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Abstract

ABSTRACT:The glucosamine-6-phosphate isomerase and Jacob glycoprotein gene expression levels were assessed during E. histolytica excystation from the feces of five patients. Excystation was performed at 0, 24, 48 and 96 h in Robinson medium, and RNA was extracted from the cysts and trophozoites. cDNA was synthesized to amplify the genes using real-time RT-PCR. The results demonstrated differences according to the incubation time and/or conversion of the cystic phase to the trophozoite, where the most important expression in the genes was observed at 96 h excystation. The expression levels of the genes studied positively correlated with the population of trophozoites during excystament.