EDTA‐Ammonium Carbonate Soil Test for Zinc

Abstract

A soil test was developed for the diagnosis of Zn‐deficient soils. The test consists of shaking 10 g soil with 20 ml of extractant (pH 8.6) containing 0.01M EDTA (ethylenediaminetetraacetic acid) and 1M (NH4)2CO3. After 30‐min shaking period the suspension is filtered, and Zn is determined directly in the filtrate by means of atomic absorption spectrophotometry. The new soil test was evaluated on 42 Colorado neutral and high lime soils whose response to Zn was determined in the greenhouse with corn (Zea mays) as the test crop. A critical level of 1.4 ppm of extractable Zn separated the Zn‐deficient from the nondeficient soils. The new Zn soil test compares favorably with the dithizone method and is an improvement over the 0.1N HCl method. Unlike strong‐acid extractants, the new procedure suppresses the dissolution of carbonates and oxides, and thereby avoids extraction of occluded Zn.

Distribution of Zinc, Manganese, Copper, Cobalt, and Nickel in Andosols Profiles

“…Soil organic carbon content was measured by Higashi (1986) (modified Tyurin method: Tyurin 1931). Soil sample solution for the determination of the total contents of Zn, Mn, Cu, Co, and Ni was prepared by the procedure of Trierweiler and Lindsay (1969). Four extraction methods [1 M ammonium acetate (NH4Ac) pH 4.5, 0.1 M HC1, 0.01 M ethylenediaminetetraacetic acid (EDTA) pH 6.5, and 0.005 M diethylenetriaminepentaacetic acid (DTPA) pH 7.3] were used for the determination of extractable Zn, Mn, Cu, Co, and Ni.…”

“…HC1 (0.1 M) extraction method: The contents of 0.1 M HC1 extractable heavy metals were determined according to the procedure of Trierweiler and Lindsay (1969).…”

Zinc-enriched fertilisers as a potential public health intervention in Africa

“…However, Zn deficiency in crops is widespread globally, in particular, due to low phytoavailability of Zn in soils. Such soils are commonly defined as having a Zn concentration extractable by ethylene diamine tetra-acetic acid (EDTA) or diethyl triamine penta-acetic acid (DTPA) less than 1.5 and 1.0 mg kg −1 DW of soil, respectively (Trierweiler and Lindsay 1969;Lindsay and Norvell 1978). Low phytoavailability of Zn can result from low soil Zn concentrations or the influence of soil characteristics that limit Zn solubility such as high pH values or large concentrations of available phosphate or CaCO 3 (Brümmer et al 1983;Graham et al 1992;White and Zasoski 1999;Cakmak 2002Cakmak , 2004Alloway 2008;Lu et al 2011).…”

Genotypic difference in cadmium effect on agronomic traits and grain zinc and iron concentration in winter wheat

“…Soil used in the experiment was collected from 0 -30 cm depth at Brestovac site located approximately 20 km from Osijek (45˚ 19' 47'' N. 17˚ 35' 44''). The experimental soil had 5.00 pH H2O , 3.83 pH KCl (ISO 10390;1994), 2.71 % humus (ISO 14235;1998) (Trierweiler and Lindsay, 1969).…”