Efecto del suplemento de astaxantina sobre la calidad seminal en Moenkhausia sanctaefilomenae (Teleostei: Characidae)

Domínguez-Castanedo, Omar; Toledano-Olivares, Ángel; Ávalos-Rodrı́guez, Alejandro

doi:10.3856/vol43-issue1-fulltext-18

Cited by 3 publications

(3 citation statements)

References 37 publications

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“…This is higher than values obtained with another species from the Moenkhausia genus. The sperm motility rate for M. sanctaefilomenae was just over 50% (Domínguez-Castanedo et al, 2015). The use of a hormonal inducer may have elevated the motility rate of M. oligolepis sperm, as observed by Ferreira et al (2001) with the jundiá, Rhamdia quelen.…”

Section: Discussionmentioning

confidence: 77%

“…The sperm concentration found in the studied species is similar to other species from the same genus. M. sanctaefilomenae was reported to have an average sperm concentration of 6.8 × 10 8 sperm per ml semen (Domínguez-Castanedo et al, 2015). However, variations in seminal characteristics are common in fish, both between different species and individuals of the same species (Silva et al, 2009;Murgas et al, 2011).…”

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confidence: 99%

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Seminal characterization of the Amazonian fire-eye tetra Moenkhausia oligolepis (Günther, 1864)

Rodrigues

Santos

Cordeiro

et al. 2020

Zygote

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Summary The seminal characteristics of Moenkhausia oligolepis are described. Three males were induced with a single dose of carp pituitary. Semen was collected 6 h after induction, and diluted in dibasic sodium phosphate extender solution. For motility analysis, 1 µl of diluted semen was added to 10 µl of distilled water to achieve gamete activation. The average duration of total motility was 76.67 s; while the average sperm motility rate at intervals of 15 s was 95.3, 85.3, 59.6, 31.7, 13.0, 4.6 and 1.2%. To determine sperm concentration in samples, 0.5 μl of semen was diluted with 500 μl of glutaraldehyde. An aliquot of 10 μl of this dilution was utilized for cell counting. An average count of 4.97 × 109 ± 3.46 sperm/ml was obtained. Morphological analyses were performed using eosin–nigrosine dye; 20.33% of the sperm were observed to be dead. Live sperm, comprising the other 79.67%, had an average length of approximately 30 µm, with a head diameter of 4.488 ± 0.7 µm; and a flagella plus mid-piece length of 26.071 ± 12.4 µm. Of those sperm, 69% had a normal morphology, while 31% had primary and secondary abnormalities. The observed abnormality rate did not have a detrimental effect on artificial fertilization potential for the species. The description of the seminal characteristics of a species is one of the most important sets of information required for artificial reproduction of fish in captivity. It also contributes significantly to the total biological knowledge of the studied species.

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Section: Discussionmentioning

confidence: 77%

mentioning

confidence: 99%

Seminal characterization of the Amazonian fire-eye tetra Moenkhausia oligolepis (Günther, 1864)

Rodrigues

Santos

Cordeiro

et al. 2020

Zygote

View full text Add to dashboard Cite

show abstract

“…To date, a very few studies have tried to improve the antioxidant capacity of semen, applying supplementation through diet (Domínguez‐Castanedo et al . 2015), while in most cases, antioxidants have been supplemented directly in the extenders (Cabrita et al . 2011; Lahnsteiner et al .…”

Section: Supplementation Of Antioxidants In Cryopreservation Mediummentioning

confidence: 99%

Oxidative stress and use of antioxidants in fish semen cryopreservation

Sandoval‐Vargas

Jiménez

González

et al. 2020

Reviews in Aquaculture

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Reactive oxygen species (ROS) have been proposed as one of the main causes of the impairment of fish spermatozoa integrity and functionality during cryopreservation. The high content of unsaturated fatty acids in sperm cells and the low antioxidant capacity of diluted semen are key factors in making sperm cells susceptible to ROS attacks. For this reason, some recent studies have determined the antioxidant status of the seminal plasma and spermatozoa of fish species. Additionally, some studies have evaluated the effects of antioxidants on post-thaw sperm quality. Although ROS are certainly involved in sperm damage, other factors, such as ice crystal formation, seem to play a crucial role in cryodamage. This challenge has not yet been resolved because both the endogenous antioxidant capacity of the semen and its response to different supplementation practices seem to present specific inter-and intraspecies characteristics and effects. This review summarises knowledge on antioxidant defence and oxidative stress in fish semen, as well as antioxidant supplementation in cryopreservation media, in order to establish perspectives for future studies.

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Effect of Oxidative Stress on Sperm Cells

Izquierdo¹,

Reyes²,

Muñiz³

et al. 2020

Glutathione System and Oxidative Stress in Health and Disease

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Free radicals are unstable molecules that have an unpaired electron in their last orbital, which makes them highly unstable agents. In medicine, it has been discovered that they play an important role in cell signaling and without them some cells such as leukocytes or sperm could not perform their biological functions. To protect itself from these oxidizing agents, the cell has a defense system based on antioxidants; however, when this balance is lost and oxidizing agents exceed the cellular antioxidant capacity, the cell enters oxidative stress, which affects cellular components such as proteins, nucleic acids, lipids, amino acids, and carbohydrates, among others. In the case of spermatozoa, due to their high metabolic rate, they produce large quantities of oxygen reactive species (ROS), decreasing sperm motility, alterations in cytoplasmic components, modifications in genetic material, or sperm death. In this chapter, a review is made of a brief history of how the toxicity of oxygen and free radicals was discovered, the oxidative stress in cells, and the effect of oxidative stress in the cytoplasmic sperm membrane, in the spermatic mitochondria, in the spermatic acrosome, in the sperm DNA, and in the fertility of the female and the male.

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Efecto del suplemento de astaxantina sobre la calidad seminal en Moenkhausia sanctaefilomenae (Teleostei: Characidae)

Cited by 3 publications

References 37 publications

Seminal characterization of the Amazonian fire-eye tetra Moenkhausia oligolepis (Günther, 1864)

Seminal characterization of the Amazonian fire-eye tetra Moenkhausia oligolepis (Günther, 1864)

Oxidative stress and use of antioxidants in fish semen cryopreservation

Effect of Oxidative Stress on Sperm Cells

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