Efeito da L-Glutamina sobre o perfil leucocitário e a função fagocítica de macrófagos de ratos estressados

Abstract

ObjetivoVerificar o efeito da administração intraperitoneal de glutamina, prévia a uma situação de estresse, sobre o número de leucócitos do sangue e sobre a taxa de fagocitose de macrófagos alveolares de ratos. MétodosRatos machos Wistar aos 90 dias de idade foram divididos em controle (C, n=15), estresse (C+E, n=16), glutamina (GLN, n=15) e glutamina e estresse (GLN+E, n=15) e mantidos em condições padronizadas. Os grupos GLN e GLN+E receberam, via intraperitoneal (v ip), L-glutamina (0,2g/kg de peso corporal) durante 10 dias. Seus pares receberam, v ip, L-glicina (0,4g/kg do peso corporal). Os animais C+E e GLN+E foram submetidos ao estresse agudo de contenção (40min, após 24h da última intervenção com o aminoácido). Leucócitos totais foram contados em hemocitômetro e os diferenciais por esfregaço sanguíneo. Para avaliação da taxa de fagocitose, os macrófagos alveolares (1x10 6 em meio de cultura RPMI) e fungos S. cerevisiae (1x10 7 ) foram incubados em estufa (37ºC, 5%CO 2 ) durante 1 hora. Foram utilizados os testes estatísticos análise de variância (ANOVA) e o teste de Tukey, valores expressos em média do percentual e desvio-padrão.

Heparin fails to inhibit the leukocyte recruitment for an extended time following inflammatory stimulus

“…The proportion of lymphocytes in the whole blood for the saline group was higher than neutrophils. This result is according to other experiments conducted in Wistar rats and mice, for the mentioned populations and for the other analyzed populations: monocytes and eosinophils (Crockett et al, 2004;Kreimer et al, 2005;Lagasse & Weissman, 1996;Leandro et al, 2006). All the groups that received thioglycollate stimulus presented a higher proportion of neutrophils in the whole blood when compared with the saline group, which correlates with the inflammatory state of the rat (Lagasse & Weissman, 1996).…”

Hematological and immunological effects of stress of air traffic controllers in northeastern Brazil

“…(32) There are some studies (5) in animals and humans that demonstrate that stress reduces the total leukocyte count, thus corroborating our results.…”

“…Many studies demonstrate that stress and emotional reactions affect the immune system. ( 4 , 5 ) The most common way to identify possible alterations in the immunological system is by complete blood counts, including the hematopoietic system, leukocyte profile or biochemical markers of stress. ( 4 , 5 ) Some works ( 5 , 6 ) have demonstrated that even before the feedback system, characterized by a constant turnover of cells, which need constant replication to maintain the leukocyte, platelet and erythrocyte populations, stress can affect the number and quality of these cells.…”

“…( 4 , 5 ) The most common way to identify possible alterations in the immunological system is by complete blood counts, including the hematopoietic system, leukocyte profile or biochemical markers of stress. ( 4 , 5 ) Some works ( 5 , 6 ) have demonstrated that even before the feedback system, characterized by a constant turnover of cells, which need constant replication to maintain the leukocyte, platelet and erythrocyte populations, stress can affect the number and quality of these cells. ( 7 ) However, when comparing blood counts, certain variables that affect the blood system, such as the time of collection due to alterations in the circadian rhythm, and the presence of viruses and infections such as in tonsillitis, must be taken into account.…”

Air traffic control activity increases attention capacity in air traffic controllers

“…Some studies have demonstrated that stress affects the immune 2 , 3 and nervous systems with regard to the cognitive functions 4 including that of attention. 5 Since 1980, behavior tests have been developed to evaluate attention and investigate the nervous circuits related to these processes.…”