Efeito da L-arginina na neoproliferação intimal e no remodelamento arterial após lesão por balão, em ilíacas de coelhos hipercolesterolêmicos

Abstract

objective: It has been implied that neointimal proliferation and remodeling are the major causes of restenosis. The objective of this study is to assess the effect of orally administered L-arginine on these two factors in hypercholesterolemic rabbits that had suffered an injury to their iliac arteries caused by a catheter balloon. Methods:The study included nineteen rabbits that were divided in two groups: control (CG) and arginine (AG). There were 19 arteries studied from the control group and 17 in the arginine group. The animals were placed on a 2% hypercholesterolemic diet for 15 days and then submitted to a balloon angioplasty in order to produce a lesion in their iliac arteries. Next, the AG animals were given a 1g/kg/day oral dose of a L-arginine solution. The animals were sacrificed 15 days after the angioplasty procedure and histological artery sections were prepared, stained and fixed. The ratio between the neointimal area (in mm 2 ) and the media layer (in mm 2 ) was used to represent lesion development. In order to determine remodeling, the ratio between the total area of the medial portion of the vessel (greater balloon contact) and the total area of the reference segment of the vessel (less balloon contact) was used.results: Mean neointimal thickness (NI/M) was 0.8151±0.2201 in CG and 0.3296±0.1133 in AG. Remodeling patterns for the two groups studied were similar. conclusion: In the experimental model used, L-arginine was able to reduce intimal tissue thickness in hypercholesterolemic rabbits but did not act on artery remodeling.

Aterosclerose experimental em coelhos

“…L-arginine (a NOS substrate) depletion in animal models of atherosclerosis and hypercholesterolemia induces platelet aggregation, cell proliferation, and vascular monocyte accumulation, whereas endothelial-dependent vasoreactivity is improved in hypercholesterolemic rabbits treated with L-arginine, thus attenuating the mechanisms of vascular lesion 26 and inhibiting neointimal proliferation 27 , which can be assessed by means of several endothelial cell markers proposed (Table 1).…”

Allium sativum Compared to Cilostazol as an Inhibitor of Myointimal Hyperplasia

“…The balloon diameter used was safe and effective, according to Ferrer et al [25] , Gellman et al [13] , and other researchers [14] .…”

Experimental Atherosclerosis in Rabbits

“…The development of interventions aimed at inhibiting atherosclerosis from cholesterol, and vascular dysfunction have received much attention because of this important association. L-arginine (a NOS substrate) depletion in animal models of atherosclerosis and hypercholesterolemia induces platelet aggregation, cell proliferation, and vascular monocyte accumulation, whereas endothelial-dependent vasoreactivity is improved in hypercholesterolemic rabbits treated with L-arginine, thus attenuating the mechanisms of vascular lesion 26 and inhibiting neointimal proliferation 27 , which can be assessed by means of several endothelial cell markers proposed (Table 1).…”