Nowadays, organic waste from plants cannot be processed optimally. One of the solutions to protect the environment is to thoroughly process organic waste into an alternative eco-enzyme that is useful in making biodegradable detergents. Alternative sources of biosurfactant production can be substituted with alternative materials that are more environmentally friendly, such as Moringa leaves (Moringa oleifera). The purpose of this study was to: (1) produce biodegradable detergents processed through the synthesis of eco-enzymes and Moringa leaves surfactants, (2) find out the value of pH test, organoleptic test, and antibacterial test, and (3) explore the Moringa leaves’ antibacterial in biodegradable detergent production. The method used in this study included maceration, filtration, and evaporation using a rotary evaporator to get pure Moringa surfactants. Detergent is made by adding eco-enzymes which have been synthesized through a fermentation from organic matter. The quality control of products including organoleptic tests, pH, and antibacterial tests which are then compared with commercial detergents. Optimum biosurfactant results were obtained at an extract concentration of 45%. Liquid detergent showed physicochemical properties produced in accordance with SNI standards with a pH of 11. The results of the biosurfactant antibacterial test were 15.9 mm, 14.6 mm, and 15.2 mm. This study shows that the quality of the detergent is good, which is categorized as strong antibacterial strength.