The Czech Republic is one of the smaller states in Central Europe with about 10 million inhabitants. In the frame of problematic of regional development and regional planning issues interregional differences are solved. With regard to the fact Czech Republic is a relatively small country; the conception of regional development by the principle of subsidiarity is transmitted to the level of municipalities. One of the monitored effects, which influence sustainable development, is also the existence of underused land or also previously used land (brownfields). Non-governmental or non-profit institutions may participate in the regeneration or re-using of these areas. The consequences of existence of these areas in concrete municipality are analyzed. The possibility of how these process regenerations were solved in the Czech Republic are indicated in the frame of this article on the example of several localities indicated. An important part of the contribution is devoted to effects which this successful regeneration brought to their surroundings. Examples were deliberately chosen not with investments having the expectation of future profit but with inputs of small financial funds and big enthusiasm which achieved significant regional effects.