EDTA-assisted heavy-metal uptake by poplar and sunflower grown at a long-term sewage-sludge farm

Liphadzi, M.S.; Kirkham, M.B.; Mankin, Kyle R.; Paulsen, Gary M.

doi:10.1023/a:1026294830323

Cited by 103 publications

(37 citation statements)

References 18 publications

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“…A statistically significant difference between species was evidenced in the phytoextraction of heavy metals: canola was the most effective in the uptake of Cu, Cd, Pb and Zn. Similar result were reported by Epstein et al (1999), Kirkham (2000), Liphadzi et al (2003), Thayalakumaran et al (2003), Turan and Angın (2004).…”

Section: Resultssupporting

confidence: 89%

Phytoremediation based on canola (Brassica napus L.) and Indian mustard (Brassica juncea L.) planted on spiked soil by aliquot amount of Cd, Cu, Pb, and Zn

Turan¹,

Esringü²

2007

PLANT SOIL ENVIRON

125

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The use of plants to remove heavy metals from soil (phytoremediation) is expanding due to its cost-effectiveness as compared to conventional methods and it has revealed a great potential. Since contaminants such as Pb or Cd have a limited bioavailability in the soil, methods to facilitate their transport to the shoots and roots of plants are required for successful phytoremediation. The objective of this study was to investigate the effects of addition of different rates (0, 3, 6 and 12 mmol/kg) of ethylene diaminetetraacetate (EDTA) on heavy metal availability in soils contaminated with 50 mg/kg Cd (CdCl 2 ), 50 mg/kg Cu (CuSO 4 ), 50 mg/kg Pb [Pb(NO 3 ) 2 ] and 50 mg/kg Zn (ZnSO 4 ), and on the capacity of canola (Brassica napus L.) and Indian mustard (Brassica juncea L.) plants to uptake Cu, Cd, Pb and Zn in a growth chamber. Results indicated that EDTA application increased heavy metal availability and uptake by plants. Significant differences were obtained in both species and plant parts. As for plant species tested, canola was more effective in the uptake of Cu, Cd, Pb and Zn. Root heavy metal uptake of both species was higher than shoot heavy metal uptake.

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Section: Resultssupporting

confidence: 89%

Phytoremediation based on canola (Brassica napus L.) and Indian mustard (Brassica juncea L.) planted on spiked soil by aliquot amount of Cd, Cu, Pb, and Zn

Turan¹,

Esringü²

2007

PLANT SOIL ENVIRON

125

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“…Chemically enhanced phytoextraction has been proposed as an effective approach for removing heavy metals from soils using plants (Huang et al, 1997;Blaylock et al, 1997;Liphadzi et al, 2003). Several chelating agents, such as citric acid, EDTA, CDTA, DTPA, EGTA, EDDHA, EDDS, HEDTA and NTA have been tested for their ability to mobilize and increase the accumulation of heavy metals, particularly Pb.…”

Section: Discussionmentioning

confidence: 99%

Enhanced phytoextraction of Cu, Pb, Zn and Cd with EDTA and EDDS

2005

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Chemically enhanced phytoextraction has been proposed as an effective approach to removing heavy metals from contaminated soil through the use of high biomass plants.Using pot experiments, the effects of the application of EDTA, EDDS and citric acid on the uptake of Cu, Pb, Zn and Cd by corn (Zea mays L. cv. Nongda 108) and bean (Phaseolus vulgaris L. white bean) plants were studied. The results showed that EDDS was more effective than EDTA at increasing the concentration of Cu in corn and beans.The application of 5 mmol kg -1 soil EDDS application to soil significantly increased concentrations of Cu in shoots , with maximum levels of 2060 and 5130 mg kg -1 DW in corn and beans, respectively, which were 45-fold and 135-fold higher than that in the corresponding control plants to which chelate had not been applied. Concentrations of Zn in shoots were also higher in the plants treated with EDDS than in those treated with EDTA. For Pb and Cd, EDDS was less effective than EDTA. The maximum Cu This is the Pre-Published Version.2 phytoextraction was found with the EDDS treatment. The application of EDTA and EDDS also significantly increased the shoot-to-root ratios of the concentrations of Cu, Pb, Zn and Cd in both plant species. The results of metal extraction with chelates showed that EDDS was more efficient at solubilizing Cu and Zn than EDTA, and that EDTA was better at solubilizing Pb and Cd than EDDS.

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“…With regard to metal phytoremediation, KOHLER et al (2004) characterized six metallothionein genes from a P. trichocharpa x P. deltoides hybrid, which conferred Cd tolerance when heterologously expressed in a Cd-hypersensitive yeast mutant. However, P. deltoides x P. nigra seedlings showed no special ability to hyperaccumulate Cd, Ni, Pb, Cu, Fe, Mn or Zn when grown on sewage sludge-treated soil, even when the soil was treated with EDTA (LIPHADZI et al, 2003).…”

Section: Forest Trees As Hyperaccumulatorsmentioning

confidence: 94%

Engineering Forest Trees with Heavy Metal Resistance Genes

Merkle

2006

Silvae Genetica

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Pollution of soil and water with heavy metals such as mercury, cadmium and arsenic, is a worldwide problem. Phytoremediation, the use of plants to remove, sequester or detoxify pollutants, including heavy metals, offers an environmentally-friendly alternative to engineering-based methods for remediation. Forest trees have multiple features that make them particularly useful for removal of toxic heavy metals, especially if they can be engineered with genes allowing them to handle high levels of these elements. Although still in its infancy, research with transgenic trees carrying genes allowing them to detoxify or sequester some heavy metals has already made promising progress. Most of the work to date has been performed using poplar species and hybrids, although other woody species could be equally as useful. Trees have been engineered with genes for the handling of mercury, cadmium, copper and arsenic following two main approaches, phytoextraction and phytotransformation /phytovolatilizaton. In vitro studies have shown the transgenic trees to have enhanced abilities to tolerate and/or accumulate these metals, and preliminary results from field tests indicate that the trees are functioning. New combinations of genes involved in metal transport or conversion may further enhance the heavy metal remediation capabilities of the transgenic trees. Given the environmentally friendly application, forest trees engineered for phytoremediation may be some of the first transgenic forest trees approved for operational deployment.

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EDTA-assisted heavy-metal uptake by poplar and sunflower grown at a long-term sewage-sludge farm

Cited by 103 publications

References 18 publications

Phytoremediation based on canola (Brassica napus L.) and Indian mustard (Brassica juncea L.) planted on spiked soil by aliquot amount of Cd, Cu, Pb, and Zn

Phytoremediation based on canola (Brassica napus L.) and Indian mustard (Brassica juncea L.) planted on spiked soil by aliquot amount of Cd, Cu, Pb, and Zn

Enhanced phytoextraction of Cu, Pb, Zn and Cd with EDTA and EDDS

Engineering Forest Trees with Heavy Metal Resistance Genes

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