Efecto de la obesidad inducida por dieta en el tejido testicular y parámetros de estrés oxidativo en el suero

Erdemır, Fikret; Atılgan, Doğan; Markoç, Fatma; Boztepe, Ozgur; Suha-Parlaktas, B.; Sahin, Şevki

doi:10.1016/j.acuro.2011.06.019

Cited by 53 publications

(22 citation statements)

References 27 publications

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“…Eventually, oxidative damage causes an increase in tissue levels of malondialdehyde (MDA) and protein carbonyl (PC), which are the end products of lipid peroxidation and protein oxidation, respectively, [16]. Many studies have shown that obesity induced OS and this phenomenon led to tissue damage [17, 18]. …”

Section: Introductionmentioning

confidence: 99%

Weight Loss and Melatonin Reduce Obesity-Induced Oxidative Damage in Rat Testis

Atılgan

Parlaktaş

Uluocak

et al. 2013

Advances in Urology

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Aim. We aimed to evaluate the antioxidant effects of weight loss and melatonin on the obesity-induced oxidative damage in rat testes. Materials and Methods. 28 male Wistar albino rats were randomly divided into 4 groups, each consisting of 7 rats: control group (Group 1), obesity group (Group 2), obesity + MLT group (Group 3), and weight loss group (Group 4). Rats were weighed at the beginning and at the end of the study. Bilateral orchiectomy was performed and 5 cc blood samples were obtained from all of the rats. Superoxide dismutase (SOD), malondialdehyde (MDA), and protein carbonyl (PC) levels were analysed in the testicular tissues and serum. Spermatogenesis was evaluated with the Johnsen scoring system. Results. The testicular tissue and serum levels of MDA, PC, and SOD activity were increased in the obesity group in comparison to the sham operated group (P < 0.05). Weight loss and melatonin treatment ameliorated MDA, PC, and SOD levels in testicular tissue and serum significantly (P < 0.05). There was no significant difference between groups in terms of mean Johnsen score (P = 0.727). Conclusion. Experimentally created obesity caused oxidative stress and both melatonin and weight loss reduced oxidative stress parameters in rat testes.

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Section: Introductionmentioning

confidence: 99%

Weight Loss and Melatonin Reduce Obesity-Induced Oxidative Damage in Rat Testis

Atılgan

Parlaktaş

Uluocak

et al. 2013

Advances in Urology

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“…A Peak Scale Loupe 7x (GWJ Company, Hacienda Heights, CA, USA) micrometer was used as a scale calibration standard to calculate the diameters, expressed in micrometers (μm). Spermatogenesis was quantified with Johnsen’s scoring system (Johnsen, 1970), as modified by Erdemir et al (2012). In the extratubular compartment, edema, hemorrhagic extravasation, venular and/or lymphatic vessels dilation, and Leydig cells changes were evaluated according to a previously described method (Minutoli et al, 2005, 2015a), scoring each parameter with the following scale: 0, absent; 1, mild; 2, moderate; 3, severe.…”

Section: Methodsmentioning

confidence: 99%

Polydeoxyribonucleotide, an Adenosine-A2A Receptor Agonist, Preserves Blood Testis Barrier from Cadmium-Induced Injury

et al. 2017

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Cadmium (Cd) impairs blood-testis barrier (BTB). Polydeoxyribonucleotide (PDRN), an adenosine A2A agonist, has positive effects on male reproductive system. We investigated the effects of PDRN on the morphological and functional changes induced by Cd in mice testes. Adult Swiss mice were divided into four groups: controls administered with 0.9% NaCl (1 ml/kg, i.p., daily) or with PDRN (8 mg/kg, i.p. daily), animals challenged with Cd chloride (CdCl2; 2 mg/kg, i.p, daily) and animals challenged with CdCl2 (2 mg/kg, i.p., daily) and treated with PDRN (8 mg/kg, i.p., daily). Experiments lasted 14 days. Testes were processed for biochemical, structural, and ultrastructural evaluation and hormones were assayed in serum. CdCl2 increased pERK 1/2 expression and Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH) levels; it decreased testosterone (TE) and inhibin-B levels and induced structural damages in extratubular compartment and in seminiferous epithelium, with ultrastructural features of BTB disruption. Many TUNEL-positive germ cells were present. CdCl2 increased tubular TGF-β3 immunoreactivity and reduced claudin-11, occludin, and N-cadherin immunoreactivity. PDRN administration reduced pERK 1/2 expression, FSH, and LH levels; it increased TE and inhibin-B levels, ameliorated germinal epithelium changes and protected BTB ultrastructure. Few TUNEL-positive germ cells were present and the extratubular compartment was preserved. Furthermore, PDRN decreased TGF-β3 immunoreactivity and enhanced claudin-11, occludin, and N-cadherin immunoreactivity. We demonstrate a protective effect of PDRN on Cd-induced damages of BTB and suggest that PDRN may play an important role against Cd, particularly against its harmful effects on gametogenesis.

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“…Mild diet restriction and supplementation with antioxidant rich foods or pure compounds were reported to protect obese subjects from oxidative stress and improve fertility (Roushandeh et al, 2015). Diet restricting an obese mutant of three age groups had metabolically beneficial effect, but (Erdemir et al, 2012;Jensen et al, 2004;Kort et al, 2006;MacDonald et al, 2010;Michalakis, Mintziori, Kaprara, Tarlatzis, & Goulis, 2013;Zhao, Zhai, Liu, Wu, & Xu, 2014). Further it was observed that mild diet restriction improved the duration of reproductively active periods in various animal models (Roushandeh et al, 2015;Suleiman, Ali, Zaki, EL-MALIK, & Nasr, 1996).…”

Section: Discussionmentioning

confidence: 99%

“…Diet restricting an obese mutant of three age groups had metabolically beneficial effect, but fat, circulatory lipids and oxidative stress can also affect the reproductive behaviour of mammals. Obese subjects are known to have lower testosterone, and it is strongly associated with body fat percentage, lipid levels and oxidative stress (Erdemir et al, 2012;Jensen et al, 2004;Kort et al, 2006;MacDonald et al, 2010;Michalakis, Mintziori, Kaprara, Tarlatzis, & Goulis, 2013;Zhao, Zhai, Liu, Wu, & Xu, 2014). Thus, it can be speculated that these factors may directly or via lowered testosterone levels act on neuroendocrine axis to reduce libido.…”

Section: Discussionmentioning

confidence: 99%

Pre-pubertal diet restriction reduces reactive oxygen species and restores fertility in male WNIN/Obese rat

et al. 2017

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Obesity is a multifactorial disorder associated with increased body adiposity, chronic oxidative stress which contributes to impaired fertility in males. Diet restriction and anti-oxidant supplementations are known to protect obese subjects from oxidative stress and improves fertility. However, the role of oxidative stress and the age of intervention in restoring male fertility are poorly understood. This study was aimed to assess the effect of diet restriction on fertility with respect to the age of intervention, body composition and oxidative stress using WNIN/Ob obese mutant rat strain. Unlike lean and carrier phenotypes, obese rats are hyperphagic, hyperlipaemic and infertile. Male obese rats aged for 35, 60 and 90 days were fed either ad libitum or diet restricted for 6 weeks. Upon diet restriction mean body weight, total body fat percentage, circulatory lipids and oxidative stress markers were significantly reduced and it follows the order as 35 < 60 < 90 days. Diet-restricted males of the three age groups were allowed to mate with female carrier rats, and fertility was restored only in 35-day group. Diet restriction in male obese WNIN/Ob rats lowered the rate of body weight gain, with reduced oxidative stress overall and fertility restoration in groups intervened at pre-pubertal stages.

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Efecto de la obesidad inducida por dieta en el tejido testicular y parámetros de estrés oxidativo en el suero

Cited by 53 publications

References 27 publications

Weight Loss and Melatonin Reduce Obesity-Induced Oxidative Damage in Rat Testis

Weight Loss and Melatonin Reduce Obesity-Induced Oxidative Damage in Rat Testis

Polydeoxyribonucleotide, an Adenosine-A2A Receptor Agonist, Preserves Blood Testis Barrier from Cadmium-Induced Injury

Pre-pubertal diet restriction reduces reactive oxygen species and restores fertility in male WNIN/Obese rat

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