Efeito citotóxico da crotoxina em células de melanoma murino e fibroblastos.

Paioli, Fernanda Somma

doi:10.11606/d.87.2011.tde-17032011-105046

Cited by 3 publications

(6 citation statements)

References 77 publications

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“…During evolution, snakes have specialized in affecting the vital functions of their prey by releasing a large number of toxins (enzymes, proteins and peptides) through venom that destabilize the physiological levels of hormones, alter the activity of enzymes, receptors or ion channels, and promote cardiovascular and nervous system imbalance in their prey. The use of snake toxins as pharmacological tools and prototypes for drug development is increasing (Paioli, 2011).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic activity and abdominal writhes promoted by snake venom from Philodryas nattereri Steindachner, 1870

Nery

Aquino

Ribeiro

et al. 2014

Fundam. Toxicol. Sci.

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Philodryas nattereri is distributed in arid and semiarid regions of South America and is most common in northeastern Brazil. The aims of the work were to investigate the effects of the venom from P. nattereri in cultured of MDCK and RAW cells and abdominal writhes in mice. Based on oxidative metabolism, it was possible to observe that the venom was capable of significantly reducing cell viability only at higher concentrations of venom at 50 and 100 μg/mL for MDCK cells, while in 200 μg/mL to RAW cells, with an IC 50 of 169.5 μg/mL. Regarding writhing in mice promoted by the poison and acetic acid, it held a greater number of writhes when compared to promoted by saline. The venom of P. nattereri has a cytotoxic effect in MDCK and RAW cells and abdominal writhes, which appears to be similar to those caused by acetic acid.

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Section: Introductionmentioning

confidence: 99%

Cytotoxic activity and abdominal writhes promoted by snake venom from Philodryas nattereri Steindachner, 1870

Nery

Aquino

Ribeiro

et al. 2014

Fundam. Toxicol. Sci.

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“…Ao fracionar o veneno total de uma serpente, pode-se encontrar enzimas como serinoproteinases, metaloproteinases, acetilcolinesterases (AchEs), L-aminoácido oxidases (LAAOs), fosfolipases A 2 (PLA 2 ) e B , nucleotidases (5'-nucleotidases, ATPases, fosfodiesterases e DNases) e hialuronidases (Markland, 1998;Kang et al;2011) e entre as toxinas com atividade não enzimáticas estão as toxinas tipo three-finger, as disintegrinas e as lectinas tipo C (Sajevic et al;2011).…”

Section: Venenos Ofídicosunclassified

Componentes derivados de venenos de serpentes com ação antitumoral em células de melanoma Murino

Queiroz¹

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Despite the constant advances in the treatment of cancer, this disease remains one of the main causes of mortality worldwide. So, the development of new treatment modalities is imperative. Snake venom causes a variety of biological effects because they constitute a complex mixture of substances as disintegrins, proteases (serine and metalo), phospholipases A2, L-amino acid oxidases and others. The goal of the present work is to evaluate a anti-tumor activity of some snake venoms fractions. There are several studies of components derived from snake venoms with this kind of activity. After fractionation of snake venoms of the families Viperidae and Elapidae, the fractions were assayed towards murine melanoma cell line B16-F10 and fibroblasts L929. The results showed that the fractions of venom of the snake Notechis ater niger had higher specificity and potential antitumor activity on B16-F10 cell line than the other studied venoms.Since the components of this venom are not explored yet coupled with the potential activity showed in this work, we decided to choose this venom to develop further studies. The cytotoxic fractions were evaluated to identify and characterize the components that showed antitumoral activity. Western blot assays and zymography suggests that these proteins do not belong to the class of metallo and serine proteinases.

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“…A serpente Crotalus durissus terrificus conhecida popularmente como cascavel é encontrada no Brasil desde o estado do Rio Grande do Sul até o de Minas Gerais. Os efeitos de seu veneno se manifestam amplamente no organismo humano como, por exemplo, o sistema nervoso autônomo, rins, músculos esqueléticos, sangue e diversos órgãos (Paioli, 2011).…”

Section: Veneno Da Crotalus Durissus Terrificusunclassified

“…Na década de 1970 teve inicio o uso de crotoxina como um agente antitumoral, através de um fármaco chamado VRCTC-310 composto por crotoxina e cardiotoxina provindos do veneno da serpente Naja naja atra, havendo resposta positiva na diminuição dos tumores (Costa et al, 1998;Paioli, 2011). Em relação a PLA 2 (Fosfolipase A2), apesar do seu mecanismo de ação ainda não ser completamente esclarecido, sua atividade catalítica é importante para o efeito antiproliferativo (Donato et al, 1996).…”

Section: Efeitos Da Crotoxina Em Célulasunclassified

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Isolamento e caracterização da crotoxina símile do veneno de Crotalus vegrandis com atividade antitumoral

Nishimura¹

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Several studies have shown that some snake venom components have efficient antitumor activity in vitro and in vivo. The venom of the rattlesnake Crotalus vegrandis has neurotoxic, myotoxic and hemorrhagic activities. The crotoxina-like is an important component of this venom and is formed by an acidic protein (crotapotin) combined to a basic subunit (PLA 2). As there are few studies on Crotalus vegrandis venom, the isolation and characterization of its components and an investigation of their potential and efficacy as a therapeutic agent against tumor cells would be of great value. In this study, we carried out the isolation and characterization of the crotoxin-like of Crotalus vegrandis and assayed its cytotoxicicity on L929 and B16F10 cells. We carried out the cultivation of these cells in 96 well plates, to then be placed in direct contact with the different purified fractions Crotalus vegrandis venom for a total time of 48 hours. For comparison, we carried out the same test with the purified fractions of Crotalus durissus terrificus venom. The MTT assay was used to assess the cell viability after treatment with fractions of Crotalus vegrandis and Crotalus durissus terrificus venoms. The results showed a high cytotoxic activity for both venoms in B16F10 tumor cells and little toxicity to normal L929 cells. Also in comparative tests with both venoms, we observed that for a given concentration, the fractions of the venom of Crotalus vegrandis had higher cytotoxic effect than the Crotalus durissus terrificus venom, with greater specificity for B16F10 cells.

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Efeito citotóxico da crotoxina em células de melanoma murino e fibroblastos.

Cited by 3 publications

References 77 publications

Cytotoxic activity and abdominal writhes promoted by snake venom from <i>Philodryas nattereri</i> Steindachner, 1870

Cytotoxic activity and abdominal writhes promoted by snake venom from <i>Philodryas nattereri</i> Steindachner, 1870

Componentes derivados de venenos de serpentes com ação antitumoral em células de melanoma Murino

Isolamento e caracterização da crotoxina símile do veneno de <i>Crotalus vegrandis</i> com atividade antitumoral

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