Efecto de polifenoles de la dieta mediterránea sobre la proliferación y mediadores de la invasividad “in vitro” de la línea de cáncer vesical murino MB-49

Mediero, J.M. García; Ferruelo, Antonio; Borda, Á. Páez; Galán, M. Lujan; Angulo, Javier C.; Robles, Vicente Chiva; Sánchez, A. Berenguer

doi:10.1016/s0210-4806(05)73335-0

Cited by 9 publications

(2 citation statements)

References 25 publications

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“…However, those conventional therapies can not last long because of systemic toxicity and local irritation [2]. Since resveratrol is non-toxic [5] and exerts inhibitory effects on TCC cells [9]–[12], it would be an alternative candidate for the management of TCCs if its anti-TCC capacity can be further proved under the experimental conditions closer to the clinical statuses. To mimic intravesical drug instillation, the cultured EJ TCC cells were treated with 100 µM, 150 µM or 200 µM resveratrol transiently instead of constantly.…”

Section: Discussionmentioning

confidence: 99%

“…A body of evidence shows that resveratrol is able to inhibit the growth of many cancers such as leukemia, breast cancer and primary brain tumors [6]–[8]. In the case of bladder cancers, resveratrol effectively decreases cell viability and induces apoptosis of human and murine bladder cancer cells [9]–[12]. Nevertheless, the practical value of resveratrol in anti-TCC therapy has not been addressed by the use of more clinically relevant experimental model(s) and in the way of local drug administration.…”

Section: Introductionmentioning

confidence: 99%

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Short-Term Resveratrol Exposure Causes In Vitro and In Vivo Growth Inhibition and Apoptosis of Bladder Cancer Cells

Liu

et al. 2014

PLoS ONE

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Conventional adjuvant chemotherapies for bladder transitional cell carcinomas (TCCs) may cause strong systemic toxicity and local irritation. Non-toxic resveratrol inhibits TCC cell growth but its feasibility in clinical management of TCCs remains obscure. This study aimed to evaluate the safety and anti-TCC efficacy of resveratrol, using the experimental models closer to the clinical treatment condition. Human TCC EJ cells were exposed to 100 µM, 150 µM and 200 µM resveratrol respectively for 1 hour and 2 hours to mimic intravesical drug instillation and the cell responses were analyzed by multiple experimental approaches. An orthotopic TCC nude mouse model was established by injecting EJ cells into the sub-urothelial layer and used for short-term intravesical resveratrol instillation. The safety of resveratrol instillation was evaluated and compared with that of MCC. The results revealed that 2 h 150 µM or 200 µM resveratrol treatment leaded to remarkable S phase arrest and apoptosis at 72 h time-point, accompanied with attenuated phosphorylation, nuclear translocation and transcription of STAT3, down-regulation of STAT3 downstream genes (survivin, cyclinD1, c-Myc and VEGF) and nuclear translocations of Sirt1 and p53. The importance of STAT3 signaling in cell growth was confirmed by treating EJ cells with JAK2 inhibitor tyrphostin AG490. The efficacy and safety of resveratrol instillation were proved by the findings from nude mouse orthotopic xenograft models, because this treatment caused growth suppression, distinctive apoptosis and STAT3 inactivation of the transplanted tumors without affecting normal urothelium. Our results thus suggest for the first time the practical values of resveratrol as a safe and effective agent in the post-operative treatment of TCCs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Short-Term Resveratrol Exposure Causes In Vitro and In Vivo Growth Inhibition and Apoptosis of Bladder Cancer Cells

Liu

et al. 2014

PLoS ONE

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Resveratrol: The Biochemistry Behind Its Anticancer Effects

Kundu

Surh

2009

Plant Phenolics and Human Health

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Effect of Phenolic Compounds Against Aβ Aggregation and Aβ-Induced Toxicity in Transgenic C. elegans

Jagota

Rajadas

2011

Neurochem Res

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Substantial evidence suggests that the aggregation of amyloid-β (Aβ) peptide into fibrillar structures that is rich in β-sheets is implicated as the cause of Alzheimer's disease. Therefore, an attractive therapeutic strategy is to prevent or alter Aβ aggregation. Phenolic compounds are natural substances that are composed of one or more aromatic phenolic rings and present in wine, tea, fruits, vegetables and a wide variety of plants. In this work, we investigated the effects of ferulic acid, morin, quercetin and gossypol against Aβ aggregation. From the ThT and turbidity assays, it is observed that in addition to the fibril aggregate, another type of aggregate is formed in the presence of morin, quercetin, and gossypol. On the other hand, ferulic acid did not prevent fibril formation, but it did appear to reduce the average length of fibrils compared to Aβ alone. To study the protective effects of phenolic compounds on Aβ-induced toxicity, we utilized the nematode Caenorhabditis elegans (C. elegans) as an in vivo model organism, human Aβ is expressed intracellularly in the body wall muscle. We found that exposure of Caenorhabditis elegans to ferulic acid give more protection against Aβ toxicity than morin, quercetin and gossypol.

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Efecto de polifenoles de la dieta mediterránea sobre la proliferación y mediadores de la invasividad “in vitro” de la línea de cáncer vesical murino MB-49

Abstract: The polyphenols present in our usual diet exert an effect on the proliferation and mediators of bladder tumor invasiveness in MB-49 cells.

Cited by 9 publications

References 25 publications

Short-Term Resveratrol Exposure Causes In Vitro and In Vivo Growth Inhibition and Apoptosis of Bladder Cancer Cells

Short-Term Resveratrol Exposure Causes In Vitro and In Vivo Growth Inhibition and Apoptosis of Bladder Cancer Cells

Resveratrol: The Biochemistry Behind Its Anticancer Effects

Effect of Phenolic Compounds Against Aβ Aggregation and Aβ-Induced Toxicity in Transgenic C. elegans

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