Ectopic Mineralization: New Concepts in Etiology and Regulation

Giachelli, Cecilia M.

doi:10.1002/9783527619443.ch66

Cited by 1 publication

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“…First, calcium and phosphate, which readily form an insoluble precipitate, are present in virtually all tissues and body fluids; therefore, crystallization has to be actively inhibited. Second, the balance between phosphate and pyrophosphate is a crucial determinant in the regulation of this crystallization process ( Giachelli, 2008 ; Kirsch, 2012 ). Phosphate is an element that enables the formation of hydroxyapatite, whereas pyrophosphate is a strong chemical inhibitor of crystal formation ( Terkeltaub, 2001 ).…”

Section: Introductionmentioning

confidence: 99%

Pathological mineralization in a zebrafishenpp1mutant exhibits features of Generalized Arterial Calcification of Infancy (GACI) and Pseudoxanthoma Elasticum (PXE)

Apschner

Huitema

Bas

et al. 2014

Disease Models &Amp; Mechanisms

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In recent years it has become clear that, mechanistically, biomineralization is a process that has to be actively inhibited as a default state. This inhibition must be released in a rigidly controlled manner in order for mineralization to occur in skeletal elements and teeth. A central aspect of this concept is the tightly controlled balance between phosphate, a constituent of the biomineral hydroxyapatite, and pyrophosphate, a physiochemical inhibitor of mineralization. Here, we provide a detailed analysis of a zebrafish mutant, dragonfish (dgf), which is mutant for ectonucleoside pyrophosphatase/phosphodiesterase 1 (Enpp1), a protein that is crucial for supplying extracellular pyrophosphate. Generalized arterial calcification of infancy (GACI) is a fatal human disease, and the majority of cases are thought to be caused by mutations in ENPP1. Furthermore, some cases of pseudoxanthoma elasticum (PXE) have recently been linked to ENPP1. Similar to humans, we show here that zebrafish enpp1 mutants can develop ectopic calcifications in a variety of soft tissues – most notably in the skin, cartilage elements, the heart, intracranial space and the notochord sheet. Using transgenic reporter lines, we demonstrate that ectopic mineralizations in these tissues occur independently of the expression of typical osteoblast or cartilage markers. Intriguingly, we detect cells expressing the osteoclast markers Trap and CathepsinK at sites of ectopic calcification at time points when osteoclasts are not yet present in wild-type siblings. Treatment with the bisphosphonate etidronate rescues aspects of the dgf phenotype, and we detected deregulated expression of genes that are involved in phosphate homeostasis and mineralization, such as fgf23, npt2a, entpd5 and spp1 (also known as osteopontin). Employing a UAS-GalFF approach, we show that forced expression of enpp1 in blood vessels or the floorplate of mutant embryos is sufficient to rescue the notochord mineralization phenotype. This indicates that enpp1 can exert its function in tissues that are remote from its site of expression.

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Section: Introductionmentioning

confidence: 99%