Ectopic overexpression of AtmiR398b gene in tobacco influences seed germination and seedling growth

Feng, Xiaoming; Ye, Qiao; Mao, Ke

doi:10.1007/s11240-010-9704-x

Cited by 12 publications

(8 citation statements)

References 18 publications

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“…It would be logical to assume that this is due to the heightened activity of miR398 (CSDR-miRNA) under drought conditions in the tolerant rice cultivars. Over-expression of miR398 has been associated with increase in H 2 O 2 levels in tobbaco 87 , rice 88 and common bean 73 . The cultivar-specific decrease in the Cu/ZnSOD activity is also supported by a corresponding increase in the ROS levels in the tolerant cultivars.…”

Section: Discussionmentioning

confidence: 99%

Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22

Balyan

Kumar

Mutum

et al. 2017

Sci Rep

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Comparative characterization of microRNA-mediated stress regulatory networks in contrasting rice cultivars is critical to decipher plant stress response. Consequently, a multi-level comparative analysis, using sRNA sequencing, degradome analysis, enzymatic and metabolite assays and metal ion analysis, in drought tolerant and sensitive rice cultivars was conducted. The study identified a group of miRNAs “Cultivar-specific drought responsive” (CSDR)-miRNAs (osa-miR159f, osa-miR1871, osa-miR398b, osa-miR408-3p, osa-miR2878-5p, osa-miR528-5p and osa-miR397a) that were up-regulated in the flag-leaves of tolerant cultivar, Nagina 22 (N22) and Vandana, but down-regulated in the sensitive cultivar, Pusa Basmati 1 (PB1) and IR64, during drought. Interestingly, CSDR-miRNAs target several copper-protein coding transcripts like plantacyanins, laccases and Copper/Zinc superoxide dismutases (Cu/Zn SODs) and are themselves found to be similarly induced under simulated copper-starvation in both N22 and PB1. Transcription factor OsSPL9, implicated in Cu-homeostasis also interacted with osa-miR408-3p and osa-miR528-5p promoters. Further, N22 flag leaves showed lower SOD activity, accumulated ROS and had a higher stomata closure. Interestingly, compared to PB1, internal Cu levels significantly decreased in the N22 flag-leaves, during drought. Thus, the study identifies the unique drought mediated dynamism and interplay of Cu and ROS homeostasis, in the flag leaves of drought tolerant rice, wherein CSDR-miRNAs play a pivotal role.

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Section: Discussionmentioning

confidence: 99%

Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22

Balyan

Kumar

Mutum

et al. 2017

Sci Rep

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“…These two miRs have been postulated to participate in the regulation of seed germination, seedling growth and root length. Prior research showed that overexpression of miR398 and miR408 in transgenic plants inhibited seed germination, seedling growth and root length (Box et al, 2005, Feng et al, 2010. Therefore, the decrease in expression level of miR398 and miR408 in Au ENP-exposed seedlings was possibly responsible for the higher rate of seed germination, seedling growth, and root elongation compared to control seedlings observed.…”

Section: Mechanisms For Metallic Nanoparticle Effectsmentioning

confidence: 97%

The effects of metallic engineered nanoparticles upon plant systems: An analytic examination of scientific evidence

Tolaymat

Genaidy²,

Abdelraheem

et al. 2017

Science of The Total Environment

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Recent evidence for the effects of metallic engineered nanoparticles (ENPs) on plants and plant systems was examined together with its implications for other constituents of the Society-Environment-Economy (SEE) system. In this study, we were particularly interested to determine whether or not metallic ENPs have both stimulatory and inhibitory effects upon plant performance. An emphasis was made to analyze the scientific evidence on investigations examining both types of effects in the same studies. Analysis of evidence demonstrated that metallic ENPs have both stimulatory and inhibitory effects mostly in well-controlled environments and soilless media. Nano zero-valent iron (nZVI) and Cu ENPs have potential for use as micronutrients for plant systems, keeping in mind the proper formulation at the right dose for each type of ENP. The concentration levels for the stimulatory effects of Cu ENPs are lower than for those for nZVI. Newer findings showed that extremely smaller concentrations of Au ENPs (smaller than those for nZVI and Cu ENPs) induce positive effects for plant growth, which is attributed to effects on secondary metabolites. Ag ENPs have demonstrated their usage as antimicrobial/pesticidal agents for plant protection; however, precautions should be taken to avoid higher concentrations not only for plant systems, but also, other constituents in the SEE. Further research is warranted to investigate the stimulatory and inhibitory effects of metallic ENPs in soil media in order to broaden the horizon of sustainable agriculture production in terms of higher and safer yields so as to meet the food requirements of human population.

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“…Stress‐responsive genes including superoxide dismutases were identified as targets of miR398 in Arabidopsis thaliana (Bonnet et al ., ; Jones‐Rhoades and Bartel, ; Beauclair et al ., ). Ectopic overexpression of miR398 in tobacco ( Nicotiana tabacum L.) affects seed germination and seedling growth (Feng et al ., ), suggesting that expression of miR398 is strictly regulated in tobacco plants. As expected, expression of miR398 was faintly detectable in tobacco leaves but strongly induced in leaf‐derived callus cultured on sucrose‐containing medium (Figure a).…”

Section: Resultsmentioning

confidence: 99%

A removable virus vector suitable for plant genome editing

et al. 2017

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Plant genome editing is achieved by the expression of sequence-specific nucleases (SSNs). RNA virus vector-mediated expression of SSNs is a promising approach for transgene integration-free targeted mutagenesis in plants. However, the removal of virus vectors from infected plants is challenging because no antiviral drugs are available against plant viruses. Here, we developed a removable RNA virus vector that carries the target site of tobacco microRNA398 (miR398) whose expression is induced during shoot regeneration. In the inoculated leaves in which expression of miR398 is not induced, insertion of the miR398 target site did not affect the practicability of the virus vector. When shoots were regenerated from the infected leaves, miR398 was expressed and viral RNA was eliminated. The virus vector successfully expressed SSNs in inoculated leaves, from which virus-free genome-edited plants were regenerated via tissue culture.

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Ectopic overexpression of AtmiR398b gene in tobacco influences seed germination and seedling growth

Cited by 12 publications

References 18 publications

Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22

Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22

The effects of metallic engineered nanoparticles upon plant systems: An analytic examination of scientific evidence

A removable virus vector suitable for plant genome editing

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