cosmetics and therapeutic products. Approach: Development of integrated, predictive functional model of the metabolic and regulatory netwoks of ectoine-producing microbes is an active area of research. In this article we present a brief overview of the current knowledge on genetic and biochemical aspects of ectoine biosynthesis in aerobic halophilic and halotolerant bacteria utilizing C 1 compounds (methylotrophs). Although enzymology and genetics of the ectoine biosynthesis in methylotrophs are similar to other halophilic bacteria, the regulatory patterns are different. In all methylotrophic bacteria studied, the genes coding for specific enzymes of ectoine biosynthesis: Diaminobutyric Acid (DABA) aminotransferase (EctB), DABA acetyltransferase (EctA) and ectoine synthase (EctC) are organized into ectABC or ectABC-ask, whith is linked to gene encoding Aspartokinase isozyme (Ask). Results: Remarkably, the methylotrophic bacteria possessing a four-gene cluster showed higher halotolerance and accumulated more ectoine than bacteria with a cluster composed of three genes. The DABA acetyltransferases from three methylotrophic species have been comparatively characterized. The properties of the enzymes correlate with eco-physiological and metabolic particularities of the host. Some elements of the regulatory system governing the ectoine pathway operation have been revealed in both methane and methanol utilizing bacteria. In Methylomicrobium alcaliphilum transcription of the ectABC-ask operon is initiated from two σ 70 -like promoters and controlled by the EctR, a MarR-type negative regulator. EctR orthologs were identified in genomes of several heterotrophic halophilic bacteria. Here we present genomic data indicating that similar regulatory system may occur in diverse halophilic and halotolerant bacteria. Conclusion: Currently available data suggest that in methylotrophic bacteria the ectoine biosynthesis pathways are evolutionary well conserved, particularly with respect to the genes and enzymes involved. However, some differences in the ect-gene cluster organization and regulation could be observed.

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Ectoine accumulation in Brevibacterium epidermis

Cited by 26 publications

References 13 publications

Ectoine bio-milking in methanotrophs: A step further towards methane-based bio-refineries into high added-value products

Ectoine bio-milking in methanotrophs: A step further towards methane-based bio-refineries into high added-value products

Organic compatible solutes of halotolerant and halophilic microorganisms

Genetic and Biochemical Aspects of Ectoine Biosynthesis in Moderately Halophilic and Halotolerant Methylotrophic Bacteria

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