Efeito da suplementação dietética de arginina na cicatrização das anastomoses colônicas em ratos

Corrêa-Neto, Milton Pereira; Campos, Antônio Carlos; Branco, Alessandra Borges; Matias, Jorge Eduardo Fouto

doi:10.1590/s0102-67202009000100003

Cited by 3 publications

(3 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many studies have shown clear benefit of the use of immune- modulating enteral formulas by various mechanisms, because they enhancing the immune status in its various aspects 11 . However, the use of individual components present in these formulas did not bring such positive evidences and literature is divergent as regards to its results 4 , 5 . The omega-3 polyunsaturated fatty acids have been shown to play an important role in the mechanisms involving cellular signaling pathways that culminate with an anti-inflammatory effect.…”

Section: Discussionmentioning

confidence: 96%

“…However, due to the complex mechanism of interaction between absorption processes, membranes attachment and intracellular signaling, it is known that formulas consisting of amino acids, essential fatty acids, trace elements, vitamins, nucleotides and nucleosides, were effective when using together, while the use of specific nutrients alone was associated with less clear benefits and with divergent results 5 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Omega-3 Fatty Acid in the Healing Process of Colonic Anastomosis in Rats

Castilho

Campos

Mello

2015

ABCD, arq. bras. cir. dig.

View full text Add to dashboard Cite

Background : The use of long-chain polyunsaturated fatty acids has been studied in the context of healing and tissue regeneration mainly due to its anti-inflammatory, immunoregulatory and antioncogenic properties. Previous studies have demonstrated beneficial effects with the use of enteral immunonutrition containing various farmaconutrients such as L-arginine, omega-3, trace elements, but the individual action of each component in the healing of colonic anastomosis remains unclear. Aim : To evaluate the influence of preoperative supplementation with omega-3 fatty acids on the healing of colonic anastomoses of well-nourished rats. Methods : Forty Wistar adult male rats, weighing 234.4±22.3 g were used. The animals were divided into two groups: the control group received for seven days olive oil rich in omega-9 oil through an orogastric tube, while the study group received isocaloric and isovolumetric omega-3 emulsion at a dose of 100 mg/kg/day, also for seven days. Both groups were submitted to two colotomies followed by anastomosis, in the right and left colon, respectively. Parameters evaluated included changes in body weight, anastomotic complications and mortality, as well as maximum tensile strength by using a tensiometer and collagen densitometry at the anastomotic site. Results : There were no differences in body weight or mortality and morbidity between groups. The value of the maximum tensile strength of the control group was 1.9±0.3 N and the study group 1.7±0.2, p=0.357. There was, however, a larger amount of type I collagen deposition in the study group (p=0.0126). The collagen maturation índex was 1.74±0.71 in the control group and 1.67±0.5 in the study group; p=0,719). Conclusions : Preoperative supplementation of omega-3 fatty acid in rats is associated with increased collagen deposition of type I fibers in colonic anastomoses on the 5th postoperative day. No differences were observed in the tensile strength or collagen maturation index.

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Effect of Omega-3 Fatty Acid in the Healing Process of Colonic Anastomosis in Rats

Castilho

Campos

Mello

2015

ABCD, arq. bras. cir. dig.

View full text Add to dashboard Cite

show abstract

“…Considering these findings, the present study aimed to compare the healing processes in the abdominal wall of rats, according to biochemical and histopathological parameters, after treatment with arginine+PBS solution (arginine group) or PBS solution (control group), both injected intraperitoneally. Experimental studies in rats were performed to verify the effects of arginine on postoperative healing of intestines and skin, burns, incorporation of synthetic material in the abdominal wall, with different doses and routes of administration, such as oral, subcutaneous and intraperitoneal[16][17][18][19][20] .…”

mentioning

confidence: 99%

Efeitos da arginina na reparação cicatricial da parede abdominal em ratos Wistar.

Caron

Repka

Milgioransa

et al. 2019

Rev. Col. Bras. Cir.

View full text Add to dashboard Cite

RESUMO Objetivo: avaliar os efeitos da arginina na cicatrização da parede abdominal de ratos Wistar. Métodos: vinte ratos Wistar foram submetidos à laparotomia e separados em dois grupos (arginina e controle), que receberam tratamento diário por via intraperitoneal com arginina (300mg/kg/dia) e solução tampão fosfato em dose equivalente ao peso, respectivamente, durante cinco dias. No sétimo dia pós-operatório, coletaram-se amostras de sangue e da cicatriz da parede abdominal de ambos os grupos. Avaliaram-se o nível sérico de nitratos e nitritos, a evolução cicatricial pelas dosagens de hidroxiprolina tecidual, formação de tecido de granulação, determinação da porcentagem de colágeno maduro e imaturo, densidade de miofibroblastos e angiogênese. Empregaram-se os testes de ANOVA e t de Student com p=0,05 para as comparações entre os grupos. Resultados: não ocorreram diferenças significantes entre os grupos estudados para dosagens de nitratos e nitritos (p=0,9903), hidroxiprolina tecidual (p=0,1315) e densidade de miofibroblastos (p=0,0511). O grupo arginina apresentou maior densidade microvascular (p=0,0008), maior porcentagem de colágeno tipo I (p=0,0064) e melhora na formação do tecido de granulação, com melhores índices de proliferação angiofibroblástica (p=0,0007) e re-epitelização das bordas (p=0,0074). Conclusão: na avaliação cicatricial da parede abdominal de ratos Wistar sob tratamento com arginina, não houve alteração do nível sérico de nitratos e nitritos, da deposição de colágeno total e da densidade de miofibroblastos. Verificaram-se aumento da maturação de colágeno do tipo I, da densidade microvascular e melhora na formação do tecido de granulação cicatricial pelas melhores re-epitelização de bordas e proliferação angiofibroblástica.

show abstract

Hydrogel containing (1 → 6)-β-D-glucan (lasiodiplodan) effectively promotes dermal wound healing

Nissola

Marchioro

Mello

et al. 2021

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

Efeito da suplementação dietética de arginina na cicatrização das anastomoses colônicas em ratos

Cited by 3 publications

References 35 publications

Effect of Omega-3 Fatty Acid in the Healing Process of Colonic Anastomosis in Rats

Effect of Omega-3 Fatty Acid in the Healing Process of Colonic Anastomosis in Rats

Efeitos da arginina na reparação cicatricial da parede abdominal em ratos Wistar.

Hydrogel containing (1 → 6)-β-D-glucan (lasiodiplodan) effectively promotes dermal wound healing

Contact Info

Product

Resources

About