Background: Knowledge translation experts advocate for employing knowledge brokers (KBs) to promote the uptake of research evidence in health care settings. However, little is known about factors influencing the utilization of KBs, thereby limiting their employment within healthcare organizations. This research aimed to identify factors likely to hinder or promote the optimal use of KBs within rehabilitation settings in Canada.Methods: Qualitative study using semi-structured telephone interviews with individuals performing KB activities in rehabilitation settings across Canada. The interview topic guide was informed by the Consolidated Framework for Implementation Research (CFIR) and consisted of 20 questions covering five domains (characteristics of individuals, inner setting, process, outer settings, and innovation characteristics). All interviews lasted 60 to 90 minutes, were digitally recorded, and transcribed verbatim. We conducted qualitative descriptive analysis combining deductive coding guided by the CFIR. Two independent analysts coded and rated all interviews, then met to review, deliberate and modify the codes as appropriate. A matrix was created by listing the salient codes for each CFIR construct to identify factors (facilitators and barriers) at the individual, organisational, and process level most likely to impact the KB’s success/failure.Results: Twenty-three participants, from five Canadian provinces were interviewed. At the individual level, the majority of participants reported having strong communication skills, being confident about performing KBs activities, and possessing solid clinical experience and prior research skills. At the organizational level, most respondents indicated constantly networking and engaging with clinical teams and different stakeholders, and having an acceptable level of guidance from their managers. Very few participants felt that they received sufficient organizational support (i.e., clerical support and IT support). At the process level, all participants indicated needing evaluation tools to better gauge their performance, and the majority mentioned that they would benefit from having additional training tailored to their roles as KBs.Conclusions: Individual, organisational and process level factors likely to hinder or promote the optimal use of KBs within Canadian rehabilitation settings include skillsets and networking abilities; culture, resources, and leadership support; and the need for specific training for KBs and for evaluation tools to monitor their performance.