Ectopic expression of LoSVP, a MADS-domain transcription factor from lily, leads to delayed flowering in transgenic Arabidopsis

Tang, Xiaoli; Liang, Mao; Han, Juan; Cheng, Jieshan; Zhang, Hongxia; Liu, Xiaohua

doi:10.1007/s00299-019-02491-1

Cited by 14 publications

(3 citation statements)

References 32 publications

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“…Strawberry FaSVPs and kiwifruit AcSVPs are expressed only in vegetative organs and not in reproductive organs, which is consistent with the patterns in A. thaliana [ 24 , 25 , 26 ]. However, some different expression patterns have been found; for example, for Medicago MtSVP , Chrysanthemum morifolium CmSVP , grape lily LoSVP , Eriobotrya japonica EjSVPs , Prunus mume PmSVPSs , Malus × domestica MdSVPs , and another apple SVP -like gene, MdMADS50 , are all of highly expressed in vegetative tissues but weakly expressed in reproductive organs [ 23 , 27 , 28 , 29 , 30 , 31 , 32 ]. In this study, the qRT-PCR results suggested that the expression patterns of MiSVP1 and MiSVP2 were the same; however, the transcription levels of MiSVP1 and MiSVP2 were different.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Functional Characterization of Two SHORT VEGETATIVE PHASE Homologous Genes from Mango

Luo

et al. 2021

IJMS

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The SHORT VEGETATIVE PHASE (SVP) gene is a transcription factor that integrates flowering signals and plays an important role in the regulation of flowering time in many plants. In this study, two full-length cDNA sequences of SVP homologous genes—MiSVP1 and MiSVP2—were obtained from ‘SiJiMi’ mango. Sequence analysis showed that the MiSVPs had typical MADS-box domains and were highly conserved between each other. The analysis of expression patterns showed that the MiSVPs were expressed during flower development and highly expressed in vegetative tissues, with low expression in flowers/buds. The MiSVPs could responded to low temperature, NaCl, and PEG treatment. Subcellular localization revealed that MiSVP1 and MiSVP2 were localized in the nucleus. Transformation of Arabidopsis revealed that overexpression of MiSVP1 delayed flowering time, overexpression of MiSVP2 accelerated flowering time, and neither MiSVP1 nor MiSVP2 had an effect on the number of rosette leaves. Overexpression of MiSVP1 increased the expression of AtFLC and decreased the expression of AtFT and AtSOC1, and overexpression of MiSVP2 increased the expression levels of AtSOC1 and AtFT and decreased the expression levels of AtFLC. Point-to-point and bimolecular fluorescence complementation (BiFC) assays showed that MiSVP1 and MiSVP2 could interact with SEP1-1, SOC1D, and AP1-2. These results suggest that MiSVP1 and MiSVP2 may play a significant roles in the flowering process of mango.

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Section: Discussionmentioning

confidence: 99%

Isolation and Functional Characterization of Two SHORT VEGETATIVE PHASE Homologous Genes from Mango

Luo

et al. 2021

IJMS

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“…Plant flowering is an important life activity in the process of plants transitioning from vegetative growth to reproductive growth. Many genes are involved in the flowering process of plants, such as FLS (Park et al, 2020), MADS (Tang et al, 2020), and CDF (Corrales et al, 2014). Recent studies have shown that TCP genes also play a regulatory role in plant flowering (Lucero et al, 2017;Li et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomic Analysis of TCP Genes in Six Rosaceae Species and Expression Pattern Analysis in Pyrus bretschneideri

Zhao

Wang

et al. 2021

Front. Genet.

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TCP is a plant-specific transcription factor that plays an important role in flowering, leaf development and other physiological processes. In this study, we identified a total of 155 TCP genes: 34 in Pyrus bretschneideri, 19 in Fragaria vesca, 52 in Malus domestica, 19 in Prunus mume, 17 in Rubus occidentalis and 14 in Prunus avium. The evolutionary relationship of the TCP gene family was examined by constructing a phylogenetic tree, tracking gene duplication events, performing a sliding window analysis. The expression profile analysis and qRT-PCR results of different tissues showed that PbTCP10 were highly expressed in the flowers. These results indicated that PbTCP10 might participated in flowering induction in pear. Expression pattern analysis of different developmental stages showed that PbTCP14 and PbTCP15 were similar to the accumulation pattern of fruit lignin and the stone cell content. These two genes might participate in the thickening of the secondary wall during the formation of stone cells in pear. Subcellular localization showed that PbTCPs worked in the nucleus. This study explored the evolution of TCP genes in six Rosaceae species, and the expression pattern of TCP genes in different tissues of “Dangshan Su” pear. Candidate genes related to flower induction and stone cell formation were identified. In summary, our research provided an important theoretical basis for improving pear fruit quality and increasing fruit yield by molecular breeding.

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“…A significant delay of the onset of flowering was observed in thale cress transgenic plants expressing the MADS-box gene SHORT VEGETATIVE PHASE (LoSVP) from oriental lily (Tang et al 2020), lavander (Wells et al 1 3 2020) and chrysanthemum (CmSVP) (Gao et al 2017).…”

Section: Floral Meristem Initiation and Organ Developmentmentioning

confidence: 99%

Genes and genome editing tools for breeding desirable phenotypes in ornamentals

Giovannini¹,

Laura²,

Nesi

et al. 2021

Plant Cell Rep

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Key message We review the main genes underlying commercial traits in cut flower species and critically discuss the possibility to apply genome editing approaches to produce novel variation and phenotypes. Abstract Promoting flowering and flower longevity as well as creating novelty in flower structure, colour range and fragrances are major objectives of ornamental plant breeding. The novel genome editing techniques add new possibilities to study gene function and breed new varieties. The implementation of such techniques, however, relies on detailed information about structure and function of genomes and genes. Moreover, improved protocols for efficient delivery of editing reagents are required. Recent results of the application of genome editing techniques to elite ornamental crops are discussed in this review. Enabling technologies and genomic resources are reviewed in relation to the implementation of such approaches. Availability of the main gene sequences, underlying commercial traits and in vitro transformation protocols are provided for the world's best-selling cut flowers, namely rose, lily, chrysanthemum, lisianthus, tulip, gerbera, freesia, alstroemeria, carnation and hydrangea. Results obtained so far are described and their implications for the improvement of flowering, flower architecture, colour, scent and shelf-life are discussed. Keywords Ornamental crops • Genome editing • Plant regeneration • Plant transformation • Floriculture Abbreviations DSB Double-strand breaks NHEJ Nonhomologous end joining HDR Homology-directed repair CRISPR/Cas9 Clustered regularly interspaced short palindromic repeats/CRISPR-associated 9 endonuclease RNP Cas9-sgRNA ribonucleoprotein Electronic supplementary material The online version of this article (

show abstract

Ectopic expression of LoSVP, a MADS-domain transcription factor from lily, leads to delayed flowering in transgenic Arabidopsis

Cited by 14 publications

References 32 publications

Isolation and Functional Characterization of Two SHORT VEGETATIVE PHASE Homologous Genes from Mango

Isolation and Functional Characterization of Two SHORT VEGETATIVE PHASE Homologous Genes from Mango

Comparative Genomic Analysis of TCP Genes in Six Rosaceae Species and Expression Pattern Analysis in Pyrus bretschneideri

Genes and genome editing tools for breeding desirable phenotypes in ornamentals

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