Daughters and mothers have the great amount of reciprocal disclosure in adolescent-parent family relationships. Mothers are first teachers for their daughtersregarding puberty. It's important to encouragemothers-daughters effective communication.Aim of the study:Was to assess mothers and their adolescent daughters' communication regarding pubertal changes at Tanta City.Materials and Methods: A descriptive cross-sectional study design was used in this study. Setting: This study was conducted in the governmental preparatory schools, Tanta city.Subjects: The total number of the study subjects was 400 mothers and their adolescent daughters.Data collection Tools: A structured questionnaire schedulewas prepared by the researchers.Itcomposed of two parts. Part I: for girls, that covered girls' source of knowledge about pubertal changes and menstrual hygiene, and their reaction to pubertal changes. Part II:mothers' communication and preparation of daughters for pubertal changes. Results: Slightly more than one third (35.0%) of mothers communicate with their daughters to prepare them for puberty.The majority of the studied mothers (88%) imposed restriction to their daughters after puberty.There were a statistically significant positive correlation between mothers' communication about puberty with daughters and their education, occupation, and number of children (P< 0.05). Conclusionand recommendations: About two thirds of mothers did not communicate with their daughters to prepare them for puberty. The majority of the studied mothers imposed restriction to their daughters after puberty. Friends were the main source of knowledge about puberty for more than half of the studied daughters. Educational programs aboutpubertal changesare recommended to be performed to adolescent daughters and their mothers.