Efeito do Plasma Rico em Plaquetas na apoptose pós-traumática de condrócitos

Rezende, Márcia Uchôa de; Silva, Ronald Bispo Barreto da; Bassit, Ana Cristina Ferreira; Tatsui, Nelson Hidekazu; Sadigursky, David; Neto, Raul Bölliger

doi:10.1590/s1413-78522011000200008

Cited by 9 publications

(12 citation statements)

References 26 publications

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“…The use of heterologous PRP was not associated with the presence of signs suggestive of infection, similar to that reported by Rezende et al 12 who used a heterologous PRP source. Similar results were also observed with autologous PRP treatments 23,24 .…”

Section: Discussionsupporting

confidence: 87%

“…The sample size used in this study was set because of bioethical issues and to ensure that the number of animals included would provide reliable and valid scientific results in terms of statistical significance. In addition, previous work performed and published by the researchers corroborates the use of smaller sample sizes, as planned in this study 1,8,12,14,15 .…”

Section: Methodssupporting

confidence: 84%

“…In this study, where we focused on wounds not associated with compromised blood and located in regions with good blood circulation, heterologous PRP may not induce faster wound reduction or alterations in tissue contraction during the repair process, similar to the results by Bauer et al 27 In contrast, some studies that presented superior effects of PRP studied chronic wounds in peripheral regions or in patients with some type of circulatory compromise 11,12,24,28 .…”

Section: Discussionsupporting

confidence: 65%

“…Beneficial effects have been demonstrated with heterologous PRP in joint cartilage lesions 12 , and corneal ulcer healing was achieved in rabbits using a heterologous blood component associated with PRP 13 . On this basis, it is hypothesized that heterologous PRP in a gel form, when applied topically and repeatedly, can repair tissue injuries.…”

Section: Introductionmentioning

confidence: 99%

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Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits

et al. 2015

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Intellectual and scientific content of the study, surgical procedures, critical revision. ABSTRACT PURPOSE:To evaluate the potential of heterologous platelet-rich plasma (PRP) gel for surgical skin wound healing in rabbits METHODS: Blood from a single healthy dog was used for PRP production, with calcium gluconate added to the PRP to form the gel.Two surgical excisions, one to the right and the other to the left of the dorsal midline, were made in six rabbits. One side was randomly allocated to topical application of a physiological solution, and the other was allocated to treatment with heterologous PRP gel. Clinical assessments (weight, pain sensitivity, coloring, edema, hyperemia, exudation, crust, and granulation) and morphometric evaluations were performed 0, 3, 7, 10, 14, and 17 days postoperatively. Histological analysis was performed on the 17 th day. RESULTS:With the exception of the presence of a crust at day 10, clinical variables did not differ significantly between the experimental groups. In both the control and PRP-treated groups, differences were identified when comparing time-points in terms of wound area reduction. Histological results indicated no significant differences between the control group and the PRP-treated group. CONCLUSION:Heterologous platelet-rich plasma gel promoted dermal wound healing in rabbits with no adverse effects.

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Section: Discussionsupporting

confidence: 87%

Section: Methodssupporting

confidence: 84%

Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits

et al. 2015

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“…Despite this finding, the use of heterologous PRP did not result in the presence of infection or an allergic reaction, corroborating other studies that have utilized heterologous sources of PRP. 11,25 Collagen fibres were present in all the groups (Figure 2), with the highest quantities in the rabbits treated in the GA and GHO groups (P > 0.05). The same was found for tendon lesions in rabbits that were treated with PRP and porcine collagen.…”

Section: Resultsmentioning

confidence: 90%

Comparison of experimentally-induced wounds in rabbits treated with different sources of platelet-rich plasma

et al. 2015

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Over the years, autologous platelet-rich plasma (PRP) has been used to great advantage in the healing of acute and chronic wounds. However, it is impossible to use in some situations so alternative PRPs need to be considered, such as homologous or heterologous PRPs. In order to evaluate the effects of PRP in the form of autologous, heterologous and homologous gels on in vivo cutaneous wound healing, 18 animals divided into three groups of six, were used for the study, with another six animals used as PRP donors. For the heterologous group an adult, mixed breed dog was used to obtain the PRP. The wounds were induced using an 8 mm punch. The left side was treated with NaCl 0.9%® (A) and a PRP gel was applied (GA = autologous; GHE = heterologous and GHO = homologous) on the right side (B). The wounds were evaluated for a period of 17 days. On the 17th day biopsies were taken for histopathological assessment of the wounds. The percentage of contraction was evident in side B (treated), which was confirmed in the microscopic analysis of the histological sections. Thus, it was concluded that PRP, regardless of the source, improves and accelerates the healing process, demonstrating its therapeutic potential on cutaneous lesions and its use in patients with impaired wound healing.

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Platelet-rich plasma inhibits inflammatory factors and represses rheumatoid fibroblast-like synoviocytes in rheumatoid arthritis

2017

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Rheumatoid arthritis (RA) is a chronic disease affecting daily life of numerous patients, and uncontrolled proliferation of synovial fibroblasts plays vital role during the pathology of RA. Platelet-rich plasma (PRP), widely used in tissue regeneration and pain management, is rarely studied in RA. This study aims to investigate the effect of PRP on synovial fibroblasts during RA. Rheumatoid fibroblast-like synoviocyte MH7A cells were stimulated by lipopolysaccharide (LPS) to simulate RA conditions and treated with PRP, after that the concentration of inflammatory factors interleukin (IL) 1β, tumor necrosis factor alpha (TNFα) and IL6 in the supernatant of culture medium was quantified by ELISA. MTT assay, flow cytometry and tube formation assay were performed to assess changes in cell viability, apoptosis and effect on angiogenesis in vitro, respectively. Besides, the expression levels of main factors in the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) signal pathway were examined. Results showed that PRP markedly inhibited the production of IL1β, TNFα and IL6 (P < 0.05) that was stimulated by LPS. LPS promoted MH7A cell viability, inhibited apoptosis and accelerated angiogenesis in vitro, while PRP could markedly relieve these effects (P < 0.05). The mRNA and protein levels of AKT1, PI3K (p58) and nuclear factor κ beta were elevated by LPS and then suppressed by PRP (P < 0.01). This study uncovered the potential of PRP in inhibiting inflammation, repressing synovial fibroblasts and regulating the PI3K/AKT signaling, providing basic proof for future application of PRP in managing RA. Further investigation is necessary to reveal detailed mechanism of PRP.

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Efeito do Plasma Rico em Plaquetas na apoptose pós-traumática de condrócitos

Cited by 9 publications

References 26 publications

Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits

Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits

Comparison of experimentally-induced wounds in rabbits treated with different sources of platelet-rich plasma

Platelet-rich plasma inhibits inflammatory factors and represses rheumatoid fibroblast-like synoviocytes in rheumatoid arthritis

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