Ectomycorrhization of Tricholoma matsutake with Abies veitchii and Tsuga diversifolia in the subalpine forests of Japan

Abstract

Matsutake mushroom Mycorrhizal isolationNuclear ribosomal RNA gene (rDNA) IGS1 region a b s t r a c t Tricholoma matsutake is one of the most highly valued ectomycorrhizal mushrooms, primarily associated with conifers. Here, we examined the association of T. matsutake with hemlock and fir species native to the subalpine forests of Japan. Basidiomata of T. matsutake were harvested from the forests of Honshu Island, Japan, along with two soil samples directly beneath the basidiomata; ectomycorrhizal root tips were also collected, and used for fungal isolation and molecular analyses. Mycorrhizal fungi were isolated from the roots of Abies veitchii (Veitch's fir) and Tsuga diversifolia (northern Japanese hemlock). These fungi were identified as T. matsutake based upon their production of whitish mycelial colonies on nutrient agar media, and confirmed using molecular analyses; the ectomycorrhizal association between T. matsutake and A. veitchii was also confirmed by mycorrhizal synthesis in vitro. This work represents the first description of T. matsutake ectomycorrhizations withAbies and Tsuga species, as well as a detailed morphological description of these associations. /locate /m yc m y c o s c i e n c e 5 6 ( 2 0 1 5 ) 4 0 2 e4 1 2 http://dx.

Biogeography of the Japanese Gourmet Fungus, Tricholoma matsutake: A Review of the Distribution and Functional Ecology of Matsutake

“…In general, host-plant genotype is believed to determine root colonization, ecological fitness, and metabolic activity of EcM fungi as well as the outcome of competitive interactions between two or more EcM fungi colonizing the same host (Bryla and Koide 1990;Tagu et al 2005;Courty et al 2011). In line with natural observations, in vitro trials have shown that matsutake can form root symbioses with conifers such as Pinus, Picea, Abies and Tsuga (Yamada et al 1999(Yamada et al , 2014Gill et al 2000;Vaario et al 2010;Endo et al 2015), as well as form partial associations with other plants (e.g., Larix kaempferi, Cedrela odorata, Prunus spp., Betula platyphylla var. japonica and Populus tremula  tremuloides), but these have not been confirmed in natural settings (Murata et al 2013a(Murata et al , 2014a(Murata et al , b, 2015bYamada et al 2014).…”

“…In the deep mountainous terrain of Honshu, Tsuga siebordii and T. diversfolia are the main ectomycorrhizal hosts of T. matsutake in temperate and subalpine climates, respectively (Hamada 1964;Ogawa 1976bOgawa , 1977aEndo et al 2015). At the edge of the range of P. densiflora in Hokkaido, P. pumila, Picea glehnii, and Abies sachalinensis serve as hosts of T. matsutake in alpine, alpine-subalpine, and subalpine climates, respectively (Hamada 1964;Ogawa 1976a, b;Murata and Minamide 1989;Endo et al 2015). Japanese subalpine forests are quite diverse in terms of conifers, especially on Honshu where pines, firs, spruces, hemlocks, a larch, and a false hemlock can be found.…”

“…Japanese subalpine forests are quite diverse in terms of conifers, especially on Honshu where pines, firs, spruces, hemlocks, a larch, and a false hemlock can be found. Unfortunately, little is known of their respective roles as host trees for T. matsutake, although Abies veitchii was recently confirmed as an alternative host (Endo et al 2015). Given that the association between firs and T. matsutake has been confirmed in Japan, a comprehensive survey of host-tree use for populations in China and Fennoscandia should be performed.…”

“…In western Honshu, this temperature was shown to be a good indicator of fruiting (Ogawa 1978). However, in Nagano and Iwate Prefectures, some populations were believed to fruit at lower temperatures (Narimatsu et al 2015;Endo et al 2015) because the cool temperate and subalpine forests experience lower soil temperatures. Similarly, soil temperature at first fruiting is much lower based on a 6-year survey in southern Finland (Vaario et al 2015c).…”

Metabolic Profiling of Water-Soluble Compounds from the Extracts of Dark Septate Endophytic Fungi (DSE) Isolated from Scots Pine (Pinus sylvestris L.) Seedlings Using UPLC–Orbitrap–MS

“…However, the strain A had 100% identity together with Acephala applanata strain (AY078147.1), whereas strain R was more similar with Phialocephala fortinii strains (see alignments in Supplementary Figure S2). Humicolopsis cephalosporioides and Coniochaeta mutabilis can be considered as DSE-like fungi [19]. Previously, Coniochaeta were considered as Lecytophora sp.…”