Efeito combinado da aplicação de células tronco e laser de baixa potência sobre a cicatrização do tendão calcanear de ratos após transecção parcial

Abstract

LETÍCIA DRUDI LUCKEEfeito combinado da aplicação de células tronco e laser de baixa potência sobre a cicatrização do tendão calcanear de ratos após transecção parcial Combined effect of the low-level laser and stem cells application on the healing of the calcaneal tendon of rats after partial transection

Evaluation of Biological Membrane and Stem Cells as Potential Repairs of Tenorraphy in Wistar Rats

“…Animal (LMPA-HVET-UENF), kept under controlled temperature (22ºC ±2ºC) and relative humidity (55% ±5%) and a 12/12-hour photoperiod, receiving balanced food and water ad libitum. In addition to the 31 adult animals, 3 young rats, approximately 1 month old, were euthanized by infusion of anesthetics (2% xylazine and 10% ketamine), according to one of the indications for small laboratory rodents (LUCKE, 2018). An incision of the cutaneous and subcutaneous tissue was then made in the inguinal region, gaining access to the adipose tissue.…”

“…In the search to improve and accelerate regenerative processes, research has been carried out in different tissues such as skin (KOTTON et al, 2001), bone (KOSACHENCO et al, 1998), nerves (STAINKI et al, 1995) and muscles and tendons (REDDY et al, 1998;BOTTAGISIO and LOVATI, 2017). In particular, the study of tendon repair has been growing, since tissue recovery after injury is still a major challenge in clinical practice (LUCKE, 2018).…”

“…Soon after the injury, the inflammatory sequence lasts for three to seven days, characterized by changes in endothelial cells resulting from blood vessel injury, and activation of platelets that undergo degranulation and secrete multiple mediators, forming clots. At the end of this phase, the beginning of angiogenesis, stimulation of tenocyte proliferation, the beginning of collagen III production and also the expression of cytokines responsible for activating anti-inflammatory pathways such as the deactivation of macrophages and natural killer cells (LUCKE, 2018;CLUTTERBUCK et al, 2010;MORITA et al, 2017).…”

“…From the 14th day to the 21st day, the third and final phase of the healing process begins, remodeling, which can last up to 1 year after the injury (ABATE et al, 2009). At this stage, there is a marked change in the proportion of collagen type, where collagen III is degraded while a large amount of collagen I is degraded (LUCKE, 2018;RAISER, 2001).…”

“…Stem cells constitute a population of undifferentiated cells with unique characteristics of self-renewal and plasticity. These cells are present in all tissues throughout life and are involved in the processes of embryogenesis and regeneration (LUCKE, 2018). Fundamentally, there are two types of stem cells: the embryonic stem cell (ESC), which comes from the inner mass of the blastocyst, and the adult stem cell, which is divided into a mesenchymal stem cell (MSC) and a hematopoietic stem cell (HSC).…”