Efeito do miriadenolídeo isolado de Alomia myriadenia (Asteraceae) sobre o tumor de Erlich ascítico no camundongo

Junior, Duarte; Souza-Fagundes, Elaine M.; Cassali, Geovanni Dantas; Ribeiro, Eduardo Magalhães; Zani, Carlos L.; Melo, Marília Martins

doi:10.1590/s0102-09352006000500014

Cited by 9 publications

(4 citation statements)

References 20 publications

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“…As described in literature, it is known that only the presence of Ehrlich tumor is able to induce the appearance of renal toxicity. 16 It is noteworthy that both formulations containing CDDP provoked a decrease of the urea/creatinine index, achieving similar values to those found for healthy mice treated with saline in previous studies (118.0 ± 7.0). 8 For the TR7 treatment schedule, it was not possible to detect any difference of the urea/creatinine index between all treatments.…”

Section: Resultssupporting

confidence: 85%

Antitumor effectiveness and toxicity of cisplatin-loaded long-circulating and pH-sensitive liposomes against Ehrlich ascitic tumor

Maroni

Silveira

Leite

et al. 2012

Exp Biol Med (Maywood)

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Cisplatin (CDDP) is one of the most active cytotoxic agents commonly used in the treatment of peritoneal carcinomatosis. The disadvantages of its clinical use are systemic side-effects, such as nephrotoxicity and myelotoxicity. Long-circulating and pH-sensitive liposomes containing CDDP (SpHL-CDDP) were developed by our research group aiming to promote the release of CDDP near the tumor as well as decreasing toxicity. The aim of this study was to evaluate the antitumor efficacy and toxicity of SpHL-CDDP after intraperitoneal administration in initial or disseminated tumor-bearing mice, at a dose of 12 mg/kg. The survival was monitored and blood samples were collected for biochemical and hematological analysis. Kidneys, liver and spleen were removed for histopathological examination. Tumor cells were evaluated for cellular viability and cell cycle. The survival of animals treated with SpHL-CDDP was higher than those treated with free CDDP. The cell death caused by treatment with SpHL-CDDP occurred through induction of apoptosis, with a cell cycle arrest at the G0/G1 phase. The treatment of mice presenting initial cancer with both formulations provoked a suppression of granulocytes. Mice treated with free CDDP also showed a decrease in platelet count, which suggests a high myelotoxicity. In an advanced cancer model, SpHL-CDDP treatment allowed an improvement of the immune response. Mice affected by cancer at an early stage and treated with free CDDP or SpHL-CDDP showed a lower urea/creatinine index compared with the saline control group. These findings indicate that both treatments were able to reduce the renal damage caused by peritoneal carcinomatosis. Microscopic analysis of kidneys from mice treated with SpHL-CDDP showed a discrete morphological alteration, while tubular necrosis was observed for free CDDP-treated mice. Concerning hepatotoxicity, no alteration in clinical chemistry parameters was observed. These findings reveal that SpHL-CDDP can improve the antitumor efficacy and decrease renal and bone marrow toxicity.

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Section: Resultssupporting

confidence: 85%

Antitumor effectiveness and toxicity of cisplatin-loaded long-circulating and pH-sensitive liposomes against Ehrlich ascitic tumor

Maroni

Silveira

Leite

et al. 2012

Exp Biol Med (Maywood)

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“…Moreover, their use can cause stress in animals by changing their behavior (Galsworthy et al, 2005) due to the emitted electrical signals. Thus, the method which remains the most effective is the visual assessment (Souza et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

“…With this parameter, the differentiation between HG and IG is easy because when the infection process is installed, mice become debilitated, cachectic, with generalized edema, regulated by the concentration of TNF-alpha and other immunological markers (Truyens et al, 1999). This is a good clinical parameter to analyze, but in order not to make any mistakes in the evaluation, generating data with excellence, the animal must be immobilized and the measurement performed in the same anatomical site, by a single experimenter, eliminating variations due to the size of the hand and the way the mice are manipulated, avoiding too much stretching and compression of the animal's skin (Verçosa Júnior et al, 2006).…”

Section: Methodsmentioning

confidence: 99%

Parameters for evaluation of clinical trial in mice infected with Trypanosoma cruzi

Falkowski¹,

Sandri

Tiyo³

et al. 2012

Arq. Bras. Med. Vet. Zootec.

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The effectiveness of clinical parameters in the evaluation of Trypanosoma cruzi infection was analyzed in male Swiss mice at 8 weeks old Animals were divided into HG (healthy) and IG (1400 trypomastigotes, intraperitoneally, Y strain -Trypanosoma cruzi). Quantitative and qualitative parameters were evaluated in non-consecutive days in the period, from 7 th -11 th and 15 th -18 th days of infection. There were significant differences (P<0.05) between both groups in both periods regarding water consumption, abdominal circumference and weight. The second group presented differences in amount of excreta, body temperature, move-up and mortality. There was no difference (P>0.05) between the groups in food consumption, exploration of self-cleaning and skin staining. The fecal feature differed between the groups in the second period. The occurrence of isolation was not practical. Differences were observed in the hair between groups, although the parameter had been interfered by fights between animals. The consumption of water, feed, excreta production, characteristic of the faeces, body temperature, abdominal circumference, move up, weight and mortality parameters are easy to be measured and effective in clinical differentiation of healthy mice infected with T. cruzi, elected in protocols for clinical study with mice, which is the first work to gather information of qualitative and quantitative clinical parameters evaluated in these animals. Keywords: clinical analysis, Chagas disease, experimental infection, mice, Trypanosoma cruzi RESUMO Analisou-se a eficiência de parâmetros clínicos na avaliação da infecção pelo Trypanosoma cruzi em camundongos suíços, machos de 8 semanas. Os grupos foram divididos em GS (sadios) e GI (1400 tripomastigotas, intraperitoneal, cepa Y -Trypanosoma cruzi

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“…(HCM) e concentração de hemoglobina corpuscular media (CHCM), contagem total e diferencial de leucócitos e presença de linfócitos reativos (Verçosa Júnior et al, 2006). As amostras foram processadas pelo princípio de fotometria no equipamento eletrônico ABC Vet 4 (Godoy et al, 2007).…”

Section: Methodsunclassified

Avaliação de parâmetros clínicos e hematológicos de eqüinos submetidos a um programa de controle estratégico de Amblyomma cajennense (Fabricius, 1787) (Acari: Ixodidae)

Cunha¹,

Bello²,

Leite³

et al. 2008

Arq. Bras. Med. Vet. Zootec.

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Estudaram-se aspectos clínicos e hematológicos em eqüinos submetidos a um programa de controle estratégico de A. cajennense. Os tratamentos carrapaticidas foram realizados a cada sete dias e divididos em dois módulos, o primeiro com início em abril de 2004, e o segundo com início em julho do mesmo ano, utilizando-se a base química piretróide - cipermetrina na concentração de 0,015%. Além do acompanhamento clínico dos animais, foram realizados hemogramas completos antes e após o programa. As dosagens bioquímicas de bilirrubinas, gama-glutamiltransferase (GGT), aspartato aminotransferase (AST), creatina kinase (CK), proteína total, albumina e globulinas, foram realizadas antes, durante e ao final do programa. Os resultados demonstraram que houve uma melhora no quadro hematológico dos animais após o programa de controle. Os tratamentos carrapaticidas, na forma em foram aplicados, não provocaram alterações desfavoráveis nos parâmetros clínicos e hematológicos dos eqüinos. Tais informações podem ser consideradas na busca de alternativas viáveis e seguras para o controle dessa espécie de carrapato.

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Efeito do miriadenolídeo isolado de Alomia myriadenia (Asteraceae) sobre o tumor de Erlich ascítico no camundongo

Cited by 9 publications

References 20 publications

Antitumor effectiveness and toxicity of cisplatin-loaded long-circulating and pH-sensitive liposomes against Ehrlich ascitic tumor

Antitumor effectiveness and toxicity of cisplatin-loaded long-circulating and pH-sensitive liposomes against Ehrlich ascitic tumor

Parameters for evaluation of clinical trial in mice infected with Trypanosoma cruzi

Avaliação de parâmetros clínicos e hematológicos de eqüinos submetidos a um programa de controle estratégico de Amblyomma cajennense (Fabricius, 1787) (Acari: Ixodidae)

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