Edible and medicinal fungi breeding techniques, a review: Current status and future prospects

Dong, Yifan; Miao, Renyun; Feng, Rencai; Wang, Tao; Yan, Junjie; Zhao, Xu; Han, Xing; Gan, Ying; Lin, Junbin; Li, Yujia; BingCheng, Gan; Zhao, Jin

doi:10.1016/j.crfs.2022.09.002

Cited by 15 publications

(5 citation statements)

References 125 publications

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“…Currently, the selection and breeding of elite strains with enhanced nutrients and health-promoting compounds is one of the main aims of the mushroom industry [ 44 ]. Germplasm resources provide the basic raw material and a reservoir of genes for breeding [ 45 ], so the characterization and evaluation of germplasm resources is essential for any breeding program, including edible mushrooms.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Evaluation of Erinacine A Contents in Mycelia of Hericium erinaceus Strains

Liu,

Song

et al. 2024

Foods

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Hericium erinaceus has long been favored for its remarkable nutritional and health-promoting benefits, and erinacine A is the key component responsible for the neuroprotective properties of H. erinaceus. Establishing an efficient method for separating erinacine A from H. erinaceus and screening the erinacine A-enriched strains is crucial to maximizing its benefits. Herein, we first reported that high-speed counter current chromatography (HSCCC) is an effective method for separating high-purity erinacine A. Using a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (4.5:5:4.5:5, v/v/v/v), erinacine A with a purity of over 95% was separated. Then, we evaluated the content and yield of erinacine A in the liquid-fermented mycelia of Hericium germplasms. Both the content and yield of erinacine A varied greatly among the surveyed strains. The significant effect of the strain on the erinacine A content and yield was revealed by an analysis of variance. The highest erinacine A content and yield were observed in the mycelia of a wild strain HeG, reaching 42.16 mg/g and 358.78 mg/L, which is superior to the current highest outcomes achieved using submerged cultivation. The isolation method established and the strains screened in this study can be beneficial for the scaling up of erinacine A extraction and nutraceutical development to industrial levels.

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Section: Resultsmentioning

confidence: 99%

Isolation and Evaluation of Erinacine A Contents in Mycelia of Hericium erinaceus Strains

Liu,

Song

et al. 2024

Foods

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“…Currently, genome editing techniques are used in the precision molecular breeding of mushrooms. This approach holds great promise for customizing mushroom strains with enhanced substrate conversion rates and improved environmental adaptability, thereby facilitating the advancement of circular agricultural economic practices ( Dong et al, 2022 ).…”

Section: Circular Economymentioning

confidence: 99%

Synthetic biology enables mushrooms to meet emerging sustainable challenges

Zou,

Li,

Mijakovic

et al. 2024

Front. Microbiol.

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With the increasing sustainability challenges, synthetic biology is offering new possibilities for addressing the emerging problems through the cultivation and fermentation of mushrooms. In this perspective, we aim to provide an overview on the research and applications mushroom synthetic biology, emphasizing the need for increased attention and inclusion of this rapidly advancing field in future mushroom technology over China and other countries. By leveraging synthetic biology, mushrooms are expected to play a more versatile role in various area, including traditional fields like circular economy, human wellness and pharmaceutics, as well as emerging fields like vegan meat, mushroom-based materials and pollution abatement. We are confident that these efforts using synthetic biology strategies have the potential to strengthen our capacity to effectively address sustainable challenges, leading to the development of a more sustainable social economy and ecology.

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“…In recent years, the edible mushroom industry has been continuously developing and scaling-up, leading to an increasing demand for new varieties [11]. However, due to the late start of research on P. giganteus and a lack of research capacities, increasing challenges have emerged such as unclear germplasm resources and a lack of DUS (distinctness, uniformity, and stability) test guidelines for variety evaluation [12].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Main Agronomic Traits of Different Pleurotus giganteus Germplasm Resources

Yan,

Zhai,

et al. 2024

Life

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Agronomic traits are key components in variety protection, cultivar development, and the formulation of DUS (distinct, uniform, and stable) test guidelines. P. giganteus is an increasingly popular and commercially promising edible macrofungi. In this study, both mycelial performance and fruiting body characters of 15 Pleurotus giganteus strains were investigated. The temperature gradient culture test indicated that, although most of the strains achieved optimal mycelial growth between 24 and 28 °C, a statistical difference in mycelial growth rates and temperature adaptability among strains were found, supporting that this trait has the potential to be adopted as an indicator in distinguishing strains. In the fruiting performance tests, the coefficient of variation (CV) of tested traits ranged from 5.30% (pileus diameter) to 18.70% (individual mushroom weight). The mushroom yields ranged from 103.37 g/bag (strain No. 15) to 275.76 g/bag (strain No. 9). The large divergence observed in individual mushroom weight tested strains, ranging from 40.88 g to 78.39 g (with median between 37.69 and 79.395 g), make it highly selective and a potential indicator in variety development. Strain No. 9 had the advantages of forming larger, heavier fruiting bodies and a more obvious funnel shape, which also exhibited the highest biological efficiency (15.61%). The results suggested some morphological traits showed high variety difference, such as pileus diameter (55.75 mm to 66.48 mm), stipe length (92.59 mm to 177.51 mm), stipe diameter (16.14 mm to 23.52 mm), and pileus thickness (13.38 mm to 19.75 mm). In the cluster analysis, the tested strains were grouped into four clusters based on agronomic traits: cluster Ⅰ comprised six strains (No. 6, No. 11, No. 8, No. 1, No. 14, and No. 9) with high mushroom yield; cluster Ⅱ included four strains (No. 3, No. 10, No. 7, and No. 4) with large pileus diameter and short stipe; cluster ⅡI consisted of four strains (No. 5, No. 12, No. 13, and No. 15) with relatively lower yields; and cluster Ⅳ included only strain No. 2 which was low in yield, individual mushroom weight, and biological efficiency, accompanied by smaller pileus size and shorter stipe. The results of the correlation analysis indicated three traits, including individual mushroom weight, stipe length, and pileus weight, were positively associated with high yield. This study suggested P. giganteus germplasm resources are of high abundance and their agronomic diversity is useful in distinguishing and developing different varieties. The findings of this work provide knowledge on the agronomic traits and cultivation performance of various P. giganteus strains, laying a foundation for the development of its DUS test guidelines and variety protection, as well as providing reference for the breeding and phenotype selection of high-quality cultivars.

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Edible and medicinal fungi breeding techniques, a review: Current status and future prospects

Cited by 15 publications

References 125 publications

Isolation and Evaluation of Erinacine A Contents in Mycelia of Hericium erinaceus Strains

Isolation and Evaluation of Erinacine A Contents in Mycelia of Hericium erinaceus Strains

Synthetic biology enables mushrooms to meet emerging sustainable challenges

Comparative Analysis of Main Agronomic Traits of Different Pleurotus giganteus Germplasm Resources

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