Effect and Analysis of Bacterial Lysates for the Treatment of Recurrent Urinary Tract Infections in Adults

Ahumada-Cota, Ricardo E; Hernández, Ulises Reyes; Milián-Suazo, Feliciano; Chávez-Berrocal, María E; Navarro‐Ocaña, Arturo; Martínez-Gómez, Daniel; Patiño-López, Genaro; Salazar-Jiménez, E P; Eslava, Carlos

doi:10.3390/pathogens9020102

Cited by 16 publications

(36 citation statements)

References 52 publications

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“…Urine sampling was performed monthly according to traditional proceedings and delivered in less than 4 h to the Bacterial Pathogenicity Laboratory for analysis [ 33 , 34 , 35 ]. All urine samples were subjected to a urinalysis with a reactive test strip for 10 parameters (Mission, San Diego, CA, USA) and a bacterial culture.…”

Section: Methodsmentioning

confidence: 99%

Prospective Study in Children with Complicated Urinary Tract Infection Treated with Autologous Bacterial Lysates

et al. 2021

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Antimicrobial bacteria resistance is an important problem in children with recurrent urinary tract infections (rUTI), thus it is crucial to search for alternative therapies. Autologous bacterial lysates (ABL) may be a potential treatment for rUTI. Twenty-seven children with rUTI were evaluated for one year, urine and stool cultures were performed, 10 colonies of each culture were selected and those identified as Escherichia coli were characterized by serology. For patients who presented ≥105 UFC/mL, an ABL was manufactured and administered orally (1 mL/day) for a month. Twelve children were monitored for ≥1-year, 218 urine and 11 stool samples were analyzed. E. coli (80.5%) was the main bacteria isolated from urine and feces (72%). E. coli of classical urinary serotypes (UPEC), O25:H4, O75:HNM, and O9:HNM were identified in patients with persistent urinary infection (pUTI). In 54% of patients treated with ABL, the absence of bacteria was observed in urine samples after 3 months of treatment, 42% of these remained without UTI between 10–12 months. It was observed that the use of ABL controlled the infection for almost 1 year in more than 60% of the children. We consider it necessary to develop a polyvalent immunogen for the treatment and control of rUTI.

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Section: Methodsmentioning

confidence: 99%

Prospective Study in Children with Complicated Urinary Tract Infection Treated with Autologous Bacterial Lysates

et al. 2021

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“…One hundred fifty MDR E . coli isolates, previously serotyped and characterized by MDR patterns, were used in this study (Ahumada-Cota et al 2020 ). The strains were initially grouped according to their origin: 50 strains isolated from stool cultures, 50 strains from recurrent UTIs at the “Federico Gómez” Children’s Hospital (Mexico City), and 50 strains from acute UTIs at the Family Medicine Unit No.…”

Section: Methodsmentioning

confidence: 99%

|Isolation and characterization of novel bacteriophages as a potential therapeutic option for Escherichia coli urinary tract infections

González-Villalobos

Ribas‐Aparicio

Montealegre

et al. 2021

Appl Microbiol Biotechnol

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Urinary tract infections (UTIs) are mainly caused by uropathogenic Escherichia coli (UPEC), whose impact can be exacerbated by multidrug-resistant (MDR) strains. Effective control strategies are, therefore, urgently needed. Among them, phage therapy represents a suitable alternative. Here, we describe the isolation and characterization of novel phages from wastewater samples, as well as their lytic activity against biofilm and adherence of UPEC to HEp-2 cells. The results demonstrated that phage vB_EcoM-phiEc1 (ϕEc1) belongs to Myoviridae family, whereas vB_EcoS-phiEc3 (ϕEc3) and vB_EcoS-phiEc4 (ϕEc4) belong to Siphoviridae family. Phages showed lytic activity against UPEC and gut commensal strains. Phage ϕEc1 lysed UPEC serogroups, whereas phages ϕEc3 and ϕEc4 lysed only UTI strains with higher prevalence toward the O25 serogroup. Moreover, phages ϕEc1 and ϕEc3 decreased both biofilm formation and adherence, whereas ϕEc4 was able to decrease adherence but not biofilm formation. In conclusion, these novel phages showed the ability to decrease biofilm and bacterial adherence, making them promising candidates for effective adjuvant treatment against UTIs caused by MDR UPEC strains. Key points Phage with lytic activity against MDR UPEC strains were isolated and characterized under in vitro conditions. A novel method was proposed to evaluate phage activity against bacterial adherence in HEp-2 cell.. Phages represent a suitable strategy to control infections caused by MDR bacteria.

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“…In this way, Montorsi et al [32] have proved, in a pilot study, the efficacy of the combined intake of Lactobacillus rhamnosus SGL06, cranberry and vitamin C for the prevention of recurrent UTIs. Polewski et al [90] have also evaluated the impact of lactobacilli in combination with cranberry A-type proanthocyanidins on reducing the invasiveness of extra-intestinal pathogenic E. coli (ExPEC) to intestinal cells. The exploration and incorporation of advanced probiotic formulations and microbiome-targeted treatment strategies within urology is warranted [38].…”

Section: Combined Action Of Cranberry and Probiotics Against Utismentioning

confidence: 99%

Cranberry Polyphenols and Prevention against Urinary Tract Infections: Relevant Considerations

2020

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Cranberry (Vaccinium macrocarpon) is a distinctive source of polyphenols as flavonoids and phenolic acids that has been described to display beneficial effects against urinary tract infections (UTIs), the second most common type of infections worldwide. UTIs can lead to significant morbidity, especially in healthy females due to high rates of recurrence and antibiotic resistance. Strategies and therapeutic alternatives to antibiotics for prophylaxis and treatment against UTIs are continuously being sought after. Different to cranberry, which have been widely recommended in traditional medicine for UTIs prophylaxis, probiotics have emerged as a new alternative to the use of antibiotics against these infections and are the subject of new research in this area. Besides uropathogenic Escherichia coli (UPEC), the most common bacteria causing uncomplicated UTIs, other etiological agents, such as Klebsiellapneumoniae or Gram-positive bacteria of Enterococcus and Staphylococcus genera, seem to be more widespread than previously appreciated. Considerable current effort is also devoted to the still-unraveled mechanisms that are behind the UTI-protective effects of cranberry, probiotics and their new combined formulations. All these current topics in the understanding of the protective effects of cranberry against UTIs are reviewed in this paper. Further progresses expected in the coming years in these fields are also discussed.

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Effect and Analysis of Bacterial Lysates for the Treatment of Recurrent Urinary Tract Infections in Adults

Cited by 16 publications

References 52 publications

Prospective Study in Children with Complicated Urinary Tract Infection Treated with Autologous Bacterial Lysates

Prospective Study in Children with Complicated Urinary Tract Infection Treated with Autologous Bacterial Lysates

|Isolation and characterization of novel bacteriophages as a potential therapeutic option for Escherichia coli urinary tract infections

Cranberry Polyphenols and Prevention against Urinary Tract Infections: Relevant Considerations

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