Ectopic bone formation as a complication of surgical rehabilitation in patients with Moebius’ syndrome

Franz, Marcus; Berndt, Alexander; Wehrhan, Falk; Schleier, Peter; Clement, Joachim H.; Hyckel, Peter

doi:10.1016/j.jcms.2007.05.002

Cited by 4 publications

(3 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even today tissue mineralization during bone formation is not fully understood (36) but evidence is increasing that mineralization per se is not a challenge [as illustrated by ectopic bone formation (37–39), fibrodysplasia ossificans progressiva, or uncontrolled bone formation after limb amputation (40)]. For healthy functional bone to form and to harbor the best quality to withstand strains and stresses of daily life, a controlled, structure-optimizing, and directed bone formation is key to success.…”

Section: Discussionmentioning

confidence: 99%

T Lymphocytes Influence the Mineralization Process of Bone

et al. 2017

View full text Add to dashboard Cite

Bone is a unique organ able to regenerate itself after injuries. This regeneration requires the local interplay between different biological systems such as inflammation and matrix formation. Structural reconstitution is initiated by an inflammatory response orchestrated by the host immune system. However, the individual role of T cells and B cells in regeneration and their relationship to bone tissue reconstitution remain unknown. Comparing bone and fracture healing in animals with and without mature T and B cells revealed the essential role of these immune cells in determining the tissue mineralization and thus the bone quality. Bone without mature T and B cells is stiffer when compared to wild-type bone thus lacking the elasticity that helps to absorb forces, thus preventing fractures. In-depth analysis showed dysregulations in collagen deposition and osteoblast distribution upon lack of mature T and B cells. These changes in matrix deposition have been correlated with T cells rather than B cells within this study. This work presents, for the first time, a direct link between immune cells and matrix formation during bone healing after fracture. It illustrates specifically the role of T cells in the collagen organization process and the lack thereof in the absence of T cells.

show abstract

Section: Discussionmentioning

confidence: 99%

T Lymphocytes Influence the Mineralization Process of Bone

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Bone morphogenetic proteins (BMPs) are a group of classic bone growth factors that can exert osteogenic effects on a broad spectrum of cell types. At the same time, BMPs have been found to be involved in the development of numerous tissues and organs (2,3), and this nonspecificity of BMPs usually results in unexpected side effects (4,5). Thus, it is imperative to identify growth factors with fewer side effects and favorable specificity as an alternative to BMPs.…”

Section: Introductionmentioning

confidence: 99%

Osteogenic differentiation of human periodontal ligament stem cells expressing lentiviral NEL-like protein 1

Chen

Liu

Shi

et al. 2012

International Journal of Molecular Medicine

View full text Add to dashboard Cite

NEL-like protein 1 (NELL1) is a newly identified secreted protein involved in craniosynostosis and has been found to promote osteogenic differentiation of mesenchymal stem cells. The objective of this study was to investigate the effect of NELL1 on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) and the potential underlying mechanism. hPDLSCs underwent lentivirus-mediated NELL1 transfection (Lenti-NELL1) and markers of osteogenesis were assessed [alkaline phosphate (ALP), osteocalcin (OCN) and calcium deposition] to evaluate the effect of NELL1 on the differentiation of these cells. Quantitative polymerase chain reaction (qPCR) was employed to measure the mRNA expression of Msx2 and Runx2, and Lenti-enhanced green fluorescent protein (EGFP) served as a control. Western blot analysis and qPCR analyses confirmed that Lenti-NELL1-transfected hPDLSCs could express NELL1. Compared with the Lenti-EGFP group, ALP, OCN, calcium deposition and Msx2 mRNA expression were markedly increased (P<0.01), but there was no significant difference in Runx2 mRNA expression between the two groups (P>0.01). hPDLSCs can be transfected by Lenti-NELL1 and can stably express NELL1. NELL1 is able to promote the osteogenic differentiation of hPDLSCs, which may be related to the downregulation of Msx2 expression. Lenti-NELL1 transfection can be used during in vitro gene therapy for periodontal regeneration.

show abstract

“…It should be considered when crossover facial nerve reinnervation or grafting is not possible. However, ectopic bone formation in Möbius patients, in particular after muscular neurotization, has been reported (Franz et al, 2007).…”

Section: Discussionmentioning

confidence: 99%