Efeitos da Substância de Alarme no Teste Claro/Escuro no Zebrafish, Danio rerio

Mansur, Bruno de Matos; Santos, Bruno Rodrigues dos; Gouvêia, Amauri

doi:10.18561/2179-5746/biotaamazonia.v4n1p87-93

Cited by 8 publications

(5 citation statements)

References 17 publications

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“…Previous work demonstrated that dark preference is affected by different stressful manipulations, including acute exposure to AS (Mansur et al 2014;Maximino et al 2014a) and chronic unpredictable stress (Chakravarty et al 2013). The effects of AS on scototaxis were observed immediately after exposure in both cases (Mansur et al 2014;Maximino et al 2014a); in the present work, we demonstrate that AS exposure can also produce a delayed behavioral effect on this measure, potentiating the anxiogenic character of the apparatus.…”

Section: Discussionsupporting

confidence: 74%

“…shoal cohesion (Speedie and Gerlai 2008) and erratic movements in the novel tank test and group behavior task (Speedie and Gerlai 2008;Egan et al 2009;Mathuru et al 2012), increase scototaxis (preference for darkness) and freezing in the light/dark test (Mansur et al 2014;Maximino et al 2014a), to promote analgesia in zebrafish (Maximino 2011;Lima et al 2012;Maximino et al 2014a). …”

Section: Accepted Manuscriptmentioning

confidence: 99%

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Putative involvement of the nitrergic system on the consolidation, but not initiation, of behavioral sensitization after conspecific alarm substance in zebrafish

Lima

Silva

et al. 2015

Pharmacology Biochemistry and Behavior

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Stressful manipulations can sensitize the behavior of an organism, increasing anxiety-like behavior after a delay; this long-term stress sensitization can represent the pathophysiological basis of trauma- and stress-related disorders (TRSDs), of which the most prevalent is post-traumatic stress disorder (PTSD). A role for the glutamate-nitric oxide pathway in this sensitization is implied by behavioral, neurophysiological and genomic data on different species. Here, we report on the long-term sensitization of anxiety-like behavior in zebrafish and the possible participation of nitric oxide in this process. Zebrafish exposed to a conspecific alarm substance (AS) show increased anxiety-like behavior at least 24h after stimulus delivery. Blocking nitric oxide synthesis with l-NAME (5mg/kg) 30min, but not 90min, after AS exposure blocks the sensitization of scototaxis and risk assessment, while treatment 90min after exposure blocks the sensitization of thigmotaxis and erratic swimming; l-NAME was not effective when administered 30min before AS exposure. These data suggest a participation of nitric oxide in the consolidation, but not in the initiation, of behavioral sensitization after predator threat.

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Section: Discussionsupporting

confidence: 74%

Section: Accepted Manuscriptmentioning

confidence: 99%

Putative involvement of the nitrergic system on the consolidation, but not initiation, of behavioral sensitization after conspecific alarm substance in zebrafish

Lima

Silva

et al. 2015

Pharmacology Biochemistry and Behavior

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“…The raw skin extract was removed before the first day of the session, and the unused portion was pipetted and frozen at −10 °C, thawed, and immediately used in the subsequent sessions. (Ide et al, 2003; Mansur et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

Exposure effects of species bloodfin (Aphyocharax anisitsi) and jewel tetras (Hyphessobrycon eques) in different anxiety experimental models.

Silva¹,

Gouvêia²,

Brito³

2021

Psychology & Neuroscience

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Objective: This project aimed to evaluate how the fish species bloodfin (Aphyocharax anisitsi) and jewel tetras (Hyphessobrycon eques) behaved in different tests that evaluate anxiety-like behaviors. Furthermore, we assessed whether these species had ecological and/or ethological differences that affect their behavioral patterns when compared with zebrafish (Danio rerio). Methods: The evaluation of anxiety-like behaviors in the two species involved the use of 70 animals (n = 70), 40 bloodfin and 30 jewel tetras, with all fishes in the adult phase. The following tests were used to examine anxiety-like behaviors (total time in each compartment: top/bottom or dark/light, erratic swimming, freezing, and thigmotaxis): novel tank (or water column), anxiety evoked by conspecific alarm substance, and dark/light test. Statistical tests were used to assess anxiety-like behaviors. Results: Statistical differences were observed between the two species in all three tests ( p < .05). Moreover, differences were found between the data obtained through the two species and the findings in the literature for zebrafish. Conclusion: Bloodfin tetra exhibited more similarities with the behavior of zebrafish, as reported in the literature. Thus, this species is the one most suitable to replace zebrafish in anxiety-like behavior tests. Public Significance StatementIn general, the species bloodfin tetra revealed greater statistical proximity to the zebrafish's anxiety-like behaviors reported in the literature. The comparison between the two species from the Pantanal basin with zebrafish enabled the identification of ecological and ethological differences that could influence the anxiety-like behaviors of these animals.

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“…Not tested Zebrafish ↑ (Maximino et al, 2014;Lima et al, 2016;Quadros et al, 2016) 0 (Mansur et al, 2014) Fathead minnow…”

Section: Not Testedmentioning

confidence: 99%

Sensory Ecology of Ostariophysan Alarm Substances

Maximino¹,

Silva²,

Campos³

et al. 2018

Preprint

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Chemical communication of predation risk has evolved multiple times in fish species, with the conspecific alarm substance (CAS) contemporaneously being the most well understood mechanism. CAS is released after epithelial damage, usually when prey fish is captured by a predator, and elicits neurobehavioral adjustments in conspecifics which increase the probability of avoiding predation. As such, CAS is a partial predator stimulus, eliciting risk assessment-like and avoidance behaviors, and disrupting the predator sequence. The present paper reviews the distribution and putative composition of CAS in fish, and presents a model for the neural processing of these structures by the olfactory and the brain aversive systems. Applications of CAS in the behavioral neurosciences and neuropharmacology are also presented, exploiting the potential of model fish (e.g., zebrafish, guppies, minnows) on neurobehavioral research.

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Efeitos da Substância de Alarme no Teste Claro/Escuro no Zebrafish, Danio rerio

Cited by 8 publications

References 17 publications

Putative involvement of the nitrergic system on the consolidation, but not initiation, of behavioral sensitization after conspecific alarm substance in zebrafish

Putative involvement of the nitrergic system on the consolidation, but not initiation, of behavioral sensitization after conspecific alarm substance in zebrafish

Exposure effects of species bloodfin (Aphyocharax anisitsi) and jewel tetras (Hyphessobrycon eques) in different anxiety experimental models.

Sensory Ecology of Ostariophysan Alarm Substances

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