Editorial: Current advancements in real-time plant pathogen diagnostics: from lab assays to in-field detection

Kumar, Ravinder; Lal, Milan Kumar; Prasad, Pramod; Tiwari, Rahul Kumar

doi:10.3389/fpls.2023.1255654

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…The Magnetozyme biosensor we developed competes favorably with existing plant virus diagnostics, offering an affordable, equipment-free, and practical tool for field use. It is the first of its kind among all plant disease diagnostic tools. − Paper-based biosensors like this can be operated anywhere with ease, providing qualitative and quantitative results. These nanoparticle-enhanced sensors offer a cost-effective alternative for sugarcane virus diagnosis, with a sensitivity that enables detection of low viral titer.…”

Section: Discussionmentioning

confidence: 99%

Magnetic Nanozyme-Enhanced Rapid ImmunoFlow-Through Assay for the Femtomolar Detection of Sugarcane Yellow Leaf Virus

Thangavelu,

PK,

Ramasamy

et al. 2024

ACS Agric. Sci. Technol.

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Plant diseases significantly threaten global food security, with numerous historical instances of devastating epidemics. This risk is particularly acute in key agricultural and food crops, such as sugarcane. Although recent advancements in molecular diagnostics have improved the detection of sugarcane viruses, these methods are largely confined to lab settings due to their reliance on sophisticated, costly equipment. To overcome this limitation, we have developed a more accessible and cost-effective solution: a magnetic nanozyme-enhanced colorimetric ImmunoFlow-through assay designed for the ultrasensitive detection of sugarcane yellow leaf curl virus (ScYLV). This innovative technique allows for clear optical identification of viral concentrations as low as femtomolar levels. The assay employs cationic magnetic nanoparticles for virus isolation and colorimetric immunolabels for diagnosis, enhancing sensitivity and providing immediate results, comparable to those of established methods like quantitative real-time-polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA). Our assay offers a one-step detection process and a two-step semiquantitative analysis, marking a major breakthrough in plant virus diagnostics. Extensive research into the assay’s design, including its sensing platform, blocking agents, antibody conjugation chemistries, sensitivity, quantification, potential for multiplexing, and field applicability, was carried out. This diagnostic research utilizing Magnetozyme in a flow-through assay represents a pioneering approach to rapid and sensitive diagnosis within plant disease diagnostics. It introduces a promising alternative to traditional molecular diagnostics, potentially transforming plant disease management and enhancing food security globally.

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Section: Discussionmentioning

confidence: 99%

Magnetic Nanozyme-Enhanced Rapid ImmunoFlow-Through Assay for the Femtomolar Detection of Sugarcane Yellow Leaf Virus

Thangavelu,

PK,

Ramasamy

et al. 2024

ACS Agric. Sci. Technol.

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“…Plant pathogens are ubiquitous in nature and have enormous effects in agriculture, where they cause approximately USD 220 billion of losses to the global economy each year [1]. The most obvious consequence of pathogen infection is the presence of disease symptoms on the plant.…”

Section: Introductionmentioning

confidence: 99%

Does This Look Infected? Hidden Host Plant Infection by the Pathogen Botrytis cinerea Alters Interactions between Plants, Aphids and Their Natural Enemies in the Field

Ngah,

Thomas,

Fellowes

2024

Insects

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Few studies have considered whether hidden (asymptomatic) plant pathogen infection alters ecological interactions at the higher trophic levels, even though such infection still affects plant physiology. We explored this question in two field experiments, where two varieties of lettuce (Little Gem, Tom Thumb) infected with Botrytis cinerea were either (1) naturally colonised by aphids or (2) placed in the field with an established aphid colony. We then recorded plant traits and the numbers and species of aphids, their predators, parasitoids and hyperparasitoids. Infection significantly affected plant quality. In the first experiment, symptomatically infected plants had the fewest aphids and natural enemies of aphids. The diversity and abundance of aphids did not differ between asymptomatically infected and uninfected Little Gem plants, but infection affected the aphid assemblage for Tom Thumb plants. Aphids on asymptomatically infected plants were less attractive to predators and parasitoids than those on uninfected plants, while hyperparasitoids were not affected. In the second experiment, when we excluded natural enemies, aphid numbers were lower on asymptomatically and symptomatically infected plants, but when aphid natural enemies were present, this difference was removed, most likely because aphids on uninfected plants attracted more insect natural enemies. This suggests that hidden pathogen infection may have important consequences for multitrophic interactions.

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Assessing Olive Oil Quality Using Different DNA-Based Methods

Moscato,

Bonavita,

Regina

2024

Plants

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Olive oil is appreciated worldwide for its unique nutritional and organoleptic properties. It is rich in unsaturated fatty acids and antioxidants, which are well-known for their health benefits. The qualitative characteristics of olive oil can be adversely affected by various biotic and abiotic factors. Particularly, microbial pathogens, such as mold fungi, can cause the deterioration of the oil and, thus, be a serious risk to consumer health. In this study, the effectiveness of DNA-based methods, i.e., endpoint PCR, Real-Time PCR (RT-PCR), and loop-mediated isothermal amplification (LAMP), all based on the ITS2-28S region, were used to evaluate the fungal contamination of samples of extra virgin olive oil. All the DNA techniques were able to detect, albeit at different levels, fungal infections affecting some of the basic quality parameters of the olive oils analyzed. However, compared to endpoint PCR and/or RT-PCR, the LAMP assay greatly simplified and accelerated the identification of pathogenic mold in the oil samples. This may encourage the olive oil industry to adopt this method in order to offer the consumer an oil with specific health parameters and therefore guarantee the safety and quality of this precious food product.

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Editorial: Current advancements in real-time plant pathogen diagnostics: from lab assays to in-field detection

Cited by 3 publications

References 10 publications

Magnetic Nanozyme-Enhanced Rapid ImmunoFlow-Through Assay for the Femtomolar Detection of Sugarcane Yellow Leaf Virus

Magnetic Nanozyme-Enhanced Rapid ImmunoFlow-Through Assay for the Femtomolar Detection of Sugarcane Yellow Leaf Virus

Does This Look Infected? Hidden Host Plant Infection by the Pathogen Botrytis cinerea Alters Interactions between Plants, Aphids and Their Natural Enemies in the Field

Assessing Olive Oil Quality Using Different DNA-Based Methods

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