Ectopic coexpression of keratin 8 and 18 promotes invasion of transformed keratinocytes and is induced in patients with cutaneous squamous cell carcinoma

Yamashiro, Yoshito; Takei, Kimiko; Umikawa, Masato; Asato, Tsuyoshi; Oshiro, Minoru; Uechi, Yukiko; Ishikawa, Takahiro; Taira, Kaoru; Uezato, Hiroshi; Kariya, Ken-ichi

doi:10.1016/j.bbrc.2010.07.077

Cited by 24 publications

(23 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The resultant stable transfectants that expressed vimentin and the two CKs, showed a two to three-fold increase in their invasion of basement membrane matrix and migration through gelatin in vitro compared to nontransfected cells (Chu et al, 1996). Similarly, Yamashiro et al also reported that coexpression of exogenous CK8 and CK18 conferred invasiveness to the parental non-metastatic transformed mouse keratinocyte line (Yamashiro et al, 2010).…”

Section: Wwwintechopencommentioning

confidence: 82%

“…Several research groups have reported that coexpression of CK8, CK18 and type III IF vimentin confer highly invasive phenotypes on various tumors (Chu et al, 1993;Chu et al, 1996;Chu et al, 1997;Yamashiro et al, 2010). However, observations that are apparently contradictory to those mentioned above have also been reported; decreased expression of type I CKs significantly correlated with tumor progression or increased invasion ability in several other tumor types (Buhler & Schaller, 2005;Crowe et al, 1999;Woelfle et al, 2004).…”

Section: Cytokeratins and Lung Cancermentioning

confidence: 99%

“…In addition, it has been reported that CKs play a role in the regulation of cancer cell invasion, metastasis and tumorigenesis (Buhler & Schaller, 2005;Chu et al, 1993;Chu et al, 1996;Chu et al, 1997;Crowe et al, 1999;Hendrix et al, 1992;Kanaji et al, 2011;Schaller et al, 1996;Woelfle et al, 2004;Yamashiro et al, 2010). Table 1 In 1992, expression levels of CK8 and CK18 were determined in nine human melanoma cell lines expressing vimentin.…”

Section: Relationship Between Cytokeratin Expression Levels and Cancementioning

confidence: 99%

“…CK8 and 18 are not expressed in normal keratinocytes. However, both CKs are co-expressed in a metastatic derivative of a transformed mouse keratinocyte cell line in which they formed CK8/18 filaments (Yamashiro et al, 2010). In addition, analysis of specimens from 21 pre-invasive and 24 invasive cutaneous squamous cell carcinomas (cSCCs) derived from patients indicated that ectopic CK8/18 coexpression was almost exclusively detected in invasive cSCCs (Yamashiro et al, 2010).…”

Section: Relationship Between Cytokeratin Expression Levels and Cancementioning

confidence: 99%

See 3 more Smart Citations

Cytokeratin 8: The Dominant Type II Intermediate Filament Protein in Lung Cancer

Kanaji¹,

Kubo²,

Bandoh³

et al. 2012

Cytokeratins - Tools in Oncology

View full text Add to dashboard Cite

Section: Wwwintechopencommentioning

confidence: 82%

Section: Cytokeratins and Lung Cancermentioning

confidence: 99%

Section: Relationship Between Cytokeratin Expression Levels and Cancementioning

confidence: 99%

Section: Relationship Between Cytokeratin Expression Levels and Cancementioning

confidence: 99%

See 2 more Smart Citations

Cytokeratin 8: The Dominant Type II Intermediate Filament Protein in Lung Cancer

Kanaji¹,

Kubo²,

Bandoh³

et al. 2012

Cytokeratins - Tools in Oncology

View full text Add to dashboard Cite

“…In addition, CKs have been reported to play an important role in the regulation of cell invasion and tumorigenesis (5)(6)(7)(8)(9)(10)(11). In CK19-deficient oral squamous cell carcinoma lines, stable expression of CK19 significantly decreased invasive ability (5).…”

Section: Introductionmentioning

confidence: 99%

Cytokeratins negatively regulate the invasive potential of lung cancer cell lines

Kanaji¹

2011

Oncol Rep

View full text Add to dashboard Cite

Abstract. Lung cancer cells express several cytokeratins (CKs) that are subdivided into type I (CK9-23) and type II (CK1-8) subclasses. The functions of CKs in lung cancer cells have not been fully elucidated. The purpose of this study was to investigate the role of CKs in the invasion of lung cancer cells. We investigated the expression levels of CK7, 8, 18 and 19 in 12 non-small cell lung cancer (NSCLC) and seven SCLC cell lines by quantitative immunoblotting. The expression levels of these four CKs were significantly higher in the NSCLC cells. The NSCLC cell line HI1017 expressed CK8 and 18; A549 cells expressed CK7, 8, 18 and 19, respectively. Invasive sublines of HI1017 and A549 were established by repeated selection of invasive cells using a membrane invasion chamber system. The invasive cell lines showed lower expression levels of CKs compared with the parental cells. Exogenous CK19 also resulted in a decrease in invasiveness of the HI1017 cells. Suppression of either CK8 or CK18 by short interfering RNAs led to a decrease in the total CKs and increased invasiveness of both the HI1017 and A549 cells. A549 cells expressed very low levels of CK19. Suppression of CK19 affected neither invasive ability nor total CK amount in the A549 cells. Our observations indicate that CK expression levels were inversely associated with invasiveness of the NSCLC cell lines, and suggest that expression levels of dominant CKs may affect invasive ability.

show abstract

Epidermal growth factor-induced modulation of cytokeratin expression levels influences the morphological phenotype of head and neck squamous cell carcinoma cells

et al. 2012

View full text Add to dashboard Cite

The migratory ability of tumor cells requires cytoskeletal rearrangement processes. Epidermal growth factor receptor (EGFR)-signaling tightly correlates with tumor progression in head and neck squamous cell carcinomas (HNSCCs), and has previously been implicated in the regulation of cytokeratin (CK) expression. In this study, HNSCC cell lines were treated with EGF, and CK expression levels were monitored by Western blot analysis. Changes in cellular morphology were documented by fluorescence- and atomic force microscopy. Some of the cell lines demonstrated an EGF-dependent modulation of CK expression levels. Interestingly, regression of some CK subtypes or initial up-regulation followed by downregulation at higher EGF-levels could also be observed in the tested cell lines. Overall, the influence of EGF on CK expression levels appeared variable and cell-type-dependent. Real-time cellular analysis of EGF-treated and -untreated HNSCC cell lines demonstrated a rise over time in cellular impedance. In three of the EGF-treated HNSCC cell lines, this rise was markedly higher than in untreated controls, whereas in one of the cell lines the gain of cellular impedance was paradoxically reduced after EGF treatment, which was found to correlate with changes in cellular morphology rather than with relevant changes in cellular viability or proliferation. After treating HNSCC cells with EGF, CK filaments frequently appeared diffusely distributed throughout the cytoplasm, and in some cases were found in a perinuclear localization, the latter being reminiscent to observations by other groups. In summary, the data points to a possible role of EGFR in modulating HNSCC cell morphology.

show abstract

Ectopic coexpression of keratin 8 and 18 promotes invasion of transformed keratinocytes and is induced in patients with cutaneous squamous cell carcinoma

Cited by 24 publications

References 19 publications

Cytokeratin 8: The Dominant Type II Intermediate Filament Protein in Lung Cancer

Cytokeratin 8: The Dominant Type II Intermediate Filament Protein in Lung Cancer

Cytokeratins negatively regulate the invasive potential of lung cancer cell lines

Epidermal growth factor-induced modulation of cytokeratin expression levels influences the morphological phenotype of head and neck squamous cell carcinoma cells

Contact Info

Product

Resources

About