eDNA‐based monitoring: Advancement in management and conservation of critically endangered killifish species

Abstract

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Effects of preservation strategies on environmental DNA detection and quantification using ddPCR

“…Species‐specific primer/probe assays compatible for ddPCR were developed following the methods outlined in Brys et al., 2020 and Mauvisseau et al., 2020, to quantify species‐specific eDNA concentrations of N. melanostomus, L. lota, and R. rutilus retrieved following the five different preservation treatments (see details of the assays development, including Limits of Detection and Limits of Quantification in Appendix S1 and Table S2). ddPCR analyses were conducted as in Mauvisseau et al., 2019 and Brys et al., 2020.…”

“…Species-specific primer/probe assays compatible for ddPCR were developed following the methods outlined in Brys et al, 2020 andMauvisseau et al, 2020, to S2). ddPCR analyses were conducted as in Mauvisseau et al, 2019 andBrys et al, 2020.…”

“…As a result, studies have been conducted, investigating various methodological elements of eDNA measurement procedures, allowing to increase the reliability and robustness of these approaches. Collection methods including filter type, pore size, extraction protocols or sample storage strategies and their impacts on eDNA recovery, and ultimately detection sensitivity, have already been extensively explored (Curtis et al., 2020; Djurhuus et al., 2017; Hinlo et al., 2017; Mauvisseau et al., 2020; Spens et al., 2017; Wegleitner et al., 2015; Yamanaka et al., 2016). Currently, on‐site filtration followed by rapid DNA extraction is generally recommended to reduce DNA degradation and obtain optimal DNA concentrations of specific target organisms or taxonomic groups (Hinlo et al., 2017; Yamanaka et al., 2016).…”

The value of quantitative environmental DNA analyses for the management of invasive and endangered native fish

“…Environmental DNA has proven valuable for monitoring single species at low densities (Ficetola et al., 2008; Ikeda et al., 2016; Sigsgaard et al., 2015); with applications that include improving species distribution estimates of invasive and endangered species (Bylemans et al., 2016; Doi, Katano, et al., 2017; Gold et al., 2020; Mauvisseau et al., 2020; Smart et al., 2015), evaluating eradication efforts (Davison et al., 2017; Furlan et al., 2019; Robinson et al., 2019), monitoring reintroductions and post‐release survival of species (Hempel et al., 2020; Rojahn et al., 2018), and determining the timing and location of reproductive activity (Bylemans et al., 2017; Erickson et al., 2016). Environmental DNA can also be used to obtain information on community composition through eDNA metabarcoding.…”

A review of the conservation status of seasonal Nothobranchius fishes (Teleostei: Cyprinodontiformes), a genus with a high level of threat, inhabiting ephemeral wetland habitats in Africa

“…It offers substantial potential for a method associated with highly repeatable and reliable results, as a rapid way to involve citizens in monitoring the presence of species through simple water sampling. Targeted environmental DNA surveys can be used for the assessment and conservation of threatened species (Thomsen & Willerslev, 2015; Mauvisseau et al, 2020).…”