Efecto del Propóleos Chileno sobre el Metabolismo de Glucosa en Ratones Diabéticos

Pacheco, Alejandro; Daleprane, Julio Beltrame; Freitas, Vanessa Sabino de; Ferderbar, Simone; Hirabara, Sandro Massao; Cuevas, Alejandro; Saavedra, Nicolás; Curi, Rui; Abdalla, Dulcinéia Saes Parra; Salazar, Luis A.

doi:10.4067/s0717-95022011000300015

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…Similarly, the Chilean propolis has showed different biological properties, including antiangiogenic [6–8], antiatherosclerotic [7, 9], antifungal [10], antidiabetic [11], antimicrobial [12–16], hepatoprotective [17], antiproliferative [18], and antioxidant [18] activities, due to its high content of polyphenols, mainly pinocembrin [13].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Alternative Methods to Assess the Biological Properties of Propolis on Metabolic Activity and Biofilm Formation in Streptococcus mutans

Veloz

Alvear

Salazar

2019

Evidence-Based Complementary and Alternative Medicine

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Several biological activities have been reported for the Chilean propolis, among their antimicrobial and antibiofilm properties, due to its high polyphenol content. In this study, we evaluate alternative methods to assess the effect of Chilean propolis on biofilm formation and metabolic activity of Streptococcus mutans (S. mutans), a major cariogenic agent in oral cavity. Biofilm formation was studied by using crystal violet and by confocal microscopy. The metabolic activity of biofilm was evaluated by MTT and by flow cytometry analysis. The results show that propolis reduces biofilm formation and biofilm metabolic activity in S. mutans. When the variability of the methods to measure biofilm formation was compared, the coefficient of variation (CV) fluctuated between 12.8 and 23.1% when using crystal violet methodology. On the other hand, the CV ranged between 2.2 and 3.3% with confocal microscopy analysis. The CV for biofilm’s metabolic activity measured by MTT methodology ranged between 5.0 and 11.6%, in comparison with 1.9 to 3.2% when flow cytometry analysis was used. Besides, it is possible to conclude that the methods based on colored compounds presented lower precision to study the effect of propolis on biofilm properties. Therefore, we recommend the use of flow cytometry and confocal microscopy in S. mutans biofilm analysis.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Alternative Methods to Assess the Biological Properties of Propolis on Metabolic Activity and Biofilm Formation in Streptococcus mutans

Veloz

Alvear

Salazar

2019

Evidence-Based Complementary and Alternative Medicine

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Antibiofilm Activity of Chilean Propolis onStreptococcus mutansIs Influenced by the Year of Collection

Veloz

Saavedra

Lillo

et al. 2015

BioMed Research International

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The chemical composition of propolis varies according to factors that could have an influence on its biological properties. Polyphenols from propolis have demonstrated an inhibitory effect on Streptococcus mutans growth. However, it is not known if different years of propolis collection may affect its activity. We aimed to elucidate if the year of collection of propolis influences its activity on Streptococcus mutans. Polyphenol-rich extracts were prepared from propolis collected in three different years, characterized by LC-MS and quantified the content of total polyphenols and flavonoids groups. Finally, was evaluated the antibacterial effect on Streptococcus mutans and the biofilm formation. Qualitative differences were observed in total polyphenols, flavones, and flavonols and the chemical composition between the extracts, affecting the strength of inhibition of biofilm formation but not the antimicrobial assays. In conclusion, chemical composition of propolis depends on the year of collection and influences the strength of the inhibition of biofilm formation.

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Polyphenols from Chilean Propolis and Pinocembrin Reduce MMP-9 Gene Expression and Activity in Activated Macrophages

Saavedra

Cuevas

Cavalcante

et al. 2016

BioMed Research International

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Polyphenols from diverse sources have shown anti-inflammatory activity. In the context of atherosclerosis, macrophages play important roles including matrix metalloproteinases synthesis involved in degradation of matrix extracellular components affecting the atherosclerotic plaque stability. We prepared a propolis extract and pinocembrin in ethanol solution. Propolis extract was chemically characterized using LC-MS. The effect of treatments on gene expression and proteolytic activity was measured in vitro using murine macrophages activated with LPS. Cellular toxicity associated with both treatments and the vehicle was determined using MTT and apoptosis/necrosis detection assays. MMP-9 gene expression and proteolytic activity were measured using qPCR and zymography, respectively. Thirty-two compounds were identified in the propolis extract, including pinocembrin among its major components. Treatment with either ethanolic extract of propolis or pinocembrin inhibits MMP-9 gene expression in a dose-dependent manner. Similarly, an inhibitory effect was observed in proteolytic activity. However, the effect showed by ethanolic extract of propolis was higher than the effect of pinocembrin, suggesting that MMP-9 inhibition results from a joint contribution between the components of the extract. These data suggest a potential role of polyphenols from Chilean propolis in the control of extracellular matrix degradation in atherosclerotic plaques.

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Efecto del Propóleos Chileno sobre el Metabolismo de Glucosa en Ratones Diabéticos

Cited by 3 publications

References 15 publications

Evaluation of Alternative Methods to Assess the Biological Properties of Propolis on Metabolic Activity and Biofilm Formation in Streptococcus mutans

Evaluation of Alternative Methods to Assess the Biological Properties of Propolis on Metabolic Activity and Biofilm Formation in Streptococcus mutans

Antibiofilm Activity of Chilean Propolis onStreptococcus mutansIs Influenced by the Year of Collection

Polyphenols from Chilean Propolis and Pinocembrin Reduce MMP-9 Gene Expression and Activity in Activated Macrophages

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