Efeito de citocininas na senescência e abscisão foliar durante o cultivo in vitro de Annona glabra L.

Oliveira, Lenaldo Muniz de; Paiva, Renato; Santana, José Raniere Ferreira de; Nogueira, Raírys Cravo; Soares, Fernanda Pereira; Silva, Luciano Coutinho

doi:10.1590/s0100-29452007000100008

Cited by 13 publications

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“…The presence of iron has been related to the degree of oxidation of explants maintained in in vitro conditions, and this element is an important constituent of the enzymes liberated as a result of cell damage, and which initiate a series of oxidative response processes. The observed increase in the iron content is interesting as woody species generally demonstrate high levels of tissue oxidation during in vitro establishment -which represents one of the limiting factors in using this technique with this group of plants, especially the Annonaceae (Santana et al, 2003;Oliveira et al, 2007 Ciênc. agrotec., Lavras, v. 34, n. 6, p. 1439Lavras, v. 34, n. 6, p. -1445Lavras, v. 34, n. 6, p. , nov./dez., 2010 Our results indicate the necessity of developing new formulations for the essential minerals used in in vitro culture media, taking into consideration the presence of growth regulators and the mineral composition of the cultivated tissues themselves.…”

Section: Resultsmentioning

confidence: 99%

“…Many attempts have been made to cultivate (Zobayed et al, 2002;Nagori & Purohit, 2004) wild Annonaceae species (Santana et al, 2003;Oliveira et al, 2007Oliveira et al, , 2008Santana et al, 2008a,b) through micropropagation, but low levels of explant multiplication, slow shoots growth rates, and elevated explant mortality during acclimatization have contributed to the limited commercial success of this technique.…”

Section: Introductionmentioning

confidence: 99%

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Effects of cytokinins on in vitro mineral accumulation and bud development in Annona glabra L.

et al. 2010

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Annona glabra is a tropical species that has significant agronomic potential in terms of furnishing fruits for in natura consumption and for the production of phyto-pharmaceuticals. In vitro cultivation has been considered the most promising form of propagation for this species, although large scale utilization of this technique is currently limited by high rates of leaf abscission, reduced rates of explant multiplication and slow bud growth. The present work evaluated the effects of different cytokinins on mineral accumulation in shoots of A. glabra cultivated in vitro, and their effects on growth and survival of these plants. Buds of A. glabra were cultivated in Wood Plant Medium (WPM) in the presence of 6-benzilaminopurine (BAP), thidiazuron (TDZ), kinetin (KIN), and zeatin (ZEA). KIN and BAP use resulted in the greatest growth, largest accumulation of dry mass and leaf area development, as well as the greatest survival rate during in vitro cultivation of this species. All cytokinins tested stimulated large accumulations of nitrogen and boron in shoots, but diminished levels of calcium as compared to controls.Index terms: Growth regulators, Annonaceae, mineral nutrition. RESUMOAnnona glabra é uma espécie frutífera tropical que apresenta elevado potencial agronômico pelo fornecimento de frutos para o consumo in natura e pela produção de fitofármacos. O cultivo in vitro tem sido preconizado como a forma mais adequada de propagação para essa espécie, embora sua utilização em larga escala ainda seja limitada pela elevada taxa de abscisão foliar, reduzida taxa de multiplicação dos explantes e crescimento lento das brotações. Objetivou-se, neste trabalho, avaliar o efeito de citocininas sobre o acúmulo de minerais nas brotações de A. glabra cultivadas in vitro e seus reflexos sobre o crescimento e sobrevivência das plantas nesse tipo de ambiente. Brotações de A. glabra foram cultivadas em meio Wood Plant Medium (WPM), na presença de 6-benzilaminopurina (BAP), thidiazuron (TDZ), cinetina (KIN) e zeatina (ZEA). A utilização de KIN e BAP induziu maior crescimento, maior acúmulo de massa seca, maior desenvolvimento da área foliar e maior taxa de sobrevivência das plantas durante o cultivo in vitro dessa espécie. Todas as fontes de citocininas testadas estimularam maiores acúmulos de nitrogênio e boro nas brotações e menores de cálcio.Termos para indexação: Reguladores de crescimento, Annonaceae, nutrição mineral.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of cytokinins on in vitro mineral accumulation and bud development in Annona glabra L.

et al. 2010

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“…Dentre os fatores que favorecem a morfogênese in vitro, destacam-se os reguladores de crescimento e outras substâncias químicas que induzem à citocinese, causando alterações na taxa metabólica, na atividade enzimática, na indução e na formação de órgãos, na quebra de dominância apical, na mobilização de nutrientes orgânicos e inorgânicos e no aumento da longevidade de tecidos e órgãos (Oliveira et al, 2007). Normalmente, essas substâncias que induzem a embriogênese são utilizadas em baixas concentrações, pois podem apresentar toxicidade quando em excesso.…”

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Efeito do Glifosato no cultivo in vitro de Cattleya nobilior Rchb. F

et al. 2018

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Effect of Glyphosate on in vitro culture of Cattleya nobilior Rchb. FAmong the techniques used by biotechnology for multiplication of orchid seedlings, the in vitro cultivation stands out due to the high production potential of new seedlings. The presence of some chemical substances used in the culture medium, depending on the concentration, may or may not favor the growth and development of the plant. Therefore, the objective of this work was to evaluate the effect of glyphosate on the in vitro cultivation of the orchid Cattleya nobilior Rchb. F. The experimental design was completely randomized, with six treatments and ten replications. The treatments consisted of six concentrations of glyphosate (0.0, 8.5, 17.0, 25.5, 34.0, and 42.5 mg L -1 a.i.), added to the modified MS culture medium with half the concentrations of macronutrients. Seedlings subjected to different glyphosate concentrations were obtained from seeds germinated in vitro. The length and dry biomass measurements of the shoot and root, number of leaves, leaf length, leaf width, number of shoots, chlorophyll a, b, and carotenoid contents were performed 200 days after transplanting of the seedlings. Among the phytometric components analyzed, only the length and dry biomass of root and the carotenoid content decreased with increasing doses of the glyphosate herbicide. Low concentrations of glyphosate added to the culture medium result in hormese effect, with increased growth of Cattleya nobilior Rchb. F. shoot.Keywords: hormone; micropropagation; Orchidaceae. RESUMO ABSTRACTEfeito do Glifosato no cultivo in vitro de Cattleya nobilior Rchb. F

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“…Annona glabra L. (Annonaceae) is a fruit species that has been extensively researched due to its favorable agronomic characteristics, serving as a grafting stock for several cultivated Annonaceae (Oliveira et al 2007) due to its phytochemical properties, with antibacterial, antifungal, insecticidal and cytotoxic effects (Padmaja et al 1995;Oliveira et al 2008). However, the commercial exploitation of this species as a rootstock for cultivated Annonaceae, and as a source of bioactive compounds or even for the recovery of degraded areas of vegetation has been limited by the difficulty of obtaining healthy specimens, especially in large quantities (Hoffmann et al 1996).…”

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confidence: 99%

“…Clonal propagation via in vitro culture has been a viable alternative for the multiplication of several species (e.g., Azevedo et al 2008;Castro et al 2008;Schiavinato et al 2008;Victorio et al 2008), including A. glabra (Oliveira et al 2007;Santana et al 2008a,b). However, obtaining an efficient protocol for in vitro multiplication of Annonaceae on a commercial scale is still a challenge.…”

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Influence of flask sealing and activated charcoal on the morphogenesis and leaf anatomy of Annona glabra (Annonaceae) cultured in vitro

Santana¹,

Paiva²,

Oliveira³

et al. 2011

Sitientibus sér. Ci. Biol.

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This study evaluated the effects of the type of tube closure and the use of activated charcoal in the morphogenesis and leaf anatomy of Annona glabra (Annonaceae). Nodal segments of A. glabra were inoculated in WPM medium supplemented with 30 g l-1 sucrose and five concentrations of activated charcoal (0, 1, 2, 3 and 4 g l-1). During in vitro culture, nodal segments were kept in test tubes closed in one of three ways: plastic cap sealed with PVC wrap, plastic cap without a PVC wrap and cotton plug. The results showed that the shoots obtained in tubes closed with a plastic cap without a PVC wrap or sealed with a cotton plug had increased growth compared to those obtained in tubes sealed with a plastic cap and a PVC wrap. The presence of activated charcoal caused a reduction of leaf abscission and influenced the anatomical development of the shoots in the tubes sealed with a cotton plug and sealed with a plastic cap without a PVC wrap. The highest means for leaf tissue thickness were obtained from the shoots kept in closed tubes with a plastic lid and a PVC wrap in the absence of activated charcoal.

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Efeito de citocininas na senescência e abscisão foliar durante o cultivo in vitro de Annona glabra L.

Cited by 13 publications

References 15 publications

Effects of cytokinins on in vitro mineral accumulation and bud development in Annona glabra L.

Effects of cytokinins on in vitro mineral accumulation and bud development in Annona glabra L.

Efeito do Glifosato no cultivo in vitro de Cattleya nobilior Rchb. F

Influence of flask sealing and activated charcoal on the morphogenesis and leaf anatomy of Annona glabra (Annonaceae) cultured in vitro

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