Ectopic expression of <i>CYP24A1</i> circular RNA hsa_circ_0060927 in uterine leiomyomas

Fazeli, Elnaz; Piltan, Samira; Sadeghi, Hossein; Gholami, Milad; Azizi‐Tabesh, Ghasem; Yassaee, Fakhrolmolook; Mirfakhraie, Reza

doi:10.1002/jcla.23114

Cited by 5 publications

(5 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DEP domain containing 1 (DEPDC1) is a highly conserved protein in many species, from Cryptobacterium hidradenum to mammals (Kanehira et al, 2007). Guo et al (2020) showed that DEPDC1 exerts its oncogenic activity by suppressing CYP27B1 protein expression, while NNK promotes DEPDC1 upregulation by stimulating DNMT1 expression in OSCC. Similarly, DNA damage-inducible transcript 4 (DDIT4) and glutamic-oxaloacetic transaminase 1 (GOT1) as oncogenes are associated with poor prognosis in OSCC (Busso-Lopes et al, 2021;Han et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…APBB2 plays a dual regulatory role in bladder cancer, mediating the cell cycle through the CDK6 and MET pathways ( Li et al, 2019 ). CYP24A1 is a mitochondrial enzyme responsible for the inactivation of vitamin D. Fazeli et al (2020) demonstrated for the first time that hsa_circ_0060927 is ectopically expressed in uterine smooth muscle tumors compared to adjacent tissues.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Circular RNA-related CeRNA network and prognostic signature for patients with oral squamous cell carcinoma

Yang

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

Background: Circular RNA (circRNA) has an important influence on oral squamous cell carcinoma (OSCC) progression as competing endogenous RNAs (ceRNAs). However, the link between ceRNAs and the OSCC immune microenvironment is unknown. The research aimed to find circRNAs implicated in OSCC carcinogenesis and progression and build a circRNA-based ceRNA network to create a reliable OSCC risk prediction model.Methods: The expression profiles of circRNA in OSCC tumors and normal tissues were assessed through RNA sequencing. From the TCGA database, clinicopathological data and expression patterns of microRNAs (miRNAs) and mRNAs were obtained. A network of circRNA-miRNA-mRNA ceRNA was prepared according to these differentially expressed RNAs and was analyzed through functional enrichment. Subsequently, based on the mRNA in the ceRNA network, the influence of the model on prognosis was then evaluated using a risk prediction model. Finally, considering survival, tumor-infiltrating immune cells (TICs), clinicopathological features, immunosuppressive molecules, and chemotherapy efficacy were analyzed.Results: Eleven differentially expressed circRNAs were found in cancer tissues relative to healthy tissues. We established a network of circRNA-miRNA-mRNA ceRNA, and the ceRNA network includes 123 mRNAs, six miRNAs, and four circRNAs. By the assessment of Genomes pathway and Kyoto Encyclopedia of Genes, it is found that in the cellular senescence, PI3K-AKT and mTOR signaling pathway mRNAs were mainly enrichment. An immune-related signature was created utilizing seven immune-related genes in the ceRNA network after univariate and multivariate analysis. The receiver operating characteristic of the nomogram exhibited satisfactory accuracy and predictive potential. According to a Kaplan-Meier analysis, the high-risk group’s survival rate was signally lower than the group with low-risk. In addition, risk models were linked to clinicopathological characteristics, TICs, immune checkpoints, and antitumor drug susceptibility.Conclusion: The profiles of circRNAs expression of OSCC tissues differ significantly from normal tissues. Our study established a circRNA-associated ceRNA network associated with OSCC and identified essential prognostic genes. Furthermore, our proposed immune-based signature aims to help research OSCC etiology, prognostic marker screening, and immune response evaluation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Circular RNA-related CeRNA network and prognostic signature for patients with oral squamous cell carcinoma

Yang

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…The GO and KEGG pathways involved in hsa_circ_0060927 are summarized in Figure 4 . Cytochrome P450 family 24 subfamily A member 1 (CYP24A1), the source gene of hsa_circ_0060927, is located on chromosome 20 ( 23 ). GO analysis showed that, in terms of BP ( Figure 4A ), CYP24A1 was involved in “cellular regulation of organic matter metabolism and decomposition” (GO: 0044237, GO: 1901575), “trophic level cellular response” (GO: 0031669), and “response to extracellular stimuli” (GO: 0009991).…”

Section: Resultsmentioning

confidence: 99%

“…Circular RNA_LARP4, which could be used as a sponge to adsorb miR-424-5p and regulate LATS1 expression to inhibit the proliferation and invasion of gastric cancer cells, was also localized in the cytoplasm ( 34 ). It was reported that hsa_circ_0060927 may serve as a potential diagnostic biomarker for human colorectal and uterine leiomyomas ( 35 , 36 ). Based on the characteristics of circRNAs in the cytoplasm in literatures, hsa_circ_0060927 may have rich biological functions.…”

Section: Discussionmentioning

confidence: 99%

Hsa_circ_0060927 Is a Novel Tumor Biomarker by Sponging miR-195-5p in the Malignant Transformation of OLK to OSCC

Song

Shao

et al. 2022

Front. Oncol.

View full text Add to dashboard Cite

ObjectiveTo investigate the clinical significance of differentially expressed circRNAs and candidate circRNAs in the transformation of oral leukoplakia (OLK) to oral squamous cell carcinoma (OSCC).MethodsWe performed high-throughput circRNA sequencing in six cases of normal oral mucosal (NOM) tissues, six cases of OLK tissues, and six cases of OSCC tissues. Ten circRNAs with significant differential expression were verified by qRT-PCR. Enzyme tolerance assay and Sanger sequencing were performed on the screened target circRNA hsa_circ_0060927, and a qRT-PCR assay of hsa_circ_0060927 was performed in three tissues (24 cases in each group); this was followed by an ROC analysis. The ceRNA network was predicted using TargetScan and miRanda. MiR-195-5p and TRIM14 were selected as the downstream research objects of hsa_circ_0060927. The sponge mechanism of hsa_circ_0060927 was detected by AGO2 RIP. The interaction between hsa_circ_0060927 and miR-195-5p was verified by RNA pull-down assay and dual luciferase reporter gene assay. The expressions of hsa_circ_0060927, miR-195-5p, and TRIM14 were verified by normal oral epithelial primary cells and cell lines of LEUK1, SCC9, and SCC25. The hsa_circ_0060927 overexpressed plasmid and miR-195-5p mimics were constructed to transfection LEUK1 to detect the changes in cell proliferation, apoptosis, and migration.ResultsThe results of qRT-PCR validation were consistent with the sequencing results. Hsa_circ_0060927 is a true circRNA with trans-splicing sites. The expression of hsa_circ_0060927 increased in NOM, OLK, and OSCC. Overexpression of hsa_circ_0060927 enhanced the ability of cell proliferation and migration, and decreased cell apoptosis capacity. The prediction of ceRNA network suggested that hsa_circ_0060927 could regulate the target gene TRIM14 through sponging miR-195-5p. AGO2 RIP indicated that hsa_circ_0060927 had a sponge mechanism. RNA pull-down and dual luciferase reporter gene assay suggested that hsa_circ_0060927 interacted with miR-195-5p. Hsa_circ_0060927 was positively correlated with the expression of TRIM14, and could relieve the inhibition of miR-195-5p on TRIM14 to regulate cell proliferation, apoptosis, and migration of LEUK1 cells.ConclusionHsa_circ_0060927 acted as a potential key ceRNA to sponge downstream miR-195-5p and promote OLK carcinogenesis by upregulating TRIM14. Hsa_circ_0060927 was expected to be a molecular marker for the prevention and treatment of OLK carcinogenesis through the hsa_circ_0060927/miR-195-5p/TRIM14 axis.

show abstract

“…CYP24A1 is a mitochondrial enzyme, the main enzyme responsible for the degradation of active vitamin D, and has an important association with cancer (23,24). It has been reported that hsa_circ_0060927 is also abnormally expressed in uterine leiomyomas and colorectal cancer (25,26). The polymorphism of the CYP24A1 gene has been associated with the risk of OLP (27).…”

Section: Discussionmentioning

confidence: 99%

Expression profile of circular RNAs in oral lichen planus

Song¹,

Xu²,

Shao³

et al. 2021

Ann Palliat Med

View full text Add to dashboard Cite

Background: This study sought to identify the circular RNAs (circRNAs) differentially expressed in oral lichen planus (OLP) to investigate the possible role of circRNAs in this disease's pathogenesis.Methods: Six OLP and six normal oral mucosal tissues were used for circRNA detection and sequencing.10 selected circRNAs were verified by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). A gene ontology (GO) functional analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to predict the functions of circRNAs in OLP. TargetScan and miRanda were applied to predict targeted micro (mi)RNAs and messenger (m)RNAs of circRNAs, and competing endogenous (ce)RNA networks were mapped.Results: One hundred and thirty-five circRNAs were identified differentially expressed in OLP tissues compared to normal control tissues, including 83 upregulated circRNAs, and 52 down-regulated circRNAs.RT-qPCR confirmed that 10 circRNAs were all abnormally expressed in OLP. The GO functional analysis and KEGG pathway analysis showed that differentially expressed circRNAs were involved in 535 GO functional items and 78 signal pathways. A ceRNA network analysis showed that circRNAs might interact with a variety of miRNAs.Conclusions: This study mapped the expression profile of abnormally expressed circRNAs in OLP tissues for the first time and showed that circRNAs appear to play an important role in the pathogenesis of OLP.

show abstract

Ectopic expression of CYP24A1 circular RNA hsa_circ_0060927 in uterine leiomyomas

Cited by 5 publications

References 36 publications

Circular RNA-related CeRNA network and prognostic signature for patients with oral squamous cell carcinoma

Circular RNA-related CeRNA network and prognostic signature for patients with oral squamous cell carcinoma

Hsa_circ_0060927 Is a Novel Tumor Biomarker by Sponging miR-195-5p in the Malignant Transformation of OLK to OSCC

Expression profile of circular RNAs in oral lichen planus

Contact Info

Product

Resources

About