Efeito imunossupressor dos gangliosídeos: estudo "in vivo"

Castro, Lc; Barbiéri, Clara Lúcia; Kossel, K Von; Nigro, Amaury José Teixeira; Montero, Efs

doi:10.1590/s0102-86502000000500004

Cited by 3 publications

(2 citation statements)

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“…2 Studies demonstrated that some immunosuppressive agents act on arterial injury by decreasing or delaying intimal hyperplasia, independent of the lesion. [1][2][3] Because Gang have been shown to have an immunomodulatory effect in various kinds of immune response [4][5][6] it should be expected that they might act by delaying intimal hyperplasia. Therefore, 3 weeks after injury, there was an increase in intimal layer thickening.…”

Section: Discussionmentioning

confidence: 99%

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Gangliosides in rat femoral injury: Early effect on intimal hyperplasia

et al. 2001

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Previous studies demonstrated that some immunosuppressive agents inhibit arterial intimal hyperplasia. Our previous studies demonstrated that gangliosides (Gang) have an immunosuppressive effect on as well as an anti-inflammatory role in the wound-healing process. Therefore, we decided to examine the effect of Gang on intimal hyperplasia. Twenty Wistar isogenic rats received a transverse division of the anterior wall of the femoral artery, followed by suturing using mononylon 10-0 under surgical microscopy and were then divided into two groups: Gang group, 3 mg/kg per day of Gang, and control group, vehicle, intramuscularly from surgery to death (1 and 3 weeks, respectively). Concentric intimal hyperplasia was observed in arteries stained by hematoxylin-eosin in control and Gang groups. However, the media layer did not demonstrate any major alterations. After 3 weeks, the Gang group showed more intimal hyperplasia than the control group. Therefore, because intimal hyperplasia worsened in the presence of Gang after 3 weeks, further studies will be necessary to clarify its role in intimal proliferation.

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Section: Discussionmentioning

confidence: 99%

“…[4][5][6] Furthermore, a delay in wound healing 7 and a decrease in delayed hypersensibility response were observed. 8 Therefore, we decided to examine the effect of a Gang pool on intimal hyperplasia in the early phase of femoral artery injury in rats.…”

Section: Microsurgery 21:170-172 2001mentioning

confidence: 99%

Gangliosides in rat femoral injury: Early effect on intimal hyperplasia

et al. 2001

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Allogeneic acute rejection on fetal small‐bowel graft: role of gangliosides

Montero¹,

Steffens²,

Manna³

et al. 2006

Microsurgery

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In previous work, it was shown that gangliosides (Gang) have an inhibitory effect on lymphocyte proliferation as well as on delayed-type hypersensitivity response and mixed lymphocyte reaction. Therefore, we decided to examine the effect of gangliosides in acute allorejection after fetal intestinal transplantation. We used two female C57BL/6 mice on pregnancy day 19 as a source of fetal intestine. All animals were anesthetized with ketamine (70 mg/kg) and xylazine (10 mg/kg), intramuscularly. We harvested intestinal segments of 1 cm to transplant into BALB/c and C57BL/6 mice (male, weighing around 20 g) used as recipients. They were divided into groups of six animals each: isogeneic and allogeneic without treatment, or treated with tacrolimus 1 mg/kg/day, or gangliosides 3 and 9 mg/kg/day, during 7 days posttransplantation, intramuscularly. On postoperative day 7, intestinal grafts were collected and fixed in 10% formalin solution. Using an anesthetic overdose as euthanasia, we removed the intestinal grafts. Tissue samples were stained with hematoxylin-eosin for histological analysis regarding grafts development (D) and rejection (R) aspects. Data were analyzed by the Kruskal-Wallis test, considering P < or = 0.05 as significant. In the isogeneic and tacrolimus groups, we observed a very good degree of development (D = 9 +/- 0.5; D = 9 +/- 0.4, respectively), but a severe degree of rejection (R = 15 +/- 1.3) and a low degree of development (D = 1 +/- 0.8) in animals without treatment. The ganglioside groups showed D = 5 +/- 1.6 and R = 13 +/- 3.3, and D = 7 +/- 2.9 and R = 9 +/- 1.9, for the 3-mg and 9-mg groups, respectively. There was a statistically significant difference between the ganglioside groups and allogeneic groups without treatment. Based on the above data, we conclude that avascular fetal intestine transplantation is a good experimental model for studying immunological events, and that gangliosides only partially modulate the allorejection response, allowing intestinal development, mainly at the highest ganglioside dose. Maybe immunomodulation would be better observed by using isolated types of gangliosides or association with other immunosuppressive drugs.

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