Editorial: Advances and Challenges of RNAi Based Technologies for Plants—Volume 2

Mezzetti, Bruno; Arpaia, Salvatore; Baraldi, Elena; Dietz‐Pfeilstetter, Antje; Smagghe, Guy; Ventura, Vera; Sweet, Jeremy

doi:10.3389/fpls.2022.930851

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…However, additional research is needed before widespread use of such commercial products in forestry. To ensure its safety, a combination of bioinformatics and ecologically sound bioassays with selected focal insect species will help a thorough understanding of potential off-target effects and impacts non-target organisms (Christiaens et al 2022;Mezzetti et al 2022). These products must be used only to maintain the bark beetle populations under endemic conditions and preserve the beneficial role of bark beetles as decomposers in forest ecosystems.…”

Section: Rna Interference (Rnai)mentioning

confidence: 99%

Understanding bark beetle outbreaks: exploring the impact of changing temperature regimes, droughts, forest structure, and prospects for future forest pest management

Singh,

Naseer,

Mogilicherla

et al. 2024

Rev Environ Sci Biotechnol

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Climate change has increased the susceptibility of forest ecosystems, resulting in escalated forest decline globally. As one of the largest forest biomasses in the Northern Hemisphere, the Eurasian boreal forests are subjected to frequent drought, windthrow, and high-temperature disturbances. Over the last century, bark beetle outbreaks have emerged as a major biotic threat to these forests, resulting in extensive tree mortality. Despite implementing various management strategies to mitigate the bark beetle populations and reduce tree mortality, none have been effective. Moreover, altered disturbance regimes due to changing climate have facilitated the success of bark beetle attacks with shorter and multivoltine life cycles, consequently inciting more frequent bark beetle-caused tree mortality. This review explores bark beetle population dynamics in the context of climate change, forest stand dynamics, and various forest management strategies. Additionally, it examines recent advancements like remote sensing and canine detection of infested trees and focuses on cutting-edge molecular approaches including RNAi-nanoparticle complexes, RNAi-symbiotic microbes, sterile insect technique, and CRISPR/Cas9-based methods. These diverse novel strategies have the potential to effectively address the challenges associated with managing bark beetles and improving forest health in response to the changing climate.

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Section: Rna Interference (Rnai)mentioning

confidence: 99%

Understanding bark beetle outbreaks: exploring the impact of changing temperature regimes, droughts, forest structure, and prospects for future forest pest management

Singh,

Naseer,

Mogilicherla

et al. 2024

Rev Environ Sci Biotechnol

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show abstract

“…In addition, both conserved and species-specific miRNAs may play important roles in plant responses to abiotic stress, and, for this reason, genetically improving plant tolerance to GCC may be possible through the application of miRNA technology. Currently, the most popular application of miRNAs and RNA interference (RNAi) is host-induced gene silencing (HIGS), which essentially focuses on the control of insect pests and viral plant diseases [62]. The use of these approaches has been restricted to genetic transformation procedures and the production of genetically modified plants.…”

Section: Future Perspectivesmentioning

confidence: 99%

Small RNA Differential Expression Analysis Reveals miRNAs Involved in Dormancy Progression in Sweet Cherry Floral Buds

Soto

Sanchez

Núñez

et al. 2022

Plants

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In sweet cherry (Prunus avium), as in other temperate woody perennials, bud dormancy allows for survival in adverse environmental conditions during winter. During this process, environmental signals such as short days and/or low temperatures trigger internal signals that enable buds to become tolerant to the cold. The process involves tracking chilling units up to chilling the requirement fulfillment to resume growth, a transition involving transcriptional regulation, metabolic signaling, and epigenetic-related regulatory events. Massive sequencing of small RNAs was performed to identify miRNAs involved in sweet cherry dormancy by comparing their expression in field (regular seasonal) and controlled non-stop (continuous) chilling conditions. miRNAs highlighted by sequencing were validated using specific stem-loop PCR quantification, confirming expression patterns for known miRNAs such as miR156e, miR166c, miR172d, miR391, miR482c, and miR535b, as well as for newly proposed miRNAs. In silico prediction of the target genes was used to construct miRNA/target gene nodes. In particular, the involvement of the sweet cherry version for the miR156/SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN genes whose expression was opposite in the two conditions suggests their involvement on dormancy regulation in sweet cherry. miRNA levels indicate that the regulation of stress-related genes and hormone synthesis modulates the expression of calcium metabolism and cell development-associated genes. Understanding the regulatory networks involved in sweet cherry dormancy, particularly in the context of miRNA involvement, represents the first step in the development of new agricultural strategies that may help overcome the increasing challenges presented by global climate change.

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Editorial: Advances and Challenges of RNAi Based Technologies for Plants—Volume 2

Cited by 2 publications

References 24 publications

Understanding bark beetle outbreaks: exploring the impact of changing temperature regimes, droughts, forest structure, and prospects for future forest pest management

Understanding bark beetle outbreaks: exploring the impact of changing temperature regimes, droughts, forest structure, and prospects for future forest pest management

Small RNA Differential Expression Analysis Reveals miRNAs Involved in Dormancy Progression in Sweet Cherry Floral Buds

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