Ectopic expression of a maize calreticulin mitigates calcium deficiency-like disorders in sCAX1-expressing tobacco and tomato

Wu, Qingyu; Shigaki, Toshiro; Han, Jeung-Sul; Kim, Chang Kil; Hirschi, Kendal D.; Park, Sunghun

doi:10.1007/s11103-012-9970-6

Cited by 12 publications

(11 citation statements)

References 49 publications

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“…The effects of the overexpression of sCAX1 in the Atlantic lines were the same observed in the Russet Norkotah lines overexpressing sCAX1 developed by Park et al (2005a). Our results are in agreement with reports in other crops showing that overexpressing sCAX1 causes Ca 2+ deficiency symptoms (de Freitas et al, 2011;Wu et al, 2012).…”

Section: Cell Wall Content In Tubers Decreases As a Consequence Of Ovsupporting

confidence: 91%

Section: Calcium Supplementation Improves Deficiency Symptoms In Apicmentioning

confidence: 99%

“…A recent study demonstrated that some Ca 2+ deficiency symptoms in maize (Zea mays L.) can be relieved by the expression of a gene encoding for the Ca 2+ binding protein calreticulin (Wu et al, 2012), a gene that might be involved in symplastic Ca 2+ mobilization. This experiment was done in tobacco and tomato where the co-expression of calreticulin, a chaperone found in the endoplasmic reticulum, in the sCAX1-expressing lines relieved the Ca 2+ deficiency symptoms caused by sCAX1 (Wu et al, 2012). Therefore, we may hypothesize that if Atlantic and Russet Norkotah sCAX1 lines were co-transformed with calreticulin, Ca 2+ deficiency symptoms might be reduced and total Ca 2+ increased; however, the success of this experiment would depend on a tissue and time-specific promoters used for the expression of these genes.…”

Section: Calcium Supplementation Improves Deficiency Symptoms In Apicmentioning

confidence: 99%

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CAX1 Vacuolar Antiporter Overexpression in Potato Results in Calcium Deficiency in Leaves and Tubers by Sequestering Calcium as Calcium Oxalate

et al. 2019

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Recent studies show that overexpression of a short version of the cation exchanger 1 gene (sCAX1) can cause Ca2+ deficiency symptoms in tomato (Solanum lycopersicum L.). However, the Ca2+ deficiency in relation to the overexpression of this gene has not been investigated in potato (Solanum tuberosum L.). The objective of our study was to investigate the production of known Ca2+ deficiency symptoms in potato in relation to the overexpression of sCAX1. Plantlets of S. tuberosum cultivar ‘Atlantic’ overexpressing the sCAX1 gene were produced using Agrobacterium tumefaciens. Transgenic plants grown with normal amounts of Ca2+ under in vitro or greenhouse conditions showed known Ca2+ deficiency symptoms in potato plants such as shoot tips damage and leaf margin necrosis, as well as tuber internal defects (hollow heart). Growing the transgenic plants with higher amounts of Ca2+ in the media or soil nutrient solution mitigated these symptoms. These results support the notion that both shoot tip necrosis and hollow heart are associated with Ca2+ deficiency. There was abundance of calcium oxalate (CaC2O4) crystals present only in the transgenic plants suggesting that these plants sequester Ca2+ in the form of CaC2O4 in the vacuoles of transgenic plants, reducing Ca2+ in the other pools. Since both shoot tip necrosis and hollow heart are known to be associated with poor cell wall health, our results suggest that CAX1 is a regulator of Ca2+ in the cell wall. In support of this concept, we found reduced cell wall biomass in the transgenic plants compared with the wild type.

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Section: Cell Wall Content In Tubers Decreases As a Consequence Of Ovsupporting

confidence: 91%

Section: Calcium Supplementation Improves Deficiency Symptoms In Apicmentioning

confidence: 99%

Section: Calcium Supplementation Improves Deficiency Symptoms In Apicmentioning

confidence: 99%

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CAX1 Vacuolar Antiporter Overexpression in Potato Results in Calcium Deficiency in Leaves and Tubers by Sequestering Calcium as Calcium Oxalate

et al. 2019

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“…The expression of sCAX1 was shown to reduce apoplastic Ca 2+ levels, which increased membrane leakiness [234]. Coexpression of CRT resulted in a significant decrease in Ca 2+ deficiency symptoms in both tomato and tobacco without the addition of supplemental Ca 2+ [235]. This is very interesting and, if it can be repeated in other species, may suggest several things about the ER and vacuole with respect to signaling.…”

Section: Coexpression Of Scax1 and Crt1mentioning

confidence: 75%

Modulating Plant Calcium for Better Nutrition and Stress Tolerance

Robertson

2013

ISRN Botany

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External Ca2+ supplementation helps plants to recover from stress. This paper considers genetic methods for increasing Ca2+ to augment stress tolerance in plants and to increase their nutritional value. The transport of Ca2+ must be carefully controlled to minimize fluctuations in the cytosol while providing both structural support to new cell walls and membranes, and intracellular stores of Ca2+ for signaling. It is not clear how this is accomplished in meristems, which are remote from active transpiration—the driving force for Ca2+ movement into shoots. Meristems have high levels of calreticulin (CRT), which bind a 50-fold excess of Ca2+ and may facilitate Ca2+ transport between cells across plasmodesmatal ER. Transgenes based on the high-capacity Ca2+-binding C-domain of CRT1 have increased the total plant Ca2+ by 15%–25% and also increased the abiotic stress tolerance. These results are compared to the overexpression of sCAX1, which not only increased total Ca2+ up to 3-fold but also caused Ca2+ deficiency symptoms. Coexpression of sCAX1 and CRT1 resolved the symptoms and led to high levels of Ca2+ without Ca2+ supplementation. These results imply an important role for ER Ca2+ in stress tolerance and signaling and demonstrate the feasibility of using Ca2+-modulating proteins to enhance both agronomic and nutritional properties.

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“…Wu et al [53] investigated the occurrence of Ca 2+ deficiency disorders and Ca 2+ accumulation in both sCAX1-expressing plants and sCAX1 and CRT-co-expressing plants. The authors found that sCAX1 + CRT-expressing tobacco and tomato exhibited lower Ca 2+ deficiency symptoms (leaf tip necrosis and BER) than the lines expressing sCAX1 did alone.…”

Section: Effects Of Genes Encoding Calreticulinmentioning

confidence: 99%

Identification of the Causative Genes of Calcium Deficiency Disorders in Horticulture Crops: A Systematic Review

Kuronuma

Watanabe

2021

Agriculture

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The occurrence of calcium (Ca2+) deficiency disorders is a severe problem in the production of horticulture crops. Recently, several studies have investigated the role of gene expression in Ca2+ deficiency disorders and/or Ca2+ accumulation, providing an indication of the mechanism of Ca2+ deficiency disorders at the genetic level. To determine the relation between gene expression and the occurrence of Ca2+ deficiency disorders, we conducted a systematic review of the literature using the Preferred Reporting Items for Systematic Reviews and Meta-analyses protocol. In our initial search, we extracted studies investigating the relationships between Ca2+ deficiency disorders (tipburn and blossom-end rot) and gene expression. In our second search, we extracted studies involving functional analyses of the genes associated with Ca2+ deficiency and/or Ca2+ accumulation in plant organs. Thirty-seven articles were extracted from both searches. Studies on Ca2+ movement-related genes (Ca2+ antiporters, calreticulin, Ca2+ pumps, Ca2+ channels, and pectin methylesterases) accounted for the majority of these articles. Particularly, the effects of the expression of CAXs (Ca2+/H+ antiporters) and CRT (calreticulin) on the occurrence of Ca2+ deficiency disorders were demonstrated in studies extracted from both searches. Further research focusing on these genes may reveal the causative genes for Ca deficiency disorders in different horticulture crops. We hope that the knowledge synthesized in this systematic review will contribute to the accumulation of further knowledge and elucidation of the causes of Ca2+ deficiency disorders.

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Ectopic expression of a maize calreticulin mitigates calcium deficiency-like disorders in sCAX1-expressing tobacco and tomato

Cited by 12 publications

References 49 publications

CAX1 Vacuolar Antiporter Overexpression in Potato Results in Calcium Deficiency in Leaves and Tubers by Sequestering Calcium as Calcium Oxalate

CAX1 Vacuolar Antiporter Overexpression in Potato Results in Calcium Deficiency in Leaves and Tubers by Sequestering Calcium as Calcium Oxalate

Modulating Plant Calcium for Better Nutrition and Stress Tolerance

Identification of the Causative Genes of Calcium Deficiency Disorders in Horticulture Crops: A Systematic Review

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