Efecto De La Zeolita Natural Clinoptilita Sobre El Crecimiento De Dunaliella Salina (Teodoresco, 1905) Cultivada en Un Fotobiorreactor De Múltiples Cámaras Oscilantes

Abstract

We studied the growth and production of pigment of microalgae Dunaliella salina cultivated in<br />photobioreactor multicamera oscillating outside laboratory conditions to evaluate the effect of different<br />concentrations of zeolite (ZC). Growth was evaluated by cell count and pigment content was performed<br />by spectrophotometric techniques. The results indicate that the concentration of 50 mgL-1 of ZC produced<br />a better stimulus on the growth of the microalga reaching maximum cell density (MDC) of 5.51 ±0.45 x 106<br />celmL-1, growth rate (μ) 0.37 ±0.03 divday-1 and duplication time (Td) of 1.87 ±0.02 days. Likewise,<br />produced a greater increase in the total chlorophyll and carotenoids in the logarithmic phase of values<br />15.554 ±0.77 and 0.50 ±0.01 mgmL-1, respectively. Chloroplastic pigments concentration per volume of<br />culture has a significant correlation with maximum cell density of D. salina treatments based on zeolite at<br />all stages of growth with rMDC, chla.tot= 0.89 y una rMDC, carot.tot=0.926 at a level of significance (p <0.01). The<br />results demonstrated the feasibility of using this product as a suitable substrate for the growth of the<br />microalga, being an innovative alternative and less costly to obtain significant metabolites.