Eefect of Subtilin Ointment on Morphological Indicators of Wound Healing in Rabbits

Abstract

The paper studies the effect of applying probiotics based Subtilin ointment on morphological indicators of the experimental wound healing in rabbits. The study showed that 3 days after traumatic skin damage and applying Subtilin ointment tissue necrosis was found on the tissue specimen. Marked cell infiltration in interfascicular spaces and swollen collagen fibers were found deep in the skin. The wound surface was covered with a thick fibrin and tissue crust. On day 7 active regeneration of the epithelium proliferating under the crust was observed. Native granulation tissue formed under the newly formed epithelium. On day 14 epithelization and granulation processes resulted in forming fibrous connective tissue. The fibrous collagen elements of the new tissue thickened, formed bundles and prevailed over the cellular elements, fibroblasts. On day 21 the epidermis formed layers (stratum basale, stratum spinosum and stratum corneum). The connective tissue had a developed capillary network, tissues under the regenerating area had regular structure but contained regenerating hair follicles. On day 28 epithelization was completed and two types of regenerated connective tissue formed. The first type was fibrous connective tissue made up of thin bundles of collagen fibers where fibroblastic cells and thin-wall capillaries were found. The second type of regenerated connective tissue was similar to regular connective tissue in structure. By that time only appendages of the skin such as hair sheaths sebaceous and sudoriferous glands could not fully regenerate. Thus, application of Subtilin ointment for treating experimental skin wounds in rabbits relieves signs of inflammation, accelerates complete epithelization of the wound on day 28, and forms native granulation tissue under the epithelium that gradually matures to regenerated connective tissue. The structure of the regenerated connective tissue is similar to that of regular connective tissue.