Ectopic Expression of the Allium cepa 1-SST Gene in Cotton Improves Drought Tolerance and Yield Under Drought Stress in the Field

Liu, Ruina; Jiao, TianQi; Zhang, ZeXing; Yao, Zhen; Li, ZhongQing; Wang, Saisai; Xin, Hongliang; Li, Yuxia; Wang, Aiying; Zhu, Jianbo

doi:10.3389/fpls.2021.783134

Cited by 7 publications

(4 citation statements)

References 71 publications

(81 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly to sucrose, fructan is an important storage carbohydrate that is also involved in stress responses, partly because it is soluble and osmotically active [ 35 , 36 ]. A previous study demonstrated that SST is a key enzyme for fructan biosynthesis in higher plants; the overexpression of SST genes improves the tolerance of cotton to drought [ 37 ] and the resistance of rice to low temperatures [ 38 ]. In the present study, the expression of genes encoding enzymes associated with carbon metabolism in the roots increased after exposure to LK stress, suggesting metabolic activities are enhanced to meet the increased energy demands for K + uptake and growth in wheat.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Roots Response to Potassium Deficiency and the Effect of TaHAK1-4A on K+ Uptake in Wheat

Zhao

et al. 2022

IJMS

View full text Add to dashboard Cite

Potassium (K+) is essential for plant growth and stress responses. A deficiency in soil K+ contents can result in decreased wheat quality and productivity. Thus, clarifying the molecular mechanism underlying wheat responses to low-K+ (LK) stress is critical. In this study, a tandem mass tag (TMT)-based quantitative proteomic analysis was performed to investigate the differentially abundant proteins (DAPs) in roots of the LK-tolerant wheat cultivar “KN9204” at the seedling stage after exposure to LK stress. A total of 104 DAPs were identified in the LK-treated roots. The DAPs related to carbohydrate and energy metabolism, transport, stress responses and defense, and post-translational modifications under LK conditions were highlighted. We identified a high-affinity potassium transporter (TaHAK1-4A) that was significantly up-regulated after the LK treatment. Additionally, TaHAK1-4A was mainly expressed in roots, and the encoded protein was localized in the plasma membrane. The complementation assay in yeast suggested that TaHAK1-4A mediates K+ uptake under extreme LK conditions. The overexpression of TaHAK1-4A increased the fresh weight and root length of Arabidopsis under LK conditions and improved the growth of Arabidopsis athak5 mutant seedlings, which grow poorly under LK conditions. Moreover, silencing of TaHAK1-4A in wheat roots treated with LK stress decreased the root length, dry weight, K+ concentration, and K+ influx. Accordingly, TaHAK1-4A is important for the uptake of K+ by roots exposed to LK stress. Our results reveal the protein metabolic changes in wheat induced by LK stress. Furthermore, we identified a candidate gene potentially relevant for developing wheat lines with increased K+ use efficiency.

show abstract

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Roots Response to Potassium Deficiency and the Effect of TaHAK1-4A on K+ Uptake in Wheat

Zhao

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…The most ideal breeding target genes for ADR are expected to improve yield under both drought and normal conditions. Several such genes have been identified, including TaPYL1‐1B (Mao et al, 2022), OsSDG708 (K. Chen et al, 2021), AtSINA2 (Yang et al, 2023), HaWRKY76 (Raineri et al, 2015), OsPYL9 (Usman et al, 2020) and Ac1‐SST (Liu et al, 2021).…”

Section: Concept Of Adrmentioning

confidence: 99%

“…Under well‐watered conditions, transgenic cotton yield was significantly increased compared with WT plants. For two consecutive years under drought conditions, transgenic plants increased yield per m 2 by an average of 20.9% compared with WT (Liu et al, 2021).…”

Section: Concept Of Adrmentioning

confidence: 99%

Potential breeding target genes for enhancing agronomic drought resistance: A yield‐survival balance perspective

Liu,

Ning,

Chen

et al. 2023

Plant Breeding

View full text Add to dashboard Cite

Amidst global climate warming, the urgency to enhance crop drought resistance has reached unprecedented levels. However, the achievement of superior drought‐resistant crop varieties, despite substantial research investments, remains constrained. This limited success in transitioning from the laboratory to the field can be partly attributed to the disparity between evaluating biological and agronomic drought resistance (ADR). ADR places emphasis on minimizing yield losses during drought conditions and maintaining robust performance under normal circumstances. Here, we present a comprehensive overview of ADR genes reported during the past decades, categorized based on their yield performance under both drought and standard growth conditions. We highlight 23 genes from grain and legume crops, providing insight into their working mechanisms. Particularly, we delve into their efficacy in improving yields predominantly through transgenic approaches in field conditions. Furthermore, we briefly touch upon the adoption of emerging phenomics technologies, which can streamline the discovery and application of ADR genes. This review is poised to serve the breeding community, aiding in the selection of appropriate target genes to augment crop drought resistance.

show abstract

“…Drought tolerance mechanisms in cotton include drought avoidance, drought tolerance, drought recovery, and drought escape [ 14 ]. These tolerance mechanisms are aided by signal transduction and hormone regulation, such as jasmonic acid (JA), ABA, and ethylene synthesis [ 11 , 13 , 14 , 20 , 26 , 32 , 34 , 52 , 56 ].…”

Section: Introductionmentioning

confidence: 99%

Silencing of GhORP_A02 enhances drought tolerance in Gossypium hirsutum

et al. 2023

View full text Add to dashboard Cite

Background ORP (Oxysterol-binding protein-related proteins) genes play a role in lipid metabolism, vesicular transferring and signaling, and non-vesicular sterol transport. However, no systematic identification and analysis of ORP genes have been reported in cotton. Result In this study, we identified 14, 14, 7, and 7 ORP genes in G. hirsutum, G. barbadense, G. arboreum, and G. raimondii, respectively. Phylogenetic analysis showed that all ORP genes could be classified into four groups. Gene structure and conserved motif analysis suggest that the function of this gene family was conserved. The Ka/Ks analysis showed that this gene family was exposed to purifying selection during evolution. Transcriptome data showed that four ORP genes, especially GhORP_A02, were induced by abiotic stress treatment. The cis-acting elements in the ORP promoters were responsive to phytohormones and various abiotic stresses. The silenced plants of GhORP_A02 were more sensitive to drought stress when compared to control. Conclusion The major finding of this study shed light on the potential role of ORP genes in abiotic stress and provided a fundamental resource for further analysis in cotton.

show abstract

Ectopic Expression of the Allium cepa 1-SST Gene in Cotton Improves Drought Tolerance and Yield Under Drought Stress in the Field

Cited by 7 publications

References 71 publications

Proteomic Analysis of Roots Response to Potassium Deficiency and the Effect of TaHAK1-4A on K+ Uptake in Wheat

Proteomic Analysis of Roots Response to Potassium Deficiency and the Effect of TaHAK1-4A on K+ Uptake in Wheat

Potential breeding target genes for enhancing agronomic drought resistance: A yield‐survival balance perspective

Silencing of GhORP_A02 enhances drought tolerance in Gossypium hirsutum

Contact Info

Product

Resources

About