Editorial: Advances in Pollen Research: Biology, Biotechnology, and Plant Breeding Applications

Gómez‐Mena, Concepción; Honys, David; Datla, Raju; Testillano, P. S.

doi:10.3389/fpls.2022.876502

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…(Rafiq et al, 2021). At present, most scholars believe that in vitro pollen germination is the most common and simplest method and can be applied to study many basic and applied aspects of pollen biology (Gomez-Mena et al, 2022). This technique is more suitable for detecting viable and potential pollen form cross-pollinated plants (Schueler et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Influence of Sucrose, Boric Acid and Calcium Chloride on in vitro Pollen Germination and Tube Elongation of Alfalfa

Shi¹,

Shi²,

Wang³

2023

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Background: In a genetic breeding program, understanding the viability of pollen germination and pollen tube growth is an essential factor for artificial hybridization. Currently, little is known about the influence of sucrose, boric acid concentrations and calcium chloride on pollen viability in alfalfa. This study is valuable for providing information about pollen germination requirements to support further improvement of artificial pollination and breeding programs in alfalfa. Methods: The single-factor experimental and an orthogonal design method (L16 [4]3) was used to compare the effectiveness of key factors (e.g., concentrations of sucrose, boric acid and calcium chloride) to determine the pollen vigor of Medicago varia Martin. ‘Caoyuan No.1’and M. ruthenica L. Trautv. ‘zhilixing’ and to optimize the culture medium for in vitro pollen germination. Four different varieties were chosen to compare the diurnal variation of pollen vigor. Result: The four factors that influenced pollen germination in Medicago varia Martin. ‘Caoyuan No.1’and M. ruthenica L. Trautv. ‘zhilixing’ in order of significance are as follows: sucrose greater than H3BO3 greater than CaCl2. These variables influenced pollen tube growth in the following order: sucrose greater than H3BO3 greater than CaCl2. The optimal culture medium for promoting pollen vigor of Medicago varia Martin.‘Caoyuan No.1’ was 10% sucrose, 300 mon/L H3BO3 and 100 mon/L CaCl2; that of M. ruthenica L. Trautv. ‘zhilixing’ was 10% sucrose, 200 mon/L H3BO3 and 50 mon/L CaCl2. Among several species of alfalfa, ‘Caoyuan No.1’ shows the highest pollen vigor and the daily pollen activity curves of all varieties showed a single peak type.

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Section: Introductionmentioning

confidence: 99%

Influence of Sucrose, Boric Acid and Calcium Chloride on in vitro Pollen Germination and Tube Elongation of Alfalfa

Shi¹,

Shi²,

Wang³

2023

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“…Of course, the most accurate method is to pollinate the stigma and count the tubes under the microscope after staining them. With this in mind and considering the importance of pollen viability and germination for plant fertilization, an in vitro method to assess this potential over time would be very useful, especially for food plants (Gómez-Mena et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Nanoporous silica gel can compete with the flower stigma in germinating and attracting pollen tubes

2022

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To find nanoporous substrates with hydrodynamic properties useful for pollen hydration and germination, we used the glassy Silica gel and Vycor scales and pollen with different morphological and physiological traits, that of Crocus vernus, and that of Narcissus poeticus. For in vitro tests, the scales were spread on microscope slides, hand pollinated, and incubated. Pollen germination was evaluated with the stereomicroscope and the tube growth was explored with scanning electron microscopy (SEM). The in vivo tests were carried out by sprinkling the stigmas of the Crocus plants with Silica gel scales and immediately after having pollinated them by hand, the plants were incubated. Three hours later, the stigmas were removed and treated for observation with SEM. In vitro the pollen of both species germinated on Silica gel with percentages similar to those of the in vivo and in vitro controls, accumulating fibrillary material at the interface. The tubes grew perpendicular to the surface of the scales, trying to penetrate the scales to the point of flattening with the apex. On Crocus stigmas sprinkled with Silica gel scales, pollen developed tubes that grew to the scales rather than penetrating the papillae. The results underline the close interaction of pollen with nanoporous artificial material, so much so that its pollen tubes are attracted to the Silica scales more than to the stigma papillae that arises from a mechanism of natural selection.

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Anther development in Brachiaria brizantha (syn. Urochloa brizantha) and perspective for microspore in vitro culture

et al. 2022

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Editorial: Advances in Pollen Research: Biology, Biotechnology, and Plant Breeding Applications

Cited by 3 publications

References 5 publications

Influence of Sucrose, Boric Acid and Calcium Chloride on in vitro Pollen Germination and Tube Elongation of Alfalfa

Influence of Sucrose, Boric Acid and Calcium Chloride on in vitro Pollen Germination and Tube Elongation of Alfalfa

Nanoporous silica gel can compete with the flower stigma in germinating and attracting pollen tubes

Anther development in Brachiaria brizantha (syn. Urochloa brizantha) and perspective for microspore in vitro culture

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