Eff ects of hemin, a heme oxygenase-1 inducer in L-arginine-induced acute pancreatitis and associated lung injury in adult male albino rats

Aziz, Neven Makram; Kamel, Maha Yehia; Rifaai, Rehab Ahmed

doi:10.1515/enr-2017-0003

Cited by 25 publications

(23 citation statements)

References 41 publications

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“…The results of the present study demonstrated that administration of L-arginine to rats produced severe AP, which was confirmed with histopathological examination. In addition, marked increase in the serum levels of pancreatic enzymes, NO, CORT and TNF-α, and pancreatic MDA and iNOS levels were detected what is in a line with previous studies (Ucmak et al 2016;Yilmaz et al 2016;Aziz et al 2017).…”

Section: Discussionsupporting

confidence: 91%

“…An elevated level of NO could be ascribed to induction of iNOS that was evoked by inflammatory cytokines. NO reacts with superoxide anion to form peroxynitrite that can react directly with lipid, protein and DNA resulting in cellular dysfunction and tissue injury what has been demonstrated in many studies (Biradar and Veeresh 2013;Al-Malki 2015;Hasan et al 2015;Aziz et al 2017).…”

Section: Discussionmentioning

confidence: 91%

“…Furthermore, in the NO-independent pathway, L-arginine is hydrolyzed by arginase to urea and L-ornithine which induce severe AP (Foster 2014;Kui et al 2014). Moreover, L-arginine may also stimulate the induction of AP through increased production of cytokines, such as TNF-α and IL-6 (Aziz et al 2017).…”

Section: Discussionmentioning

confidence: 99%

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Comparing the preventive effect of sodium hydrosulfide, leptin, and curcumin against L-arginine induced acute pancreatitis in rats: role of corticosterone and inducible nitric oxide synthase

Ragy

Ali

Toni

2019

Endocrine Regulations

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Objectives. Acute pancreatitis (AP) is a life-threatening condition. Using antioxidants in AP is insufficient and conflicting. Therefore, this study compared the effect of hydrogen sulfide (H2S) donor, sodium hydrosulfide (NaHS), leptin or curcumin pretreatment on AP induced by L-arginine.Methods. Forty adult male rats were used and classified into: 1) control; 2) AP group [each rat was intraperitoneally (i.p.) injected with 2 doses of L-arginine of 250 mg/100 g body weight (b.w.) with an interval of 1 h]; 3) NaHS+AP group (each rat was i.p. injected with 10 mg/kg b.w. of NaHS 1 h before induction of AP); 4) leptin+AP group (each rat was pretreated with 10 μg/kg b.w. of leptin 30 min before induction of AP; and 5) curcumin+AP group (in which rats were i.p. injected with 150 mg/kg b.w. of curcumin 30 min before induction of AP). Serum amylase, lipase, nitric oxide (NO), tumor necrosis factor alpha (TNF-α), and corticosterone (CORT) levels were assayed. In addition, pancreatic tissues were obtained for histopathological examination and malondialde-hyde (MDA), total antioxidant capacity (TAC), and inducible nitric oxide synthase (iNOS) levels were measured.Results. All AP treated groups showed significant decrease in serum levels of pancreatic enzymes, NO, and TNF-α, and pancreatic MDA and iNOS levels, while TAC levels were significantly increased. NaHS caused more limitation of inflammation than leptin and curcumin by affecting iNOS. Leptin was more potent than curcumin due to the stimulatory effect of leptin on glucocorticoid release to counteract inflammation.Conclusions. NaHS was more effective in AP amelioration than the leptin and curcumin.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 91%

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Comparing the preventive effect of sodium hydrosulfide, leptin, and curcumin against L-arginine induced acute pancreatitis in rats: role of corticosterone and inducible nitric oxide synthase

Ragy

Ali

Toni

2019

Endocrine Regulations

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“…HMGB1 could activate toll-like receptors 4 (TLR4) signaling pathway, which is indispensable for the inflammatory process, and the specific inhibitors of HMGB1 or TLR4 (such as eritoran and [(S,R)-3-phenyl-4,5-dihydro-5-isoxasole acetic acid)] could alleviate the inflammation and improve the prognosis (33)(34)(35). The present study revealed that TNF-α and HMGB1 were upregulated in the SAP and SAP + BDG groups compared with the control groups, but BDG decreased the levels of TNF-α significantly and HMGB1 at 6 h. HO-1 is considered as an important anti-inflammatory factor, which is responsible for cytothesis and tissue protection (36). HO-1 could exert anti-inflammatory effects via the production of carbon monoxide (CO), which may be a possible factor of early treatment of SAP, while CO has been proven effective in some immuno-inflammatory and autoimmune diseases (37,38).…”

Section: Discussionmentioning

confidence: 54%

Sustained bile drainage decreases the organs injuries via inflammation‑associated factors modulation in a severe acute pancreatitis rat model

et al. 2019

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The timely and effective treatment for severe acute pancreatitis (SAP) is favorable to prognosis. Decompression of the bile duct might be a feasible way to decrease the progression of SAP. The present study investigated the effects of sustained bile external drainage on organs injury caused by SAP in Sprague-Dawley (SD) rats and the mechanisms involved. A total of 72 female SD rats weighting 190-230 g were randomly divided into four groups (n=18): Sham operation group (SOG), SOG + bile drainage group (BDG), SAP group, and SAP + BDG. Sodium taurocholate solution (4%; 1 mg/kg body weight) was used to set up SAP model via injection of retrograde puncture of biliopancreatic duct through the duodenum. A cannula was inserted into the bile duct and fixed externally to establish BDG model. At each time points (t=3, 6, 12; n=6), tissues from the liver, lung, and pancreas, and blood samples were collected. Serum amylase (AMY) was analyzed in all the samples. The levels of tumor necrosis factor-α (TNF-α), heme oxygenase-1 (HO-1), interleukin-10 (IL-10) and high mobility group box 1 (HMGB1) were detected by ELISA. Hematoxylin-eosin staining was performed to observe the histopathological changes, and nuclear transcription factor (NF)-κB-p65 levels in the pancreas were analyzed by western blotting. The data indicated that BDG alleviated the SAP progression and multiple organs injuries. Meanwhile, the histopathological changes of the pancreas, liver, and lungs were improved by BDG. BDG decreased the pathological scores of pancreas significantly (P<0.05). The levels of AMY, TNF-α, HMGB1, and NF-κB-p65 were significantly downregulated by BDG (P<0.05), while the level of HO-1 was upregulated and IL-10 was unchanged. In summary, BDG may attenuate the multiple organs injuries caused by SAP via downregulation of TNF-α, HMGB1, NF-κB-p65 and upregulation of HO-1.

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“…This is in accordance with El-Rahman et al (2011) [21] and Hasan et al (2015) [22] . L-arginine produces biochemical and histological changes like that of human AP [23] . It distorts the membranes of zymogen granules and release their enzymes to the interstitium causing inflammation [22] .…”

Section: Discussionmentioning

confidence: 92%

Comparative Histological Study on The Effect of Bone Marrow-Derived Mesenchymal Stem Cells Versus Wheat Germ Oil on Acute Pancreatitis in A Rat Model

Aboulkhair

Nasr

Radwan

et al. 2019

Egyptian Journal of Histology

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Background: Acute pancreatitis (AP) is a common inflammatory disorder of digestive system. Mesenchymal stem cells (MSCs) and Wheat germ oil (WGO) could improve AP through their anti-inflammatory and antioxidant effects. Objective: Evaluate and compare the possible therapeutic effects of Bone marrow derived Mesenchymal Stem Cells (BMSCs) versus WGO on AP. Materials and Methods: 47 adult male albino rats were divided into 4 groups. Control group I (no.=12). AP was induced in the remaining 35 rats by a single intra peritoneal (I.P) injection of L-arginine (250 mg/100g). 5 rats died within the 1st hour after AP induction, the rest were divided randomly into group II (AP group; no.=10) that received no treatment, group III (BMSCs group; no.=10) and group IV (WGO group; no.=10). One hour after AP induction, group III was injected I.P. by 1ml of PKH26 labeled BMSCs (1x106 cells/ml) and group IV received WGO in a dose of 3 ml/kg body weight by oral gavage every 24 h for 3 successive days. Blood samples were collected 24 hours and on the 4 th day after AP induction for biochemical assessment of serum amylase, lipase, interleukin-1β and interleukin-10. Then, animals were sacrificed and specimens from the pancreas were prepared for Hematoxylin and eosin (HandE) stain and immune-histochemical staining using inducible Nitric Oxide Synthase (iNOS) and insulin antibodies. Morphometric measurements using image analyzer were done. Results: Group II showed extensive pancreatic damage associated with increase in serum amylase, lipase and interleukin-1B levels and reduction in interleukin-10 level. A significant increase in the area % of iNOS immunostaining and non-significant change in insulin immunostaining were detected. On the other hand, BMSCs group and WGO group showed improvement in the biochemical, histological and immunohistochemical results with better results in BMSCs group. Conclusion: BMSCs possess better therapeutic efficacy in treating AP compared with WGO.

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Eff ects of hemin, a heme oxygenase-1 inducer in L-arginine-induced acute pancreatitis and associated lung injury in adult male albino rats

Cited by 25 publications

References 41 publications

Comparing the preventive effect of sodium hydrosulfide, leptin, and curcumin against L-arginine induced acute pancreatitis in rats: role of corticosterone and inducible nitric oxide synthase

Comparing the preventive effect of sodium hydrosulfide, leptin, and curcumin against L-arginine induced acute pancreatitis in rats: role of corticosterone and inducible nitric oxide synthase

Sustained bile drainage decreases the organs injuries via inflammation‑associated factors modulation in a severe acute pancreatitis rat model

Comparative Histological Study on The Effect of Bone Marrow-Derived Mesenchymal Stem Cells Versus Wheat Germ Oil on Acute Pancreatitis in A Rat Model

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