Ectopic osteogenic capacity of freshly isolated adipose-derived stromal vascular fraction cells supported with platelet-rich plasma: A simulation of intraoperative procedure

Najman, Stevo; Cvetković, Vladimir J.; Najdanović, Jelena G.; Stojanović, Sanja; Vukelić-Nikolić, Marija; Vučković, Ivica; Petrović, Dragan

doi:10.1016/j.jcms.2016.08.011

Cited by 21 publications

(19 citation statements)

References 45 publications

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“…The great advantage of ADSCs compared to the other mesenchymal stem cells (MSCs) is that they can be isolated in large quantities from very abundant and easily accessible adipose tissue [3]. In addition to their ability to provide different cell types and possess regenerative potential [4,5,6,7], immunomodulatory activity of ADSCs, and MSCs in general, in vitro and in vivo has been reported that considerably enlarges the application field of these cells for the treatment of inflammatory diseases and autoimmune disorders [8,9,10,11]. MSCs can express immunomodulatory activity through direct contact with immune cells or through secretion products [12].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Conditioned Media of Stem Cells Derived from Lipoma and Adipose Tissue on Macrophages’ Response and Wound Healing in Indirect Co-culture System In Vitro

Stojanović

Najman

2019

IJMS

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Immunomodulatory and wound healing activities of adipose-derived stem cells (ADSCs) have been reported in various in vitro and in vivo experimental models suggesting their beneficial role in regenerative medicine and treatments of inflammatory-related disorders. Lipoma-derived stem cells (LDSCs) were reported as a potential tool in regenerative medicine due to the similarity with ADSCs but we have previously shown that LDSCs have different differentiation capacity than ADSCs despite a similar mesenchymal phenotype. To further analyze the potential differences and/or similarities between those two stem cell types, in the present study we examined the macrophages (MΦs)’ response, immunomodulatory and wound healing effect of conditioned media (CM) of LDSCs and ADSCs in indirect co-culture system in vitro. We confirmed similar mesenchymal phenotype and stemness state of LDSCs and ADSCs but indicated differences in expression of some inflammatory-related genes. Anti-inflammatory potential of CM of LDSCs and ADSCs, with pronounced effect of LDSCs, in unstimulated RAW 264.7 MΦs was evaluated by decrease in Tnf and increase in Il10 gene expression, which was confirmed by corresponding cytokines’ secretion analysis. Conditioned media of both LDSCs and ADSCs led to the functional activation of MΦs, with slightly more pronounced effect of CM of LDSCs, while both stimulated wound healing in vitro in a similar manner. Results of this study suggest that LDSCs secrete soluble factors like ADSCs and therefore may have a potential for application in regenerative medicine, due to immunomodulatory and wound healing activity, and indicate that LDSCs through secretome may interact with other cells in lipoma tissue.

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Section: Introductionmentioning

confidence: 99%

The Effect of Conditioned Media of Stem Cells Derived from Lipoma and Adipose Tissue on Macrophages’ Response and Wound Healing in Indirect Co-culture System In Vitro

Stojanović

Najman

2019

IJMS

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“…ADSCs can be isolated from abundant and easily accessible adipose tissue in large quantities with a minimal invasive procedure either from liposuction aspirates or adipose tissue biopsies [2] and have properties that make them good candidates for regenerative medicine applications [3]. The use of ADSCs in tissue engineering and regenerative medicine is very promising due to their self-renewal potential, proliferation capacity and great potential to differentiate into numerous cell types particularly adipocytes, osteoblasts, chondrocytes and endothelial cells [4,5,6,7]. It is reported that ADSCs can be successfully differentiated into the cells of all three germ lines which means that they can be considered as pluripotent and makes them a good candidate for wide range applications in the field of biomedical sciences [4].…”

Section: Introductionmentioning

confidence: 99%

Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature

Stojanović

Najman

Korać

2018

Cells

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Lipomas are benign adipose tissue tumors of unknown etiology, which can vary in size, number, body localization and cell populations within the tissue. Lipoma-derived stem cells (LDSCs) are proposed as a potential tool in regenerative medicine and tissue engineering due to their similar characteristics with adipose-derived stem cells (ADSCs) reported so far. Our study is among the first giving detailed insights into the molecular signature and differences in the differentiation capacity of LDSCs in vitro compared to ADSCs. Mesenchymal stem cell phenotype was analyzed by gene expression and flow cytometric analysis of stem cell markers. Adipogenesis and osteogenesis were analyzed by microscopic analysis, cytochemical and immunocytochemical staining, gene and protein expression analyses. We showed that both LDSCs and ADSCs were mesenchymal stem cells with similar phenotype and stemness state but different molecular basis for potential differentiation. Adipogenesis-related genes expression pattern and presence of more mature adipocytes in ADSCs than in LDSCs after 21 days of adipogenic differentiation, indicated that differentiation capacity of LDSCs was significantly lower compared to ADSCs. Analysis of osteogenesis-related markers after 16 days of osteogenic differentiation revealed that both types of cells had characteristic osteoblast-like phenotype, but were at different stages of osteogenesis. Differences observed between LDSCs and ADSCs are probably due to the distinct molecular signature and their commitment in the tissue that governs their different capacity and fate during adipogenic and osteogenic induction in vitro despite their similar mesenchymal phenotype.

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“…The 35 studies on SVF were conducted to treat bone (no of studies: 16) [17,[28][29][30][31][32][33][34][35][36][37][38][39][40][41][42], cartilage (no of studies: 13) [7,[43][44][45][46][47][48][49][50][51][52][53][54], and tendon (no of studies: 6) [55][56][57][58][59][60] defects (Figure 2). They were conducted in vitro [17,[28][29][30]43] and in vivo [7, 29, 31-39, 42-45, 55-58], and some of them were clinical studies [40-43, 46-54, 59, 60].…”

Section: Resultsmentioning

confidence: 99%

Stromal Vascular Fraction and Amniotic Epithelial Cells: Preclinical and Clinical Relevance in Musculoskeletal Regenerative Medicine

Veronesi

Maglio

Contartese

et al. 2021

Stem Cells International

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Musculoskeletal regenerative medicine is mainly based on the use of cell therapy to heal damaged tissues such as bone, cartilage, and tendons. Throughout the years, different cell types have been employed for the treatment of musculoskeletal diseases, in particular, mesenchymal stem cells (MSCs) derived from bone marrow (BMSCs) and adipose tissue (ADSCs). Though the results of these literature studies have been encouraging, there are some limitations, especially on long-term results. Recently, some interest has shifted towards new cell types such as the stromal vascular fraction (SVF) and amniotic endothelial cells (AECs). The aim of the present literature review is to evaluate preclinical and clinical studies that used SVF and AECs for musculoskeletal tissue regeneration. Forty-eight preclinical and clinical studies, performed in the last 10 years, were identified. Both SVF and AECs, injected or implanted with or without scaffolds, were shown to be valid alternatives, and in some ways superior, to ADSCs and BMSCs, being able to differentiate towards osteogenic, chondrogenic, and tenogenic lineages, and to promote cell and tissue regenerative potential. The use of SVF and AECs could represent a new regenerative treatment in several musculoskeletal pathologies, solving the problem of cell expansion in vitro.

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Ectopic osteogenic capacity of freshly isolated adipose-derived stromal vascular fraction cells supported with platelet-rich plasma: A simulation of intraoperative procedure

Cited by 21 publications

References 45 publications

The Effect of Conditioned Media of Stem Cells Derived from Lipoma and Adipose Tissue on Macrophages’ Response and Wound Healing in Indirect Co-culture System In Vitro

The Effect of Conditioned Media of Stem Cells Derived from Lipoma and Adipose Tissue on Macrophages’ Response and Wound Healing in Indirect Co-culture System In Vitro

Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature

Stromal Vascular Fraction and Amniotic Epithelial Cells: Preclinical and Clinical Relevance in Musculoskeletal Regenerative Medicine

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