Ectopic expression of apple hexose transporter MdHT2.2 reduced the salt tolerance of tomato seedlings with decreased ROS-scavenging ability

Wang, Zhengyang; Liang, Yonghui; Jin, Yuru; Tong, Xiao-lei; Wei, Xiaoyu; Ma, Fengwang; Ma, Baiquan; Li, Mingjun

doi:10.1016/j.plaphy.2020.10.001

Cited by 12 publications

(12 citation statements)

References 47 publications

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“…However, overexpression of sugar/sugar alcohol transporters may also reduce the salt tolerance of plants. Ectopic expression of a fructose and glucose/H + symporter hexose transporter (MdHT2.2) of apple in tomato reduced ROS scavenging ability and thus reduced the salt tolerance of seedlings [ 1 ]. The result indicates that the intracellular distribution of sugar alcohols/sugars resulting from MdHT2.2 in tomatoes is not conducive to the elimination of intracellular ROS.…”

Section: Discussionmentioning

confidence: 99%

“…An increase in osmolytes correlates to a decrease in ROS production, thus improving tolerance to salt stress [ 38 , 39 ]. A large number of small molecules, such as proline and sugars/ sugar alcohols, will be produced to maintain cell osmotic potential in the plant in response to salt stress [ 1 , 4 , 40 ]. Here, OELjPLT3 plants accumulated more proline, trehalose, pinitol, and ononitol than the wild type after salt treatment ( Figure 3 ).…”

Section: Discussionmentioning

confidence: 99%

“…Soil salinity is a major agricultural problem, being one of the most damaging abiotic stresses. High salinity leads to hyperosmotic conditions, which impede the ability of a plant to absorb water and nutrients from the soil, impacting seed germination, root, and shoot development and even yield [ 1 ]. Legumes have the ability to form a symbiotic relationship with nitrogen-fixing rhizobial bacteria to form nodules, which is helpful in the improvement of barren soil [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of LjPLT3 Enhances Salt Tolerance in Lotus japonicus

Liu

Tian

et al. 2023

IJMS

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Intracellular polyols are used as osmoprotectants by many plants under environmental stress. However, few studies have shown the role of polyol transporters in the tolerance of plants to abiotic stresses. Here, we describe the expression characteristics and potential functions of Lotus japonicus polyol transporter LjPLT3 under salt stress. Using LjPLT3 promoter-reporter gene plants showed that LjPLT3 was expressed in the vascular tissue of L. japonicus leaf, stem, root, and nodule. The expression was also induced by NaCl treatment. Overexpression of LjPLT3 in L. japonicus modified the growth rate and saline tolerance of the transgenic plants. The OELjPLT3 seedlings displayed reduced plant height under both nitrogen-sufficient and symbiotic nitrogen fixation conditions when 4 weeks old. The nodule number of OELjPLT3 plants was reduced by 6.7–27.4% when 4 weeks old. After exposure to a NaCl treatment in Petri dishes for 10 days, OELjPLT3 seedlings had a higher chlorophyll concentration, fresh weight, and survival rate than those in the wild type. For symbiotic nitrogen fixation conditions, the decrease in nitrogenase activity of OELjPLT3 plants was slower than that of the wild type after salt treatment. Compared to the wild type, both the accumulation of small organic molecules and the activity of antioxidant enzymes were higher under salt stress. Considering the concentration of lower reactive oxygen species (ROS) in transgenic lines, we speculate that overexpression of LjPLT3 in L. japonicus might improve the ROS scavenging system to alleviate the oxidative damage caused by salt stress, thereby increasing plant salinity tolerance. Our results will direct the breeding of forage legumes in saline land and also provide an opportunity for the improvement of poor and saline soils.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Overexpression of LjPLT3 Enhances Salt Tolerance in Lotus japonicus

Liu

Tian

et al. 2023

IJMS

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“…The plant tissues accumulate high levels of soluble sugars, amino acids, glycine betaine, and proline intracellularly, as a means to cope with high salinity stress [ 6 ]. Moreover, reactive oxygen species (ROS) production and scavenging mechanisms, such as those involving superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), play a critical role in stress physiology [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Mechanisms of Tomato Seedlings Induced by Low-Red to Far-Red Light Ratio under Calcium Nitrate Stress

Zhou

Huang

You-min

et al. 2023

IJMS

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In recent times, the excessive accumulation of nitrate has been one of the main reasons for the secondary salinization of greenhouse soils. Light plays a key role in a plant’s growth, development, and response to stress. A low-red to far-red (R:FR) light ratio could enhance plant salinity tolerance, but the mechanism at a molecular level is unclear. Thus, we analyzed the transcriptome responses of tomato seedlings to calcium nitrate stress under either a low R:FR ratio (0.7) or normal light conditions. Under calcium nitrate stress, a low R:FR ratio enhanced both the antioxidant defense system and the rapid physiological accumulation of proline in tomato leaves, which promoted plant adaptability. Using weighted gene co-expression network analysis (WGCNA), three modules including 368 differentially expressed genes (DEGs) were determined to be significantly associated with these plant traits. Functional annotations showed that the responses of these DEGs to a low R:FR ratio under excessive nitrate stress were enriched in the areas of hormone signal transduction, amino acid biosynthesis, sulfide metabolism, and oxidoreductase activity. Furthermore, we identified important novel hub genes encoding certain proteins, including FBNs, SULTRs, and GATA-like transcription factor, which may play a vital role in low R:FR light-induced salt responses. These findings offer a new perspective on the mechanisms and environmental implications behind low R:FR ratio light-modulated tomato saline tolerance.

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“…AtSTP4, AtSTP6, AtSTP8, AtSTP9, AtSTP10 and AtSTP11 are mainly responsible for the uptake of glucose into the pollen tube of A. thaliana and play a role in the supply of monosaccharides during the growth of the pollen tube ( Rottmann et al., 2018 ). Heterologous expression of the apple hexose transporter gene MdHT2.2 in tomato promotes sucrose, fructose, and glucose accumulation ( Wang et al., 2020b ) in response to tomato salt tolerance mechanism by balancing cytoplasmic to intercellular ion concentrations and scavenging reactive oxygen species (ROS) ( Wang et al., 2020a ). In wheat, TaSTP3 is transcriptionally activated by the transcription factor TaWRKY19/61/82 during stripe rust, thereby increasing the sucrose concentration of host cells to guarantee carbon source supply for the fungus ( Huai et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

The functional analysis of sugar transporter proteins in sugar accumulation and pollen tube growth in pummelo (Citrus grandis)

Liu

Zhao

et al. 2023

Front. Plant Sci.

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Sugar transporter proteins (STPs) play vital roles in sugar transport and allocation of carbon sources in plants. However, the evolutionary dynamics of this important gene family and their functions are still largely unknown in citrus, which is the largest fruit crop in the world. In this study, fourteen non−redundant CgSTP family members were identified in pummelo (Citrus grandis). A comprehensive analysis based on the biochemical characteristics, the chromosomal location, the exon–intron structures and the evolutionary relationships demonstrated the conservation and the divergence of CgSTPs. Moreover, CgSTP4, 11, 13, 14 were proofed to be localized in plasma membrane and have glucose transport activity in yeast. The hexose content were significantly increased with the transient overexpression of CgSTP11 and CgSTP14. In addition, antisense repression of CgSTP4 induced the shorter pollen tube length in vitro, implying the potential role of CgSTP4 in pummelo pollen tube growth. Taken together, this work explored a framework for understanding the physiological role of CgSTPs and laid a foundation for future functional studies of these members in citrus species.

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Ectopic expression of apple hexose transporter MdHT2.2 reduced the salt tolerance of tomato seedlings with decreased ROS-scavenging ability

Cited by 12 publications

References 47 publications

Overexpression of LjPLT3 Enhances Salt Tolerance in Lotus japonicus

Overexpression of LjPLT3 Enhances Salt Tolerance in Lotus japonicus

Transcriptome Mechanisms of Tomato Seedlings Induced by Low-Red to Far-Red Light Ratio under Calcium Nitrate Stress

The functional analysis of sugar transporter proteins in sugar accumulation and pollen tube growth in pummelo (Citrus grandis)

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