EFEK EKSTRAK ETANOL DAUN SINGKONG  (Manihot Utilissima Pohl) SEBAGAI OBAT ALTERNATIF ANTI REMATIK TERHADAP RASA SAKIT PADA MENCIT

Rikomah, Setya Enti; Elmitra, Elmitra; Yunita, Diana Gustina

doi:10.51352/jim.v3i2.119

Cited by 8 publications

(10 citation statements)

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“…A total of 1600 g of cassava leaf powder was macerated with 96% ethanol solvent to obtain 234.83 g of ethanol extract with a yield of 14.67%. This result is higher than the study by Rikomah et al [9], which produced a yield of 10.48% using 70% ethanol, contrary to this study using 96% ethanol. The simplicia and ethanol extract of cassava leaves were then subjected to phytochemical screening.…”

Section: Resultscontrasting

confidence: 95%

“…Several studies on the activity of cassava leaves have been carried out. Research by Rikomah et al [9] reported that 70% ethanol extract had an antirheumatic effect on pain in mice (Mus musculus), with the best effect at a dose of 0.65 mg. Ethanol extract has activity as an antibacterial [10,11,12], analgesic [13], antidiarrheal [14], lowers blood sugar [15], anthelmintic [16], antioxidant [10,17,18,19,20,21,22,23]. Research on cassava leaf flavonoids and antioxidant activity has not been widely reported.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant Activity of Flavonoids from Cassava Leaves (Manihot esculenta Crantz)

et al. 2022

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The community uses cassava leaves for cooking because of their high protein content, β-carotene, Fe, Mg, Zn, S, Ca, Zn, Ni, and K. Cassava leaves contain various secondary metabolites, including flavonoids, saponins, tannins, phenolics, steroids, anthocyanins, and anthraquinones. The antioxidant activity of flavonoid compounds in cassava leaves (Manihot esculenta Crantz) has been successfully investigated. This study consisted of six stages: the preparation of ethanol extract of cassava leaves, phytochemical screening, determination of total flavonoid content in the extract, isolation of flavonoids, and purity testing of flavonoid isolates using the chromatographic method, identification of the structure of pure flavonoid isolates using a UV-Vis spectrophotometer with the addition of a shear reagent, FTIR, and LC-MS/MS. As well as test the antioxidant activity using the DPPH method. The yield of ethanol extract from cassava leaf in this study was 14.67%. The results of the phytochemical screening showed that the cassava leaves and the ethanol extract of cassava leaves contained alkaloids, flavonoids, saponins, tannins, quinones, steroids, phenolics, and triterpenoids. The total content of flavonoids in the ethanol extract was 35.71 mg EQ/g extract. UV-Vis, FTIR, and LC-MS/MS analysis revealed that the flavonoid isolate was quercetin. The results of the antioxidant activity of the ethanol extract and BC isolate showed IC50 of 81.76 ± 0.505 mg/L and 77.85 ± 4.708 mg/L.

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Section: Resultscontrasting

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity of Flavonoids from Cassava Leaves (Manihot esculenta Crantz)

et al. 2022

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“…Selanjutnya, untuk mengidentifikasi perbedaan spesifik antara kelompok, pemanfaatan Uji Post Hoc Tukey HSD diperlukan. Penentuan kesimpulan ditetapkan dengan syarat bahwa nilai signifikansi p<0,05, menunjukkan adanya perbedaan antar kelompok (Rikomah et al, 2017). Berdasarkan hasil Uji Post Hoc Tukey HSD hanya terdapat perbedaan yang signifikan dengan nilai p=0,027 lebih kecil dari 0,05 pada F3 (Gel ekstrak lidah buaya 7,5%) terhadap kontrol negatif.…”

Section: Gambar 3 Grafik Persentase Penyembuhan Luka Sayatunclassified

Uji Aktivitas Penyembuhan Luka Sayat Gel Ekstrak Etanol Lidah Buaya (Aloe Vera L.) pada Mencit Putih Jantan (Mus Musculus)

Milasanti,

Widara,

Fitri

2023

jharma

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Aloe vera (Aloe vera L.) is an herbal plant that contains phytochemicals such as tannins, saponins, flavonoids, and polyphenols, which are useful in healing cut wounds. The phytochemical content has antimicrobial, antibacterial, and antiseptic properties. Several research findings show wound healing activity in experimental animals that have been treated with aloe vera plant extract. This research aimed to make ethanol extract gel from aloe vera leaves and test cuts on male white mice (Mus musculus). The type of research used is true experimental design research. An incision wound was made on the back of the mouse with a length of 1 cm and a depth of 2 mm. The test animals used were 24 animals which were divided into 6 experimental groups, namely Positive Control (Bioplacenton), Negative Control (Without extract content), Base (Without aloe vera extract and preservatives), F1 (Aloe vera extract content 2.5%), F2 (Aloe vera extract content 5%), and F3 (Aloe vera extract content 7.5%). Statistical analysis of wound healing data was carried out using the One way ANOVA (Analysis of Variants) method, while the physical examination of the gel formulation was described descriptively. The statistical test results significantly affected wound healing in male white mice (p<0.05). There is a significant difference between the negative control and F3, p-value = 0.027. The best activity in the wound healing process is F3, with an average percentage of wound healing on the 7th day, F2 on the 8th day, and F1 on the 9th day.

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“…Kandungan yang terdapat dalam daun Singkong yaitu air, fosfor, karbohidrat, kalsium, vitamin C, protein, lemak, vitamin B1, zat besi, flavonoid, saponin, tanin dan triterpenoid. 6 Penelitian terdahulu menyebutkan bahwa daun Singkong dapat berpotensi sebagai antiinflamasi. Daun Singkong memiliki efek antiinflamasi yang mirip dengan obat kimia aspirin.…”

Section: Pendahuluanunclassified

<p>Potensi ekstrak daun singkong (Manihot esculenta Crantz.) terhadap profil leukosit darah tepi model tikus disfungsi ovarium dan periodontitis</p><p>Potential of cassava leaves (Manihot esculenta Crantz.) extract on peripheral blood leukocyte profile in ovary dysfunction and periodontitis rat model</p>

et al. 2021

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Pendahuluan: Disfungsi ovarium merupakan keadaan ovarium yang mengalami kegagalan dalam sekresi hormon seks steroid. Penurunan sekresi hormon terutama estrogen dapat memicu peningkatan sitokin proinflamasi seperti IL-1, IL-6 dan TNF-α yang berperan dalam resorpsi tulang dan reaksi inflamasi periodontal. Salah satu drug of choice dari periodontitis adalah metronidazole. Penggunaan metronidazole secara sistemik dapat mengakibatkan efek samping, sehingga diperlukan bahan alternatif yang memiliki efek terapi antiinflamasi. Salah satu tanaman yang memiliki efek antiinflamasi adalah daun Singkong. Tujuan penelitian adalah menganalisis potensi ekstrak daun Singkong sebagai antiinflamasi terhadap profil leukosit darah tepi model tikus disfungsi ovarium dan periodontitis. Metode: Jenis penelitian eksperimental laboratoris dengan post-test only control group design. Sampel penelitian sebanyak 18 ekor tikus betina yang dibagi menjadi dua kelompok besar yakni kelompok disfungsi ovarium dan kelompok kecil periodontitis. Pembuatan model tikus disfungsi ovarium dan tikus periodontitis dilakukan selama 28 hari. Setelah masing-masing kelompok dibagi menjadi 3 kelompok yang diberikan perlakuan aquades, metronidazole dan ekstrak daun Singkong selama 7 hari. Setiap tikus diambil darahnya melalui plexus infraorbitalis pada hari ke-1, hari ke-3 dan hari ke-7. Darah yang diambil selanjutnya dilakukan perhitungan jumlah sel leukosit darah tepi menggunakan kamar hitung dan perhitungan jenis dengan cara visual. Hasil: Hasil uji LSD menunjukkan bahwa terdapat perbedaan bermakna jumlah leukosit darah tepi antar hari pada kelompok tikus yang mengalami disfungsi ovarium (p≤0,05). Simpulan: Ekstrak daun Singkong (Manihot esculenta Crantz) dapat menurunkan profil leukosit perifer model tikus disfungsi ovarium dan periodontitis.Kata kunci: Disfungsi ovarium, periodontitis, profil leukosit perifer, ekstrak daun singkong. ABSTRACTIntroduction: Ovarian dysfunction is when the ovaries fail in the secretion of steroid sex hormones. Decreased secretion of hormones, especially estrogen, can trigger an increase in pro-inflammatory cytokines such as IL-1, IL-6 and TNF-α, which play a role in bone resorption and periodontal inflammatory reactions. One drug of choice for periodontitis is metronidazole. Systemic use of metronidazole can cause side effects, so an alternative material with a therapeutic anti-inflammatory effect is needed. One of the plants that have an anti-inflammatory effect is cassava leaves. The research objective was to analyse the potential of cassava leaf extract as an anti-inflammatory against the peripheral blood leukocyte profile in a mouse model of ovarian dysfunction and periodontitis. Methods: This was an experimental laboratory study with a post-test only control group design. The research sample consisted of 18 female rats divided into two large groups: the ovarian dysfunction group and the small periodontitis group. The making of mouse models of ovarian dysfunction and periodontitis rats was carried out for 28 days. After each group was divided into three groups treated with distilled water, metronidazole and cassava leaf extract for seven days. Blood was drawn from each mouse through the infraorbital plexus on day 1, day three, and 7. The blood that was taken was then performed to calculate the number of peripheral blood leukocytes using the counting room and the calculation of the type by visual means. Results: The results of the LSD test showed that there was a significant difference in the number of peripheral blood leukocytes between days in the group of rats with ovarian dysfunction (p≤0.05). Conclusion: Cassava leaf extract (Manihot esculenta Crantz) can reduce the profile of peripheral leukocytes in a mouse model of ovarian dysfunction and periodontitis.Keywords: Ovarian dysfunction, periodontitis, peripheral leukocytes profile, cassava leaf extract.

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EFEK EKSTRAK ETANOL DAUN SINGKONG (Manihot Utilissima Pohl) SEBAGAI OBAT ALTERNATIF ANTI REMATIK TERHADAP RASA SAKIT PADA MENCIT

Cited by 8 publications

References 0 publications

Antioxidant Activity of Flavonoids from Cassava Leaves (Manihot esculenta Crantz)

Antioxidant Activity of Flavonoids from Cassava Leaves (Manihot esculenta Crantz)

Uji Aktivitas Penyembuhan Luka Sayat Gel Ekstrak Etanol Lidah Buaya (Aloe Vera L.) pada Mencit Putih Jantan (Mus Musculus)

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