EDL/MAE regulates EGF-mediated induction by antagonizing Ets transcription factor Pointed

Yamada, Teppei; Okabe, Masataka; Hiromi, Yasushi

doi:10.1242/dev.00624

Cited by 33 publications

(46 citation statements)

References 64 publications

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“…This pattern is then modulated in time by a negative-feedback that depends on the Br-mediated increase of tkv expression and Dpp signaling. The feedforward part of the model (outlined in blue) is supported by the previously published gain-and loss-of-function experiments with Pointed and EGFR signaling (Morimoto et al, 1996;Deng and Bownes, 1997;Yamada et al, 2003), and by our analysis of Br expression in ras -mosaics (Fig. 6).…”

Section: Discussionsupporting

confidence: 78%

Drosophilaeggshell is patterned by sequential action of feedforward and feedback loops

et al. 2008

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During Drosophila oogenesis, patterning activities of the EGFR and Dpp pathways specify several subpopulations of the follicle cells that give rise to dorsal eggshell structures. The roof of dorsal eggshell appendages is formed by the follicle cells that express Broad (Br), a zinc-finger transcription factor regulated by both pathways. EGFR induces Br in the dorsal follicle cells. This inductive signal is overridden in the dorsal midline cells, which are exposed to high levels of EGFR activation, and in the anterior cells, by Dpp signaling. We show that the resulting changes in the Br pattern affect the expression of Dpp receptor thickveins (tkv), which is essential for Dpp signaling. By controlling tkv, Br controls Dpp signaling in late stages of oogenesis and, as a result, regulates its own repression in a negative-feedback loop. We synthesize these observations into a model, whereby the dynamics of Br expression are driven by the sequential action of feedforward and feedback loops. The feedforward loop controls the spatial pattern of Br expression, while the feedback loop modulates this pattern in time. This mechanism demonstrates how complex patterns of gene expression can emerge from simple inputs, through the interaction of regulatory network motifs.

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Section: Discussionsupporting

confidence: 78%

Drosophilaeggshell is patterned by sequential action of feedforward and feedback loops

et al. 2008

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“…The echinoid-YFP trap line ed[CPTI000616] was acquired from the DGRC in Kyoto, Japan. The CY2-GAL4, UAS-Mae/CyO line was a gift from J. Duffy (WPI, Worcester, MA, USA); the effect of this genotype on eggshell patterning has been described previously (Yamada et al, 2003).…”

Section: Fly Stocksmentioning

confidence: 99%

Diversity of epithelial morphogenesis during eggshell formation in drosophilids

et al. 2015

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The eggshells of drosophilid species provide a powerful model for studying the origins of morphological diversity. The dorsal appendages, or respiratory filaments, of these eggshells display a remarkable interspecies variation in number and shape, and the epithelial patterning underlying the formation of these structures is an area of active research. To extend the analysis of dorsal appendage formation to include morphogenesis, we developed an improved 3D image reconstruction approach. This approach revealed considerable interspecies variation in the cell shape changes and neighbor exchanges underlying appendage formation. Specifically, although the appendage floor in Drosophila melanogaster is formed through spatially ordered neighbor exchanges, the same structure in Scaptodrosophila pattersoni is formed through extreme changes in cell shape, whereas Drosophila funebris appears to display a combination of both cellular mechanisms. Furthermore, localization patterns of Par3/Bazooka suggest a self-organized, cell polaritybased origin for the variability of appendage number in S. pattersoni. Our results suggest that species deploy different combinations of apically and basally driven mechanisms to convert a two-dimensional primordium into a three-dimensional structure, and provide new directions for exploring the molecular origins of interspecies morphological variation.

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“…a network in which an input activates both a target gene and its repressor (Kaplan et al, 2008;Lembong et al, 2009;Yakoby et al, 2008b). In this case, the input is provided by the single-peaked pattern of EGFR activation by GRK, the target gene is br, and its repression is mediated by PNT, an ETS-domain transcription factor (Boisclair Lachance et al, 2009;Lembong et al, 2009;Morimoto et al, 1996;Yamada et al, 2003). A repressive role for PNT is supported by the fact that eggshells derived from egg chambers with clones of pnt…”

Section: Research Articlementioning

confidence: 99%

Feedback control of the EGFR signaling gradient: superposition of domain-splitting events inDrosophilaoogenesis

et al. 2009

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The morphogenesis of structures with repeated functional units, such as body segments and appendages, depends on multi-domain patterns of cell signaling and gene expression. We demonstrate that during Drosophila oogenesis, the two-domain expression pattern of Broad, a transcription factor essential for the formation of the two respiratory eggshell appendages, is established by a single gradient of EGFR activation that induces both Broad and Pointed, which mediates repression of Broad. Two negativefeedback loops provided by the intracellular inhibitors of EGFR signaling, Kekkon-1 and Sprouty, control the number and position of Broad-expressing cells and in this way influence eggshell morphology. Later in oogenesis, the gradient of EGFR activation is split into two smaller domains in a process that depends on Argos, a secreted antagonist of EGFR signaling. In contrast to the previously proposed model of eggshell patterning, we show that the two-domain pattern of EGFR signaling is not essential for specifying the number of appendages. Thus, the processes that define the two-domain patterns of Broad and EGFR activation are distinct; their actions are separated in time and have different effects on eggshell morphology.

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EDL/MAE regulates EGF-mediated induction by antagonizing Ets transcription factor Pointed

Cited by 33 publications

References 64 publications

Drosophilaeggshell is patterned by sequential action of feedforward and feedback loops

Drosophilaeggshell is patterned by sequential action of feedforward and feedback loops

Diversity of epithelial morphogenesis during eggshell formation in drosophilids

Feedback control of the EGFR signaling gradient: superposition of domain-splitting events inDrosophilaoogenesis

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