Sound waves are vibrations that propagate through a medium. Sound waves can be applied to agricultural land as a technology that is often called Plant Acoustic Frequency Technology (PAFT). This technology is applied to agricultural land by using sound that has been manipulated with a certain frequency. This study aims to provide the sound effect of Dundubia Manifera to determine the effect on stomatal density and stomatal index. This sound has been manipulated using Adobe Audition CS6 to be 4000 Hz which will be applied 30 minutes for 28 days. The results show that stomatal density and stomata index are affected by exposure to sound waves. In which, stomatal density was reduced by 89% with control plants (97,506 /mm2) and treatment plants (51,428 /mm2). Meanwhile, the stomatal index increased by 41% with control plants (0.194 m) and treatment plants (0.274 m). These results indicate that stomata have a very good impact on plants such as increasing the rate of photosynthesis, plant immune systems, surviving climate change, and increasing crop yields.