Ectopic Expression of Sweet Potato Cysteine Protease SPCP3 Alters Phenotypic Traits and Enhances Drought Stress Sensitivity in Transgenic Arabidopsis Plants

Chen, Hsien-Jung; Tsai, Yi-Jing; Shen, Che-Yu; Tsai, Ting-No; Huang, Guan‐Jhong; Lin, Yi-Chun

doi:10.1007/s00344-012-9281-9

Cited by 20 publications

(8 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The overexpression of SPCP2 in Arabidopsis enhanced resistance to drought and salt stress (Chen et al, 2010). Whereas, the overexpression of sweet potato SPCP3 in Arabidopsis conferred sensitivity to drought stress (Chen et al, 2013). The expression of pepper ( Capsicum annuum ) PLCP gene ( CaCP ) was induced during leaf senescence and was also significantly unregulated by abiotic and biotic stress treatments (Xiao et al, 2014).…”

Section: Plcps Mediate Plant Abiotic Stress Responsementioning

confidence: 99%

Role of Papain-Like Cysteine Proteases in Plant Development

et al. 2018

View full text Add to dashboard Cite

Papain-like cysteine proteases (PLCP) are prominent peptidases found in most living organisms. In plants, PLCPs was divided into nine subgroups based on functional and structural characterization. They are key enzymes in protein proteolysis and involved in numerous physiological processes. In this paper, we reviewed the updated achievements of physiological roles of plant PLCPs in germination, development, senescence, immunity, and stress responses.

show abstract

Section: Plcps Mediate Plant Abiotic Stress Responsementioning

confidence: 99%

Role of Papain-Like Cysteine Proteases in Plant Development

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Most efforts to understand the role of CysProts under a drought conditions have been made by overexpressing genes ectopically (Chen et al, 2010(Chen et al, , 2013Zang et al, 2010). In our study, for the first time CysProt knock-down lines were generated in order to examine plant responses to drought.…”

Section: Discussionmentioning

confidence: 99%

“…with different growth patterns. Transgenic Arabidopsis plants constitutively overexpressing PSPC2 and SPCP3 CysProt from sweet potato present earlier floral transition from vegetative to reproductive growth, in addition to alterations in seed germination in comparison to WT plants (Chen et al, 2010(Chen et al, , 2013. On the other hand, HvPap-1 overexpressing barley lines exhibit a similar growth phenotype to that observed for WT plants (Velasco-Arroyo et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, the ectopic expression of the sweet potato CysProt (SPCP2) gene in Arabidopsis alters plant growth and enhances the tolerance to drought and/or salt stress (Chen et al, 2010). Conversely, CysProt may play the opposite role, as demonstrated by increased sensitivity to drought stress and altered phenotypic traits observed in transgenic Arabidopsis plants constitutively expressing the SPCP3 gene (Chen et al, 2013). C1A CysProt activities can be inhibited by a specific group of molecules termed cystatins (Benchabane et al, 2010;Martinez et al, 2012Martinez et al, , 2016.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Repression of drought-induced cysteine-protease genes alters barley leaf structure and responses to abiotic and biotic stresses

Gomez-Sanchez

González‐Melendi

Santamaría

et al. 2018

Journal of Experimental Botany

View full text Add to dashboard Cite

To survive under water deficiency, plants alter gene expression patterns, make structural and physiological adjustments, and optimize the use of water. Rapid degradation and turnover of proteins is required for effective nutrient recycling. Here, we examined the transcriptional responses of the C1A cysteine protease family to drought in barley and found that four genes were up-regulated in stressed plants. Knock-down lines for the protease-encoding genes HvPap-1 and HvPap-19 showed unexpected changes in leaf cuticle thickness and stomatal pore area. The efficiency of photosystem II and the total amount of proteins were almost unaltered in stressed transgenic plants while both parameters decreased in stressed wild-type plants. Although the patterns of proteolytic activities in the knock-down lines did not change, the amino acid accumulation increased in response to drought, concomitant with a higher ABA content. Whilst jasmonic acid (JA) and JA-Ile concentrations increased in stressed leaves of the wild-type and the HvPap-1 knock-down lines, their levels were lower in the HvPap-19 knock-down lines, suggesting the involvement of a specific hormone interaction in the process. Our data indicate that the changes in leaf cuticle thickness and stomatal pore area had advantageous effects on leaf defense against fungal infection and mite feeding mediated by Magnaporthe oryzae and Tetranychus urticae, respectively.

show abstract

“…Cysteine proteinases, also known as thiol or sulfhydryl proteinases, play various roles in plant growth and development, including senescence and programmed cell death (PCD) (Xu et al 2003 Ectopic expression of the cysteine protease SpCP3 from sweet potato in transgenic Arabidopsis enhanced the sensitivity of the plants to drought stress (Chen et al 2013). Heterologous expression of Salix matsudana SmCP in Arabidopsis improved salt tolerance, and this was associated with increased stability of cell membrane and activity of the antioxidant enzyme system (Zheng et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Heterologous expression of the cysteine proteinase gene VaCP17 from the cold-adapted grapevine Vitis amurensis increases cold tolerance in Arabidopsis

Shu

Zhang

et al. 2022

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Cysteine proteinases (thiol) carry out diverse and critical functions in plants through their ability to hydrolyze peptide bonds in target proteins. Here, we cloned a cysteine proteinase gene designated VaCP17 from a highly cold-resistant wild Vitis amurensis accession 'Shuangyou', and then its potential function in cold resistance was investigated. The results showed that the CDS of VaCP17 is 1404 bp, encoding 467 amino acids, the VaCP17 protein localized to the cell membrane. Expression of CP17 was highly distinctive among different structures of 'Shuangyou' and the cold-sensitive Vitis vinifera cultivar 'Red Globe', with the highest expression in the stem of 'Shuangyou' and the ower of 'Red Globe'. Arabidopsis plants constitutively expressing a VaCP17-GREEN FLUORESCENT PROTEIN fusion (35S::VaCP17-GFP) showed increased survival after transient exposure to freezing (-6℃), and showed lower electrolyte leakage and MDA content, higher soluble sugar content and SOD, POD and CAT activities, as compared with non-transgenic Arabidopsis controls. The expression of nine cold-resistance related genes (CBF1, CBF2, CBF3, RD29A, COR15A, KIN1, NCED3, AOC1 and JAZ10) in 35S::VaCP17-GFP plants was increased under cold treatment at 4℃, relative to control plants. Using a yeast two-hybrid system, we identi ed VaNAC72, VaCAM7 and VaDi19 as potential interactors of VaCP17, and their interactions were demonstrated by a bimolecular uorescence complementation assay. In conclusion, we revealed that VaCP17 can enhance cold resistance by in uencing physiology and biochemistry and the expression of cold resistance related genes under cold stress.

show abstract

Ectopic Expression of Sweet Potato Cysteine Protease SPCP3 Alters Phenotypic Traits and Enhances Drought Stress Sensitivity in Transgenic Arabidopsis Plants

Cited by 20 publications

References 40 publications

Role of Papain-Like Cysteine Proteases in Plant Development

Role of Papain-Like Cysteine Proteases in Plant Development

Repression of drought-induced cysteine-protease genes alters barley leaf structure and responses to abiotic and biotic stresses

Heterologous expression of the cysteine proteinase gene VaCP17 from the cold-adapted grapevine Vitis amurensis increases cold tolerance in Arabidopsis

Contact Info

Product

Resources

About