Editorial: Cryopreservation of mammalian gametes and embryos: implications of oxidative and nitrosative stress and potential role of antioxidants

Ofosu, Jones; Zhang, Yunhai; Liu, Ying; Sun, Xiaofang; Quan, Guobo; Rodriguez, Manuel Alvarez; Zhou, Guangbin

doi:10.3389/fvets.2023.1174756

Cited by 4 publications

(2 citation statements)

References 35 publications

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“…Antioxidants can act as free radical scavengers and, therefore, protect cells against potential damage by repairing damage caused by ROS and RNS (reactive nitrogen species) [8]. Exogenous and endogenous antioxidants can offset extreme ROS concentrations generated from vitrification/warming and improve embryo quality [9]. However, the endogenous antioxidant system is insufficient in vitro; therefore, adding exogenous antioxidants could be an option to improve in vitro culture conditions [10].…”

Section: Introductionmentioning

confidence: 99%

Astaxanthin Added during Post-Warm Recovery Mitigated Oxidative Stress in Bovine Vitrified Oocytes and Improved Quality of Resulting Blastocysts

Dujíčková,

Olexiková,

Makarevich

et al. 2024

Antioxidants

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Various antioxidants are tested to improve the viability and development of cryopreserved oocytes, due to their known positive health effects. The aim of this study was to find whether astaxanthin (AX), a xanthophyll carotenoid, could mitigate deteriorations that occurred during the vitrification/warming process in bovine oocytes. Astaxanthin (2.5 µM) was added to the maturation medium during the post-warm recovery period of vitrified oocytes for 3 h. Afterward, the oocytes were fertilized in vitro using frozen bull semen and presumptive zygotes were cultured in the B2 Menezo medium in a co-culture with BRL-1 cells at 38.5 °C and 5% CO2 until the blastocyst stage. AX addition significantly reduced ROS formation, lipid peroxidation, and lysosomal activity, while increasing mitochondrial activity in vitrified oocytes. Although the effect of AX on embryo development was not observed, it stimulated cell proliferation in the blastocysts derived from vitrified oocytes and improved their quality by upregulation or downregulation of some genes related to apoptosis (BCL2, CAS9), oxidative stress (GPX4, CDX2), and development (GJB5) compared to the vitrified group without AX. Therefore, the antioxidant properties of astaxanthin even during short exposure to bovine vitrified/warmed oocytes resulted in improved blastocyst quality comparable to those from fresh oocytes.

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Section: Introductionmentioning

confidence: 99%

Astaxanthin Added during Post-Warm Recovery Mitigated Oxidative Stress in Bovine Vitrified Oocytes and Improved Quality of Resulting Blastocysts

Dujíčková,

Olexiková,

Makarevich

et al. 2024

Antioxidants

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“…Oxidative stress, one of the crucial contributing factors to spermatozoa cryo-injuries, is a result of excessive intracellular reactive oxygen species (ROS) formation [ 6 ]. The main organelles of ROS formation in spermatozoa are the mitochondria [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Post-Thaw Parameters of Buck Semen Quality after Soy Lecithin Extender Supplementation with Fumaric Acid

Saratsi,

Samartzi,

Panagiotidis

et al. 2023

Veterinary Sciences

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The supplementation of cryopreservation media with antioxidants improves the post-thaw quality and fertilizing ability of spermatozoa. To maximize the fertility of frozen–thawed buck spermatozoa, further research is required to overcome obstacles that have yielded controversial results and standardize protocols. In the present work, the effect of adding fumaric acid (a well-described antioxidant) to a soy lecithin semen extender on certain quality parameters of spermatozoa following freezing and thawing was examined for the first time. Five sexually mature Skopelos bucks were used, and ejaculates were collected with an artificial vagina. The semen samples (98 samples, five replicates) were diluted (400 × 106 spermatozoa/mL) with OviXcell®, supplemented with fumaric acid (0 mM, 2.15 mM, 10 mM or 30 mM), equilibrated (5 °C; 3 h), packed (0.5 mL straws), frozen and stored (−196 °C) until further processing. After thawing, the spermatozoa total and progressive motility (CASA), viability (eosin–nigrosin), membrane functional integrity (HOST), acrosome integrity (SpermBlue®) and mitochondrial function (Rhodamine-123/SYBR-14/PI) were evaluated. Statistical analysis was performed with one-way ANOVA, followed by Duncan’s test; significance was set at 0.05. The addition of 2.15 mM fumaric acid improved (p < 0.05) spermatozoa viability, membrane functional integrity, acrosome integrity and mitochondrial function compared to all other concentrations. The addition of 30 mM fumaric acid decreased (p < 0.05) spermatozoa viability and mitochondrial function compared to all other concentrations. These results indicate a beneficial effect of a 2.15 mM fumaric acid addition to a soy lecithin extender on post-thaw buck spermatozoa quality. Further research is required to evaluate the in vivo fertility of frozen–thawed buck spermatozoa treated with fumaric acid, as well as to elucidate the mechanism of action of fumaric acid in spermatozoa.

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Factors affecting cryotolerance of mammalian oocytes

Olexiková,

Makarevich,

Dujíčková

et al. 2024

Cryobiology

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Editorial: Cryopreservation of mammalian gametes and embryos: implications of oxidative and nitrosative stress and potential role of antioxidants

Cited by 4 publications

References 35 publications

Astaxanthin Added during Post-Warm Recovery Mitigated Oxidative Stress in Bovine Vitrified Oocytes and Improved Quality of Resulting Blastocysts

Astaxanthin Added during Post-Warm Recovery Mitigated Oxidative Stress in Bovine Vitrified Oocytes and Improved Quality of Resulting Blastocysts

Post-Thaw Parameters of Buck Semen Quality after Soy Lecithin Extender Supplementation with Fumaric Acid

Factors affecting cryotolerance of mammalian oocytes

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