Efek Hipoglikemik Kecambah Beras Merah pada Tikus yang Diinduksi STZ-NA dengan Parameter Kadar Insulin, Indeks HOMA-IR dan HOMA β (Hypoglycemic Effect of Red Rice Germ on Insulin Levels, HOMA-IR, and HOMA β Index of STZ-NA Induced Rats)

Abstract

ABSTRAKProses perkecambahan biji-bijian seperti beras dapat meningkatkan beberapa nilai gizi seperti asam amino dan serat pangan. Beras merah dan kecambahnya diyakini mampu menurunkan glukosa darah pada penderita diabetes melitus (DM). Tujuan penelitian ini adalah mengevaluasi efek hipoglikemik kecambah beras merah pada tikus diabetes yang diinduksi STZ-NA terhadap kadar glukosa darah, insulin, serta indeks HOMA-IR dan HOMA β. Penelitian ini bersifat eksperimental in vivo pada hewan coba tikus Wistar jantan usia 10 minggu sebanyak 24 ekor dengan desain penelitian randomized post test only control group. Tikus dibagi menjadi 4 kelompok, masing-masing 1 kelompok tanpa induksi STZ-NA dengan diet standar dan 3 kelompok diinduksi STZ-NA dengan diet standar, beras merah dan kecambah beras merah. Percobaan dilakukan selama 6 minggu. Hasil penelitian menunjukkan kecambah beras merah mampu menurunkan kadar glukosa darah sebesar 61,88 % dan nilai HOMA-IR (parameter resistensi insulin) 56,82 %. Kadar insulin meningkat 16,35 % dan HOMA β 763,6 %. Disimpulkan, kecambah beras merah mampu menurunkan kadar glukosa darah dan kondisi resistensi insulin tikus DM, dan meningkatkan sel beta pankreas.Kata kunci: HOMA-IR; HOMA β; hipoglikemik; insulin; kecambah beras merah ABSTRACTThe process of germination of grains such as rice could increase some nutritional values of amino acids and dietary fiber. Red rice and its sprouts are believed to be able to decrease blood glucose in patients with diabetes mellitus (DM). The aim of this study was to evaluate the hypoglycemic effect of red rice sprouts in STZ-NA induced diabetic rats on blood glucose level, insulin level, and HOMA-IR and HOMA-β index. This experimental study was conducted based on randomized post test only control group design using 24 male Wistar rats aged 10 weeks. Rats were divided into 4 groups, one group without induction of STZ-NA fed with a standard diet (control) and three groups of STZ-NA induced with a standard diet, red rice and red rice germ. Experiments were conducted for 6 weeks. The results showed that sprouted red rice lowered blood glucose levels by 61.88 % and the value of HOMA-IR (insulin resistance parameters) by 56.82%. Insulin level increased by 16.35 % and HOMA-β by 763.6 %. This study showed that red rice germ was able to ddecrease blood glucose levels and insulin resistance of DM rats and increase strength of the pancreatic beta cells.

Antidiabetic Effects of Red Rice Bran in The Rat Models of Diabetes

“…This is related to disturbances in cells that reduce insulin synthesis and secretion due to STZ-NA induction in this study was evidenced by the P1 group that was induced by STZ-NA and fed standard feed without any intervention having decreased insulin levels. This statement is in line with previous studies that proved insulin levels in the DM rat group after STZ-NA induced decreased compared to the group without induction (Nurhidajah & Nurrahman, 2017).…”

“…In another study, giving germinated black rice to STZ-NA induced rats could increase insulin levels by 16.35% (Nurhidajah & Nurrahman, 2017). In this study, the group of DM rats after the intervention showed an increase in insulin levels compared to the group of DM rats without intervention.…”

Anti-diabetic Activity of the Red Dragon Fruit Peel (Hylocereus polyrhizus) in Ethanol Extract against Diabetic Rats

“…Furthermore, this study reported that on day 3 (72 hours after STZ-NA induction), the blood glucose levels in the treated rats were above 200 mg/dl, ranging from 379.80-416.07 mg/dl, which indicated that all rats were diabetes mellitus. In accordance with Anwer (2014) 38 ; Nurhidajah & Nurrahman(2016), 40 it was demonstrated that blood glucose levels would be >200 mg/dl after 72 hours STZ-NA administration, and it can be claimed that rats were in diabetes mellitus type 2 already.…”

Antihyperglycemic effect of patikan kebo in the rat models of diabetic

“…Insulin resistance was determined using the Homeostasis Model Assessment Insulin Resistance (HOMA-IR) formula (Byun AR et al, 2015), and to determine the level of pancreatic β-cell function strength using the Homeostasis Model Assessment of Pancreatic Beta Cell Function (HOMAβ) formula (Nurhidajah and Nurrahman, 2016).…”